Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in kumquat

Abstract

Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants. In this study, we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat [Fortunella margarita (Lour.) Swingle] contributes to its freezing tolerance by minimizing protein degradation. Firstly, we found that only cold-acclimated kumquat plants, despite extensive leaf cellular damage during freezing, were able to resume their normal growth upon stress relief. To dissect the impact of cold acclimation on this anti-freezing performance, we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation (4 °C), freezing treatment (−10 °C) and post-freezing recovery (25 °C). FmASP (Against Serine Protease) and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature treatment. FmASP was further characterized as a robust inhibitor of multiple proteases. In addition, heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing tolerance. Finally, we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation, thereby maintaining essential cellular function for post-freezing recovery. These findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.

A new disease of kumquat (Fortunella margarita) caused by Colletotrichum karsti: twig and branch dieback

“…Kumquat has gained significant economic importance due to its ability to tolerate extreme climatic conditions (e.g. freezing temperatures), compared to other citrus species (Morton, 1987;Yang et al, 2023), and for its agronomic traits and nutritional properties. Severe symptoms of twig and branch dieback on kumquat trees were reported for the first time in Sicily from March to July of 2022 after low temperatures, windstorms, and rainfall events.…”