Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model

Tronci, Giuseppe; Yin, Jie; Holmes, Róisín; He, Liang; Russell, Stephen J.; Wood, David

doi:10.1039/c6tb02268e

Cited by 35 publications

(36 citation statements)

References 61 publications

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“…Atelocollagen “as received” was the thickest collagen sample and revealed the highest swelling degree and temperature dependency of all collagen samples, with a swelling degree at RT almost twice as high as at 37 °C. Large swelling ratios of Atelocollagen have been reported previously [ 43 , 44 ]. As revealed by light microscopy and SEM, Atelocollagen possessed much larger cavities than Collagen Solutions and Viscofan samples ( Figure 3 B).…”

Section: Discussionmentioning

confidence: 60%

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Eckes

Braun

Wack

et al. 2020

IJMS

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For medical application, easily accessible biomaterials with tailored properties are desirable. Collagen type I represents a biomaterial of choice for regenerative medicine and tissue engineering. Here, we present a simple method to modify the properties of collagen and to generate collagen laminates. We selected three commercially available collagen sheets with different thicknesses and densities and examined the effect of rose bengal and green light collagen crosslinking (RGX) on properties such as microstructure, swelling degree, mechanical stability, cell compatibility and drug release. The highest impact of RGX was measured for Atelocollagen, for which the swelling degree was reduced from 630% (w/w) to 520% (w/w) and thickness measured under force application increased from 0.014 mm to 0.455 mm, indicating a significant increase in mechanical stability. Microstructural analysis revealed that the sponge-like structure was replaced by a fibrous structure. While the initial burst effect during vancomycin release was not influenced by crosslinking, RGX increased cell proliferation on sheets of Atelocollagen and on Collagen Solutions. We furthermore demonstrate that RGX can be used to covalently attach different sheets to create materials with combined properties, making the modification and combination of readily available sheets with RGX an attractive approach for clinical application.

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Section: Discussionmentioning

confidence: 60%

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Eckes

Braun

Wack

et al. 2020

IJMS

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“…The modulation of collagen secretion by fibroblasts in hydrogels for understanding mechanical cues that can alter wound healing using non‐linear optics is a relatively new approach for tissue engineering. Collagen gels have been studied in the past for their efficacy in hastening the wound healing process toward granular tissue formation . The structure and orientation of collagen fibers have been studied for their influence on cell‐mediated migration, which could play an important role in the remodeling of the wound site .…”

Section: Discussionmentioning

confidence: 99%

Collagen organization deposited by fibroblasts encapsulated in pH responsive methacrylated alginate hydrogels

Boddupalli

Bratlie

2018

J Biomedical Materials Res

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The pH of dermal wounds shifts from neutral during the inflammatory phase to slightly basic in the tissue remodeling phase. Stage specific wound treatment can be developed using environmentally responsive alginate hydrogels. The chemistry of these networks dictates swelling behavior. Here, we fabricated alginate hydrogels using chain growth, step growth, and combined mixed mode gelation methods to crosslink methacrylated alginate (ALGMA) and gain control over swelling responses. Methacrylation of the alginate network was confirmed through NMR spectroscopy. Strontium cations were introduced to fabricate stiffer, dually crosslinked hydrogels. Dual crosslinking significantly decreased the swelling response over the pH range of 3-9 for step growth and chain growth hydrogels, with no impact on mixed mode hydrogels. The extent of crosslinking altered the hydrogel degradation profiles under accelerated degradation conditions. Encapsulated NIH/3T3 fibroblasts in the different ALGMA hydrogels remained viable with greater cell proliferation in the stiffer gels. Collagen organization deposited by the NIH/3T3 fibroblasts was monitored using second harmonic generation (SHG) microscopy and was influenced by the crosslinking mechanism. Ionic chain growth and ionic mixed mode crosslinked ALGMA hydrogels caused relatively isotropic collagen organization, particularly 10 days post-cell encapsulation. Principal component analysis (PCA) was employed to uncover correlations between the observed properties. The ability of these environmentally responsive gels to induce isotropic collagen and respond to pH changes means they hold promise as phase specific wound dressings. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2934-2943, 2018.

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“…It indicated that dopamine barely releases from the PLGA fibers. The release of drug is often accompanied by material degradation [24]. However, the situation was different in the polydopamine-assisted system.…”

Section: Kinetics Of Absorption and Releasementioning

confidence: 99%

Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion

et al. 2020

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Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro. To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate (p<0.01), further improved the hydrophilicity of substrates (p<0.05). Furthermore, the wound closure process was much more accelerated in the pDA/ PLGA/PDGF-BB group (p<0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α (p<0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF (p<0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.

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Protease-sensitive atelocollagen hydrogels promote healing in a diabetic wound model

Abstract: Protease-sensitive atelocollagen hydrogels were chemically designed to promote accelerated wound healingin vivocompared to a dressing gold standard.

Cited by 35 publications

References 61 publications

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Collagen organization deposited by fibroblasts encapsulated in pH responsive methacrylated alginate hydrogels

Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion

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