Proteases secreted by <i>Gnathostoma binucleatum</i> degrade fibronectin and antibodies from mammals

Vibanco-Pérez, Norberto; Durán‐Avelar, Ma. de Jesús; Zambrano‐Zaragoza, José Francisco; Ventura-Ramón, Guadalupe Herminia

doi:10.1515/helmin-2015-0005

Cited by 2 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A metalloendopeptidase and a serine carboxypeptidase were discovered as predominant proteases in the G. spinigerum secreted product. Metalloproteases released by G. binucleatum aL3 were found to degrade gelatin, fibronectin, and antibodies in a prior study, suggesting that they may play a role in Gnathostoma tissue invasion, migration, and immune evasion [ 47 ]. For that reason, metalloprotease inhibitors may reduce collateral tissue damage of gnathostomiasis.…”

Section: Proteomics For Identifying Immunodiagnostic Candidates and Drug Targets Of G Spinigerummentioning

confidence: 99%

Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development

et al. 2021

View full text Add to dashboard Cite

Gnathostoma spinigerum is the most common cause of gnathostomiasis in humans. It has a complex life cycle, which requires two intermediate hosts and a definitive host, and poses a high risk for zoonosis. Definitive prognosis of gnathostomiasis relies mainly on the isolation of advanced-stage larvae (aL3), which is very challenging especially if the aL3 is sequestered in difficult-to-reach organs. There is also a lack of a confirmatory diagnostic test for gnathostomiasis. With the ongoing advancement of proteomics, a potential diagnostic approach is underway using immunoproteomics and immunodiagnostics. In addition to this, the employment of mass spectrometry could further elucidate not only understanding the biology of the parasite but also determining potential targets of prospective drugs and vaccines. This article reports the past, present, and future application of proteomics in the study of gnathostomiasis.

show abstract

Section: Proteomics For Identifying Immunodiagnostic Candidates and Drug Targets Of G Spinigerummentioning

confidence: 99%

Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development

et al. 2021

View full text Add to dashboard Cite

show abstract

Transcriptome and excretory–secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development

et al. 2019

View full text Add to dashboard Cite

Background: Gnathostoma spinigerum is a harmful parasitic nematode that causes severe morbidity and mortality in humans and animals. Effective drugs and vaccines and reliable diagnostic methods are needed to prevent and control the associated diseases; however, the lack of genome, transcriptome, and proteome databases remains a major limitation. In this study, transcriptomic and secretomic analyses of advanced third-stage larvae of G. spinigerum (aL3Gs) were performed using next-generation sequencing, bioinformatics, and proteomics. Results: An analysis that incorporated transcriptome and bioinformatics data to predict excretory–secretory proteins (ESPs) classified 171 and 292 proteins into classical and non-classical secretory groups, respectively. Proteins with proteolytic (metalloprotease), cell signaling regulatory (i.e., kinases and phosphatase), and metabolic regulatory function (i.e., glucose and lipid metabolism) were significantly upregulated in the transcriptome and secretome. A two-dimensional (2D) immunomic analysis of aL3Gs-ESPs with G. spinigerum-infected human sera and related helminthiases suggested that the serine protease inhibitor (serpin) was a promising antigenic target for the further development of gnathostomiasis immunodiagnostic methods. Conclusions: The transcriptome and excretory–secretory proteome of aL3Gs can facilitate an understanding of the basic molecular biology of the parasite and identifying multiple associated factors, possibly promoting the discovery of novel drugs and vaccines. The 2D-immunomic analysis identified serpin, a protein secreted from aL3Gs, as an interesting candidate for immunodiagnosis that warrants immediate evaluation and validation.

show abstract

Proteases secreted by Gnathostoma binucleatum degrade fibronectin and antibodies from mammals

Cited by 2 publications

References 30 publications

Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development

Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development

Transcriptome and excretory–secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development

Contact Info

Product

Resources

About