Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves

Abstract

The ultraviolet-B (UV-B) radiation can affect gene expression of plant in growth and development. Winter wheat (Triticum aestivum), as a kind of economic crop cultured in the Northern China, is also damaged by present-day ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could alleviate cell damage caused by UV-B radiation in plants. To get the proteomic changes of wheat alleviated by He-Ne lasers, we studied protein expression profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel electrophoresis), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, in which they were confirmed to be winter wheat proteins. The results showed: 1) the proteomic changes between the ultraviolet-B radiation group and normal light group were significantly different on sixth day; 2) The expression of some proteins such as catalase, DNA polymerase and reduced glutathione declines at the ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group. This indicates UV-B could regulate genes encoding proteins in Winter wheat leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could significantly alleviate UV-B-induced damage in wheat.

Laser Irradiation Effects at Different Wavelengths on Phenology and Yield Components of Pretreated Maize Seed

“…Laser technology can be a convenient option to be incorporated into systems of agricultural production. Therefore, more attention was given in recent years to physical factors that may be applicable to processing of sowing material [1,2]. In order to ensure a high seed performance, various methods of processing are used, including chemical preparations, such as seed inoculation by chemical material and growth regulators, as well as physical factors, including laser light and magnetic fields [3][4][5][6][7][8].…”

Role of He-Ne laser pre-treatment in protecting Zea mays against the deleterious effects of ultraviolet radiations

“…The effect of He-Ne laser on plants has recently received attention this led to various investigations (Qiu et al, 2010(Qiu et al, & 2013Podleśny et al, 2012;Abu-Elsaoud and Tuleukhanov, 2013;Jamil et al, 2013;Jia and Duan, 2013;Abu-Elsaoud, 2013& 2015 . Nevertheless, these previous investigations have not focused on the damage repair role of laser on photosynthesis under ultraviolet radiations stress.…”

“…He-Ne l aser could signific antl y all eviat e UV-B damag e in winter wheat, t he protei n expression profiles of wi nt er w heat all eviated by He-N e las ers (Jia and Duan, 2013). The gene expression of s ever al prot eins e.g.…”

Bioestimulación láser en semillas y plantas

“…Los bioefectos debidos a la irradiación láser de baja intensidad (ILBI) en semillas presiembra y en plántulas o plantas durante su desarrollo (i.e., aplicación de energía fotónica en longitudes de onda y dosis específicas que producen un macro o micro efecto) han sido confirmados por numerosos estudios usando semillas de diversos cultivos (Paleg & Aspinall 1970, Govil et al 1983, Koper et al 1999, Katanska et al 2003, Hernández-Aguilar et al 2006, Drozd & Szajsner 2007, Podleśny 2007, Muszyñski & Gladyszewska 2008, Aladjadjiyan & Kakanakova 2008, Osman et al 2009, Aladjadjiyan 2012, Hoseini et al 2013, El-Kereti et al 2013, Jia & Duan 2013, Dobrowolski et al 1997, Śliwka 2014, Srećković et al 2014, Kouchebagh et al 2014, Gao et al 2014. A partir de la llegada de los láseres en los años 60´s (Nasim & Jamil 2014), se inició su aplicación en sistemas biológicos (Bessis et al 1962), incursionando al sector agrícola como elemento bioestimulador (BE) de plantas y semillas con el láser de rubí (Wilde et al 1969), y posterior a su descubrimiento también han sido aplicados los láseres de He-Ne (Helio-Neón), Ar (Argón), Neodimio-YAG (Nd-YAG), dióxido de carbono (CO 2 ) y diodos (en distintas longitudes de onda -λ), (Paleg & Aspinall 1977, Govil et al 1985,1991, Koper et al 1996, Rong et al 2001, Chen & Wang 2004, Chen & Sun 2006, Qiu et al 2008a, Chen 2008, Aladjadjiyan 2007a, 2007b…”