Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella Enteritidis infection

Thirabunyanon, Mongkol; Thongwittaya, Narin

doi:10.1016/j.rvsc.2011.08.008

Cited by 81 publications

(56 citation statements)

References 33 publications

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“…SEM was performed according to the method of Thirabunyanon and Thongwittaya (2012) with modifications. S. typhi ATCC 5784 was grown for 6 h at 37°C in NB until reaching the logarithmic phase.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Arbsuwan

Payoungkiattikun

Sirithorn

et al. 2017

CyTA - Journal of Food

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In this study, Bacillus licheniformis BFP011 has been cultivated for producing antimicrobial compounds, which were concentrated from the crude supernatant and purified using thin-layer chromatography. The antimicrobial activity was investigated in the presence of several organic solvents and detergents, and inhibiting effects to various Gram-negative and Gram-positive human pathogenic and food spoilage bacteria were observed. Three bands (F4, F5 and F6) showing antimicrobial activity against Salmonella typhi ATCC 5784 were subjected to reversed-phase high-performance liquid chromatography purification. Two peaks of fraction F5, F5-P3 and F5-P4, showed 100% inhibition against S. typhi ATCC 5784. The contained mixture of antimicrobial compounds consists of macrolactins and amicoumacins, which induced the collapse and breaking of S. typhi ATCC 5784 cell membranes in a time-dependent manner. Taken collectively, the results indicate that these compounds may represent promising candidates for the development of food preservative agents of natural origin, as well as novel antimicrobial drug candidates against multiresistant bacterial strains.Purificación y caracterización de macrolactinas y amicoumacinas procedentes de Bacillus licheniformis BFP011: una nueva fuente de sustancias antimicrobianas para los alimentos RESUMEN En el presente estudio, se cultivó Bacillus licheniformis BFP011 para producir compuestos antimicrobianos; estos fueron concentrados del sobrenadante crudo y purificado empleando cromatografía en capa fina (CCF). En presencia de varios solventes y detergentes orgánicos se analizó la actividad antimicrobiana, observándose, además, los efectos inhibidores de varias bacterias gram-negativas y gram-positivas, patógenas para los seres humanos y causantes del deterioro de los alimentos. Mediante RP-HPLC se purificaron tres franjas (F4, F5 y F6) que mostraron actividad antimicrobiana contra la Salmonella typhi ATCC 5784, comprobándose que los dos picos de la fracción F5 (F5-P3 y F5-P4) provocan una inhibición del 100% contra la S. typhi ATCC 5784. La mezcla de compuestos antimicrobianos contiene macrolactinas y amicoumacinas; estas produjeron el colapso y el rompimiento de las membranas celulares de S. typhi ATCC 5784 a medida que fue transcurriendo el tiempo. Considerados en su conjunto, estos resultados indican que es posible que dichos compuestos sean sustancias prometedoras para la creación de agentes conservantes de alimentos de origen natural, y para el desarrollo de medicamentos antimicrobianas novedosas contra cepas bacterianas multirresistentes. ARTICLE HISTORY

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Section: Scanning Electron Microscopymentioning

confidence: 99%

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Arbsuwan

Payoungkiattikun

Sirithorn

et al. 2017

CyTA - Journal of Food

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“…Bacteriocins are commonly defined as genetically encoded substances of a proteinaceous nature produced by virtually all bacteria and are active against various, most closely-related, microorganisms [6]. This positions them as a very appealing alternative to antibiotics and chemical stressors, especially in an age when alternative bacterial infection therapies are being widely investigated [7].…”

Section: Introductionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

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This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.

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“…Rehabilitation of the intestinal microbiota can have a positive effect on intestinal health and bird performance. Probiotics have been shown to suppress colonization of the intestine with pathogenic bacteria such as Brachyspira pilosicoli (28), Clostridium perfringens (41), Campylobacter jejuni (43), and Salmonella Enteritidis (57).…”

Section: Discussionmentioning

confidence: 99%

Can Bacteriotherapy Using Commercially Available Probiotics, Prebiotics, and Organic Acids Ameliorate the Symptoms Associated With Runting-Stunting Syndrome in Broiler Chickens?

et al. 2015

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Runting-stunting syndrome (RSS) in poultry has been known for more than 40 years, but the precise etiology remains unknown and a licensed vaccine is consequently not currently available. In order to mitigate the symptoms associated with RSS, a series of experiments was performed to investigate whether a combined bacteriotherapeutic treatment consisting of probiotics, prebiotics, and organic acids could influence the outcome of this disease. Initially two groups of commercial broiler chickens were either left uninoculated or inoculated with filtrate from homogenized intestines of RSS-affected broiler chickens. One group from each of these two challenge groups was treated, with a bacteriotherapeutic regimen. After 12 days chickens were euthanatized, the body weight was measured, and duodenal lesions were enumerated. Five consecutive broiler chicken flocks were then raised either on litter from RSS-affected birds or on fresh wood shavings. Treatment had no beneficial effect on the number and severity of intestinal lesions. There appeared to be a significant build-up of RSS agent(s) in poultry litter, with each consecutive flock placement, independent of bacteriotherapeutic treatment, as more individuals exhibited intestinal lesions on built-up litter in RSS-affected houses (28.9% vs. 44%). While treatment did not appear to consistently reduce intestinal lesions, it did significantly improve the mean body weights (P<0.05) and uniformity of 12-day-old chickens placed on reused litter in houses in which RSS-infected birds were previously raised. A combination of litter management and bacteriotherapy may be needed to ameliorate the adverse effects of RSS on intestinal health and body weight in broiler chickens.

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Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella Enteritidis infection

Cited by 81 publications

References 33 publications

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Can Bacteriotherapy Using Commercially Available Probiotics, Prebiotics, and Organic Acids Ameliorate the Symptoms Associated With Runting-Stunting Syndrome in Broiler Chickens?

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Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella Enteritidis infection

Cited by 81 publications

References 33 publications

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Can Bacteriotherapy Using Commercially Available Probiotics, Prebiotics, and Organic Acids Ameliorate the Symptoms Associated With Runting-Stunting Syndrome in Broiler Chickens?

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Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895