Protection against cisplatin-induced nephrotoxicity in mice by Curcuma comosa Roxb. ethanol extract

Abstract

The protective effect of an ethanol extract of Curcuma comosa against cisplatin-induced renal toxicity in mice was studied. Adult male mice were pretreated for 4 days with the ethanol extract of C. comosa [100-200 mg/kg body weight (BW), orally (p.o.)] before injection of cisplatin (12.5 mg/kg BW, intraperitoneally (i.p.)). Five days later the mice were killed, and blood samples were collected to determine blood urea nitrogen (BUN) and plasma creatinine levels. Kidneys were examined histopathologically and levels of lipid peroxidation, gluthathione (GSH) content, and superoxide dismutase (SOD), gluthathione peroxidase (GPx), and catalase (CAT) activities were determined. Histological examinations revealed degenerative changes and tubular necrosis in mice treated with cisplatin, which were improved by pretreatment with C. comosa ethanol extract. Cisplatin raised BUN, creatinine, and kidney lipid peroxidation levels, and lowered kidney GSH content and levels of GPx, SOD, and CAT activities, all of which (except SOD and CAT) could be restored to normal values by pretreatment with 200 mg/kg BW of C. comosa ethanol extract. In addition, the ethanol extract of C. comosa and its isolated diarylheptanoid compound also exhibited radical scavenging activities. The results suggest that the ethanol extract of C. comosa exhibits effective protection against cisplatin-induced nephrotoxicity mediated through its antioxidant activity.

Lipid-Lowering and Antioxidative Activities of Aqueous Extracts ofOcimum sanctumL. Leaves in Rats Fed with a High-Cholesterol Diet

“…Free radical-induced lipid peroxidation or oxidative stress has been shown to participate in the pathogenesis of several diseases [29, 30]. Hypercholesterolemia induces not only atherosclerosis but also produces a lot of free radicals in blood and tissues [30, 31].…”

Induction of apoptosis in murine leukemia by diarylheptanoids from Curcuma comosa Roxb.

“…In the course of our investigation on the chemoprevention of anti-oxidant compounds from natural products in several cancer cell lines, Compound-092 which has previously been shown to have antioxidant activity comparable to vitamin C (Jariyawat et al 2009), exhibit a highly toxic and selective to murine P388 leukemic cells. We, therefore, further Compound-091 which bears only one hydroxyl group at 4′-position (Fig.…”

“…Their anti-inflammatory activity in PMA-stimulated human mononuclear cells have been shown to inhibit IκB kinase which further suppresses the activation of NF-κB and the secretion of proinflammatory cytokines (Sodsai et al 2007). In addition, some diarylheptanoids from C. comosa exhibit anti-oxidant function through a direct scavenging effect (Jariyawat et al 2009). However, under certain conditions, a number of polyphenolic antioxidants such as tocopherol, curcumin, catechin, quercetin, caffeic acid, and resveratrol can switch to pro-oxidants, inducing DNA strand damage (Hadi et al 2007(Hadi et al , 2010Kong et al 2009;Sakihama et al 2002;Shamim et al 2008;Zheng et al 2006).…”

Protection of centrilobular necrosis by Curcuma comosa Roxb. in carbon tetrachloride-induced mice liver injury

“…It is likely that the protective effect of the Curcuma comosa may not mediate through a direct interaction with the reactive metabolite of CCl 4 since it possessed low radical scavenging activity (Jariyawat et al, 2009). Furthermore, the extract also took several hours before it exerted the protective effect.…”