Protection Against Free Radicals (UVB Irradiation) of a Water-Soluble Enzymatic Extract from Rice Bran. Study Using Human Keratinocyte Monolayer and Reconstructed Human Epidermis

Therapeutic properties of rice constituents and derivatives (Oryza sativa L.): A review update

“…A bran extract subjected to enzymatic fermentation has been evaluated on cultivated keratinocytes and in vitro-reconstructed skin, serving as a protective screen against UV-B radiation (Santa-Maria, Revilla et al 2010). Similarly, another bran fermented extract, obtained from Lactobacillus rhamnosus and Saccharomyces cerevisiae, has protected fibroblasts from in vitro exposure to UV-B rays (Seo, Jung et al 2010).…”

Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

“…ISO 24443:2012 is an in vitro version of the UVA-PF [101]. Other substitutes for in vivo testing have been suggested with the use of cells and regenerated tissues [117,118] or with the use of animals [101,[119][120][121]. A recent article in the study by Aparecida-Figueiredo et al [98] assessed the protective efficacy of sunscreen products using a neonatal cell culture of human dermal fibroblasts by evaluating calcineurin depletion induced by UVA radiation.…”

Mechanisms of Uv Stabilization

“…Radical deactivation is also considered to be a part of the mechanism of other UV stabilizers, such as • ferulic acid [19][20][21] • phenoxy antioxidants 22 • flavonoids 23 • vitamin C and E and β-carotene 24 • some natural extracts 25 • carbon black [26][27][28] • copper stearate 29,30 Ferulic acid (4-hydroxy-3-methoxycinnamic acid) acts as scavenger of hydroxyl and peroxyl radicals and superoxide anions, inhibiting propagation of lipid peroxydation chain reaction. 19 It also reduces the UVB-induced erythema, because of its high effectiveness in scavenging nitric oxide, and provides a high degree of skin protection acting as UVabsorber screen.…”

“…19 Extract from rice bran has substantial concentrations of ferulic acid and tocopherols (vitamin E), which are both radical scavengers. 20 Ferulic acid is used as a photoprotective ingredient in many skin lotions and sunscreens. 21 Phenol-type antioxidants are known as radical scavengers.…”