Protection against human schistosome cercariae

Pellegrino, J.

doi:10.1016/0014-4894(67)90073-2

Cited by 37 publications

(11 citation statements)

References 67 publications

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“…Several reports describe other activities from copaiba oils, obtained from different Copaifera species, such as bactericidal (Maruzzela and Sicurella, 1960), antihelmintic (Pellegrino, 1967;Gilbert et al, 1972), analgesic (Fernandes and Pereira, 1989), antiinflammatory (Basile et al, 1988;Fernandes et al, 1992), gastroprotective (Paiva et al, 1998) and trypanocidal (Cascon et al, 1998).…”

Section: Discussionmentioning

confidence: 96%

In vivo and in vitro studies on the anticancer activity of Copaifera multijuga hayne and its fractions

Lima¹,

Veiga

Christo

et al. 2003

Phytotherapy Research

118

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Copaiba oil resin (COR) obtained from Copaifera multijuga Hayne has been used in popular medicine as an antinflammatory and for the treatment of bronchitis, ulcers and cancer. The aim of this study was to evaluate the action of COR and its fractions on the inhibition of lung metastasis and tumour growth induced by B16F10 melanoma cells in mice and cytotoxicity in vitro using Trypan Blue exclusion method and MTT conversion. Mice which have received subcutaneously B16F10 cells developed a solid tumour that reached a peak at 17 days. Together with the increase in tumour growth we also observed an increase in the number of lung nodules. There was a positive correlation between the in vitro cytotoxic assay and in vivo antitumour activity. The oral administration of COR (at 2 g/Kg in the days 3, 5, 7, 10, 12 and 14 after inoculation of tumoral cells) reduced tumour growth by 58% and tumour weight by 76%. At the same dose COR reduced the number of lung nodules by 47.1%. In vitro experiments showed that COR incubated with the melanoma cell line reduced cell viability in a concentration and time-dependent manner. Diterpenic and sesquiterpenic fractions or reconstituted oil induced cytotoxicity. Our results shows that COR and its fractions have tumouricidal activity in the melanoma cell line in both models in vivo and in vitro.

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Section: Discussionmentioning

confidence: 96%

In vivo and in vitro studies on the anticancer activity of Copaifera multijuga hayne and its fractions

Lima¹,

Veiga

Christo

et al. 2003

Phytotherapy Research

118

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“…The oleoresin is a natural solution of diterpene acids in an essential oil composed mainly of sesquiterpenes and has been reported as being bactericidal (Maruzzela and Sicurella, 1960;Opdyke, 1976;Tincusi et al, 2002), antihelminthic (Pellegrino, 1967;Gilbert et al, 1972), analgesic (Fernandes and Pereira, 1989), anti-inflammatory (Basile et al, 1988;Fernandes et al, 1992;Veiga-Junior et al, 2001) and gastro-protective (Paiva et al, 1998) as well as showing antitumor (Ohsaki et al, 1994;Lima et al, 1998) and trypanocidal activity. However, in several of these evaluations commercial copaiba oleoresins were used, the chemical composition of which was either not given or only partially described.…”

Section: Introductionmentioning

confidence: 99%

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

et al. 2005

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We characterized the chemical constituents of Copaifera duckei oleoresin and used dermal application to Wistar rats to evaluated its possible mutagenic and cytotoxic activities on peripheral blood reticulocytes and bone marrow cells. Chemical characterization of the oleoresin revealed the presence of sesquiterpene hydrocarbons, an unidentified neutral diterpene and diterpene acids. To evaluate mutagenicity evaluation the rats were treated with 10, 25 and 50% of the LD 50 dose of the oleoresin for three consecutive days and peripheral blood collected after 0, 24, 48 and 72 h for micronucleus analysis. The rats were humanly sacrificed 24 hours after the last treatment and chromosome preparations made using standard techniques. At the three concentrations and the three time intervals tested we found that there were no statistically significant differences in either the mean number of micronucleated reticulocytes (MNRETs) or the number of chromosomal aberrations as to the negative control. However, at 25 and 50% of the LD 50 dose of the oleoresin there was a significant decrease in the mitotic index (MI) as compared to the negative control. Under our experimental conditions, C. duckei V11 oleoresin produced no mutagenic effects on bone marrow cells or in peripheral reticulocytes as assessed by chromosome aberrations and the micronucleus test respectively, but showed cytotoxic activity at high doses.

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“…In the past, there were some attempts amongst researchers to develop a formulation that either prevents cercarial recognition or penetration of the human skin. Predominantly for human schistosomes, a series of substances was reported to have anti-penetrant or repellent potential, respectively (Pellegrino 1967). For example, the insecticide dimethylphthalate (MacFarlane 1949), its derivative dibutylphthalate (Yang et al 1965) and the molluscicide niclosamide (Cherfas 1989;AbuElyazeed et al 1993) were reported to inhibit cercarial skin penetration with varying efficiency.…”

Section: Introductionmentioning

confidence: 99%

Cream formulations protecting against cercarial dermatitis by Trichobilharzia

2007

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Dermatitis caused by penetrating bird schistosome cercariae is an emerging global public health problem. Infections may be prevented by the use of topical formulations that inhibit cercarial skin penetration. We evaluated nine water resistant formulations by exposing treated arms of volunteers to Trichobilharzia szidati cercariae. Six formulations protected from cercarial invasion. However, after immersion of the treated skin in water (2 x 20 min), only two formulations offered full protection: (1) Safe Sea, a cream protecting against jelly fish, (2) niclosamide in water resistant sun protecting cream formulations at concentrations as low as 0.05%. In an in vitro system Safe Sea and a 0.1% niclosamide formulation caused a high damage rate in T. szidati (92% and 99% after 5 min; only niclosamide with lethal effect) but not in Schistosoma mansoni (1% and 72%; both formulations with lethal effect). However, a 1% niclosamide formulation damaged S. mansoni sufficiently (100% after 5 min) and might offer full penetration protection.

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Protection against human schistosome cercariae

Cited by 37 publications

References 67 publications

In vivo and in vitro studies on the anticancer activity of Copaifera multijuga hayne and its fractions

In vivo and in vitro studies on the anticancer activity of Copaifera multijuga hayne and its fractions

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

Cream formulations protecting against cercarial dermatitis by Trichobilharzia

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