Protection against influenza H7N9 virus challenge with a recombinant NP–M1–HSP60 protein vaccine construct in BALB/c mice

Yang, Peng; Wang, Wenjuan; Gu, Huiying; Li, Zhiwei; Zhang, Keming; Wang, Zhouhai; Li, Ruisheng; Duan, Yueqiang; Zhang, Shaogeng; Wang, Xiliang

doi:10.1016/j.antiviral.2014.08.008

Cited by 14 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As of March 7, 2015, the IEDB reported 167 T cell epitopes and four linear B cell epitopes of the M1 protein in the human host. Previous findings suggest that the three peptides identified in the present study have not been reported, although some substrings of peptide fragments have been reported to induce a CD4+ and CD8+ T cell response (4,19,56). The literature reports that the substrings of these peptides bind specifically to only one or two HLA alleles, whereas the current study reports that these peptides are capable of binding to a large number of class I and II HLA alleles.…”

Section: Discussioncontrasting

confidence: 53%

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Lohia

Baranwal

2015

Viral Immunology

View full text Add to dashboard Cite

Cell mediated immune response plays a key role in combating viral infection and thus identification of new vaccine targets manifesting T cell mediated response may serve as an ideal approach for influenza vaccine. The present study involves the application of an immunoinformatics-based consensus approach for epitope prediction (three epitope prediction tools each for CD4+ and CD8+ T cell epitopes) and molecular docking to identify peptide sequences containing T cell epitopes using the conserved sequences from all the Matrix 1 protein sequences of H1N1 virus available until April 2015. Three peptides comprising CD4+ and CD8+ T cell epitopes were obtained, which were not exactly reported in earlier studies. Population coverage study of these multiepitope peptides revealed that they are capable of inducing a potent immune response belonging to individuals from different populations and ethnicity distributed around the globe. Conservation study with other subtypes of influenza virus infecting humans (H2N2, H5N1, H7N9, and H3N2) revealed that these three peptides were conserved (>90%), with 100% identity in most of these strains. Hence, these peptides can impart immunity against H1N1 as well as other subtypes of influenza virus. A molecular docking study of the predicted peptides with class I and II human leukocyte antigen (HLA) molecules has shown that the majority of them have comparable binding energies to that of native peptides. Hence, these peptides from Matrix 1 protein of H1N1 appear to be promising candidates for universal vaccine design.

show abstract

Section: Discussioncontrasting

confidence: 53%

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Lohia

Baranwal

2015

Viral Immunology

View full text Add to dashboard Cite

show abstract

“…Ben et al [ 21 ] found that immunization of mice with Strongyloides ratti Hsp60 (srHSP60) led to reduced numbers of migrating larvae, parasitic adults and larval output of S. ratti , and protected mice against nematode challenge infection. Moreover, immunization of female BALB/c mice with recombinant protein NP-M1-HSP60 induced immune responses, and protected mice from lethal influenza H7N9 virus challenge and significantly inhibited viral replication [ 22 ]. In our previous studies [ 7 ], 66 kDa proteins from H. longicornis had immunogenic properties, which might be Hsp60 verified later by LC-MS/MS.…”

mentioning

confidence: 99%

Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)

Zhang

Cui

Zhang³

et al. 2015

Korean J Parasitol

View full text Add to dashboard Cite

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

show abstract

“…constructed recombinant H1N1 NP and M1 proteins with HSP60, and then immunized mice with an oil-in-water adjuvant through the intranasal route. This combination induced balanced IgG1 and IgG2a levels, high mucosal and cellular responses, and complete protection against lethal H7N9 challenge ( 262 ). In a pre-print article, prime immunization with recombinant H1N1 NP and M1 DNA vaccine containing calreticulin, another HSP, followed by boosting with live attenuated influenza vaccine in mice was shown to confer better protection than commercial SVV against lethal H1N1 challenge ( 263 ).…”

Section: Recombinant Influenza Vaccinesmentioning

confidence: 99%

Recent Progress in Recombinant Influenza Vaccine Development Toward Heterosubtypic Immune Response

2022

View full text Add to dashboard Cite

Flu, a viral infection caused by the influenza virus, is still a global public health concern with potential to cause seasonal epidemics and pandemics. Vaccination is considered the most effective protective strategy against the infection. However, given the high plasticity of the virus and the suboptimal immunogenicity of existing influenza vaccines, scientists are moving toward the development of universal vaccines. An important property of universal vaccines is their ability to induce heterosubtypic immunity, i.e., a wide immune response coverage toward different influenza subtypes. With the increasing number of studies and mounting evidence on the safety and efficacy of recombinant influenza vaccines (RIVs), they have been proposed as promising platforms for the development of universal vaccines. This review highlights the current progress and advances in the development of RIVs in the context of heterosubtypic immunity induction toward universal vaccine production. In particular, this review discussed existing knowledge on influenza and vaccine development, current hemagglutinin-based RIVs in the market and in the pipeline, other potential vaccine targets for RIVs (neuraminidase, matrix 1 and 2, nucleoprotein, polymerase acidic, and basic 1 and 2 antigens), and deantigenization process. This review also provided discussion points and future perspectives in looking at RIVs as potential universal vaccine candidates for influenza.

show abstract

Protection against influenza H7N9 virus challenge with a recombinant NP–M1–HSP60 protein vaccine construct in BALB/c mice

Cited by 14 publications

References 34 publications

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)

Recent Progress in Recombinant Influenza Vaccine Development Toward Heterosubtypic Immune Response

Contact Info

Product

Resources

About

Protection against influenza H7N9 virus challenge with a recombinant NP–M1–HSP60 protein vaccine construct in BALB/c mice

Cited by 14 publications

References 34 publications

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Identification of Conserved Peptides Comprising Multiple T Cell Epitopes of Matrix 1 Protein in H1N1 Influenza Virus

Screening and Identification of Antigenic Proteins from the Hard Tick &lt;i&gt;Dermacentor silvarum&lt;/i&gt; (Acari: Ixodidae)

Recent Progress in Recombinant Influenza Vaccine Development Toward Heterosubtypic Immune Response

Contact Info

Product

Resources

About

Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)