Protection against influenza virus challenge by topical application of influenza DNA vaccine

Watabe, Setsuko; Xin, Ke-Qin; Ihata, Atsushi; Liu, Lijuan; Honsho, Akiko; Aoki, Ichiro; Hamajima, Kenji; Wahrén, Britta; Okuda, Kenji

doi:10.1016/s0264-410x(01)00194-3

Cited by 81 publications

(52 citation statements)

References 44 publications

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“…Previously, we demonstrated that DNA-based vaccination results in the induction of virus-specific immunity to several viral pathogens, including HIV-1 (3,47,48,53,64,76). Furthermore, we recently established a system to express HIV-1 genes by inserting them into a deleted region of the attenuated vaccinia virus strain, DIs (25,30,71).…”

Section: Discussionmentioning

confidence: 99%

A Consecutive Priming-Boosting Vaccination of Mice with Simian Immunodeficiency Virus (SIV)gag/polDNA and Recombinant Vaccinia Virus Strain DIs Elicits Effective Anti-SIV Immunity

Someya

Xin

Matsuo

et al. 2004

J Virol

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To evaluate immunity induced by a novel DNA prime-boost regimen, we constructed a DNA plasmid encoding the gag and pol genes from simian immunodeficiency virus (SIV) (SIVgag/pol DNA), in addition to a replication-deficient vaccinia virus strain DIs recombinant expressing SIV gag and pol genes (rDIsSIVgag/pol). In mice, priming with SIVgag/pol DNA, followed by rDIsSIVgag/pol induced an SIV-specific lymphoproliferative response that was mediated by a CD4؉ -T-lymphocyte subset. Immunization with either vaccine alone was insufficient to induce high levels of proliferation or Th1 responses in the animals. The prime-boost regimen also induced SIV Gag-specific cellular responses based on gamma interferon secretion, as well as cytotoxic-T-lymphocyte responses. Thus, the regimen of DNA priming and recombinant DIs boosting induced Th1-type cell-mediated immunity, which was associated with resistance to viral challenge with wild-type vaccinia virus expressing SIVgag/pol, suggesting that this new regimen may hold promise as a safe and effective vaccine against human immunodeficiency virus type 1.

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Section: Discussionmentioning

confidence: 99%

A Consecutive Priming-Boosting Vaccination of Mice with Simian Immunodeficiency Virus (SIV)gag/polDNA and Recombinant Vaccinia Virus Strain DIs Elicits Effective Anti-SIV Immunity

Someya

Xin

Matsuo

et al. 2004

J Virol

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“…Adhesive tapes were used to strip the stratum corneum again and again in some researches, then the viable epidermis layer was exposed in order to inoculate DNA vaccine. 30) Compared to adhesive tapes, microneedle penetration is a minimal invasive approach, which makes only a little hurt to the skin. The micropores to be penetrated by the microneedles is temporary and has normal physiological function of the skin.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B DNA Vaccine-Polycation Nano-Complexes Enhancing Immune Response by Percutaneous Administration with Microneedle

Yin

Liang

Xing

et al. 2013

Biological & Pharmaceutical Bulletin

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Percutaneous immune method is becoming an attractive alternative for DNA vaccine as a lot of antigen presenting cells are existed in the viable epidermis. However, due to the barrier function of stratum corneum, it would be hard for DNA vaccine to reach the viable epidermis of the skin. In order to deliver the DNA vaccine successfully cross the stratum corneum, pentagram silicon microneedle array was prepared in this study, and fluorescently labeled nanoparticle was taken as the model to observe the situation inside the skin processed by microneedle. Via microneedle nanoparticles could enter the skin through the micro-channel (diameter about 20-30 µm) and its amount is greatly larger than that enter though the hair follicle of intact skin. A new type of gene vector Pluronic P123-modified polyethyleneimine (P123-PEI) was synthesized by high molecular weight polyethylenimine and Pluronic P123 with the molar ratio of 1 : 1 to take the advantage of P123-PEI as low cytotoxicity and high transfection efficiency. Mice were immunized percutaneously with Hepatitis B DNA vaccine/P123-PEI nano-complexes by microneedle. The humoral and cellular immunity generated in percutaneously immunized mice through microneedle array by Hepatitis B DNA vaccine/P123-PEI nano-complex was significantly higher than that of DNA vaccine intramuscular administration.Key words hepatitis B DNA vaccine; microneedle; percutaneous immune; nano-complex; Pluronic P123-modified polyethyleneimine DNA vaccine that the eukaryon expression plasmid encoded by antigen gene was inoculated in vivo and can express corresponding antigen to stimulate the organism to generate immune response to the antigen and protective immunity was the third generation vaccine on the basis of gene therapy and transgenic technology, which marked a vaccine revolution and has a wide application prospect. 1) However, DNA vaccines had its own disadvantage. Numerous experiments, particularly big animal experiments and human clinical trials, demonstrated that currently DNA vaccine had the disadvantages such as large dose, low bioavailability, great variation in immunity effect among individuals and low level of humoral immunity induced etc. The disadvantage of DNA vaccine enormously affected the research progress and the wide clinical application.2) According to in vivo immune mechanism of DNA vaccine, antigen presenting cells (APCs) like phagocytes and dendritic cells was playing an important role in the process of transfection, expression and presentation of DNA vaccine. 3,4)How to improve the cell transfection efficiency of DNA vaccine, particularly the transfection efficiency of APCs, becomes one of the key approaches for improvement of immunity effect of DNA vaccines. Currently, the dosage forms of DNA vaccine are mainly water solution or lyophilized powder injection and intramuscular injection is the most common route of immune administration. DNA vaccine is usually consisted of 2-10 kbp plasmid DNA and his molecular weight reached million with a strong hydrophilicity and low oi...

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“…The pH-sensitive liposomes are being explored as promising vehicles for delivery of therapeutic and prophylactic vaccines (Chang et al, 2001;Watabe et al, 2001). These liposomes are employed as effective means for delivery of small sized peptides, producing efficient immune response and reducing their toxicity (Tachibana et al, 1998).…”

Section: The Ph-sensitive Liposomes In Vaccine Deliverymentioning

confidence: 99%

A review of mechanistic insight and application of pH-sensitive liposomes in drug delivery

2014

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The pH-sensitive liposomes have been extensively used as an alternative to conventional liposomes in effective intracellular delivery of therapeutics/antigen/DNA/diagnostics to various compartments of the target cell. Such liposomes are destabilized under acidic conditions of the endocytotic pathway as they usually contain pH-sensitive lipid components. Therefore, the encapsulated content is delivered into the intracellular bio-environment through destabilization or its fusion with the endosomal membrane. The therapeutic efficacy of pHsensitive liposomes enables them as biomaterial with commercial utility especially in cancer treatment. In addition, targeting ligands including antibodies can be anchored on the surface of pH-sensitive liposomes to target specific cell surface receptors/antigen present on tumor cells. These vesicles have also been widely explored for antigen delivery and serve as immunological adjuvant to enhance the immune response to antigens. The present review deals with recent research updates on application of pH-sensitive liposomes in chemotherapy/ diagnostics/antigen/gene delivery etc.

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Protection against influenza virus challenge by topical application of influenza DNA vaccine

Cited by 81 publications

References 44 publications

A Consecutive Priming-Boosting Vaccination of Mice with Simian Immunodeficiency Virus (SIV)gag/polDNA and Recombinant Vaccinia Virus Strain DIs Elicits Effective Anti-SIV Immunity

A Consecutive Priming-Boosting Vaccination of Mice with Simian Immunodeficiency Virus (SIV)gag/polDNA and Recombinant Vaccinia Virus Strain DIs Elicits Effective Anti-SIV Immunity

Hepatitis B DNA Vaccine-Polycation Nano-Complexes Enhancing Immune Response by Percutaneous Administration with Microneedle

A review of mechanistic insight and application of pH-sensitive liposomes in drug delivery

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