Protection against UVB-Induced Photoaging by Nypa fruticans via Inhibition of MAPK/AP-1/MMP-1 Signaling

Abstract

Ultraviolet B (UVB) irradiation is major causative factor in skin aging. The aim of the present study was to investigate the protective effect of a 50% ethanol extract from Nypa fruticans (NF50E) against UVB-induced skin aging. The results indicated that NF50E exerted potent antioxidant activity (IC50 = 17.55 ± 1.63 and 10.78 ± 0.63 μg/mL for DPPH and ABTS-radical scavenging activity, respectively) in a dose-dependent manner. High-performance liquid chromatography revealed that pengxianencin A, protocatechuic acid, catechin, chlorogenic acid, epicatechin, and kaempferol were components of the extract. In addition, the extract exhibited elastase inhibitory activity (IC50 = 17.96 ± 0.39 μg/mL). NF50E protected against UVB-induced HaCaT cell death and strongly suppressed UVB-stimulated cellular reactive oxygen species generation without cellular toxicity. Moreover, topical application of NF50E mitigated UVB-induced photoaging lesions including skin erythema and skin thickness in BALB/C mice. NF50E treatment inhibited UVB-induced collagen degradation as well as MMP-1 and IL-1β expressions and significantly stimulated SIRT1 expression. Furthermore, the extract treatment markedly suppressed the activation of NF-κB and AP-1 (p-c-Jun) by deactivating the p38 and JNK proteins. Taken together, current data suggest that NF50E exhibits potent antioxidant potential and protection against photoaging by attenuating MMP-1 activity and collagen degradation possibly through the downregulation of MAPK/NF-κB/AP-1 signaling and SIRT1 activation.

Evaluation of Gallic Acid-Coated Gold Nanoparticles as an Anti-Aging Ingredient

“…Phenolic acids can act as free radical scavengers either by donating a hydrogen atom or acting as electron donors [12,13]. Phytochemicals, including (-)-epigallocatechin-3-O-gallate (EGCG), resveratrol, ferulic acid, and protocatechuic acid show potential as Pharmaceuticals 2021, 14, 1071 2 of 14 anti-skin-aging treatments for their antioxidant properties and inhibition of MMP-1 in in vitro assays and for anti-wrinkle properties as shown in in vivo skin tests [14][15][16]. Strategies for reducing the generation of intracellular ROS have been developed to prevent MMP-1 upregulation and protect skin from aging.…”

Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1

“…Furhermore, caffeine content in RE may contribute to decrease ROS production through enchance superoxide dismutase 2 (SOD2) activity. (29) RE contains several bioactive compound such as caffeine, CGA, and phenolic acid. High CGA content may contribute to oxidative stress.…”

Panax ginseng C. A. Meyer Phenolic Acid Extract Alleviates Ultraviolet B-Irradiation-Induced Photoaging in a Hairless Mouse Skin Photodamage Model

“…Entry of these cytokines into skin tissues leads to inflammatory damage and elevation of expression of various matrix metalloproteinases (MMPs) that degrade collagen fibers and accelerate skin photoaging [11][12][13][14]. MMPs are important enzymes with key roles in collagen-degradative processes that occur in the ECM, which is located within connective tissue [15,16]. Researchers are currently devising strategies that reduce inflammation and MMPs activities toward preventing and treating skin photodamage after UVB exposure.…”