Protection effect of gelatin-xylooligosaccharides Maillard reaction products on spray-dried Limosilactobacillus fermentum and possible action mechanism

Li, Huiyu; Peng, Fei; Peng, Zhen; Liu, Zhanggen; Huang, Tao; Xiong, Tao

doi:10.1016/j.fbio.2023.103251

Cited by 3 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study by Cheng et al (2022) demonstrated that the key enzymes associated with carbohydrate metabolism were impaired following the dehydration–drying process and that the injury to the membrane was reflected by the measurement of LDH and β-GAL activity [ 12 ]. Notably, HK, PK, LDH, and β-GAL play pivotal roles in carbohydrate metabolism pathways, particularly within the glycolytic pathways that provide energy for the improved growth of LAB strains [ 5 , 13 ]. From the perspective of energy generation and transformation view, β-GAL as glycoside hydrolase promotes the decomposition of polysaccharides into glucose or other monosaccharides [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Screening the Protective Agents Able to Improve the Survival of Lactic Acid Bacteria Strains Subjected to Spray Drying Using Several Key Enzymes Responsible for Carbohydrate Utilization

Liu,

Xie,

et al. 2024

Microorganisms

View full text Add to dashboard Cite

The aim of this study was to identify the most effective protectants for enhancing the viability of specific lactic acid bacteria (LAB) strains (Lactobacillus delbrueckii subsp. bulgaricus CICC 6097, Lactiplantibacillus plantarum CICC 21839, Lactobacillus acidophilus NCFM) by assessing their enzymatic activity when exposed to spray drying (inlet/outlet temperature: 135 °C/90 °C). Firstly, it was found that the live cell counts of the selected LAB cells from the 10% (w/v) recovered skim milk (RSM) group remained above 107 CFU/g after spray drying. Among all the three groups (1% w/v RSM group, 10% w/v RSM group, and control group), the two enzymes pyruvate kinase (PK) and lactate dehydrogenase (LDH) were more sensitive to spray drying than hexokinase (HK) and β-galactosidase (β-GAL). Next, transcriptome data of Lb. acidophilus NCFM showed that 10% (w/v) RSM improved the down-regulated expressions of genes encoding PK (pyk) and LDH (ldh) after spray drying compared to 1% (w/v) RSM. Finally, four composite protectants were created, each consisting of 10% (w/v) RSM plus a different additive—sodium glutamate (CP-A group), sucrose (CP-B group), trehalose (CP-C group), or a combination of sodium glutamate, sucrose, and trehalose (CP-D group)—to encapsulate Lb. acidophilus NCFM. It was observed that the viable counts of strain NCFM (8.56 log CFU/g) and enzymatic activity of PK and LDH in the CP-D group were best preserved compared to the other three groups. Therefore, our study suggested that measuring the LDH and PK activity could be used as a promising tool to screen the effective spray-dried protective agent for LAB cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Screening the Protective Agents Able to Improve the Survival of Lactic Acid Bacteria Strains Subjected to Spray Drying Using Several Key Enzymes Responsible for Carbohydrate Utilization

Liu,

Xie,

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Maillard-induced chia seed mucilage glycation-modified whey protein isolate conjugate: Insights from functional properties and untargeted metabolomics

Jiang,

Zheng,

Hou

et al. 2025

Food Bioscience

View full text Add to dashboard Cite

Protective Effects of Laminaria japonica Polysaccharide Composite Microcapsules on the Survival of Lactobacillus plantarum during Simulated Gastrointestinal Digestion and Heat Treatment

Guo,

Zhou,

Xie

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

To improve probiotics’ survivability during gastrointestinal digestion and heat treatment, Lactobacillus plantarum was microencapsulated by spray-drying using Laminaria japonica polysaccharide/sodium caseinate/gelatin (LJP/SC/GE) composites. Thermogravimetry and differential scanning calorimetry results revealed that the denaturation of LJP/SC/GE microcapsules requires higher thermal energy than that of SC/GE microcapsules, and the addition of LJP may improve thermal stability. Zeta potential measurements indicated that, at low pH of the gastric fluid, the negatively charged LJP attracted the positively charged SC/GE, helping to maintain an intact microstructure without disintegration. The encapsulation efficiency of L. plantarum-loaded LJP/SC/GE microcapsules reached about 93.4%, and the survival rate was 46.9% in simulated gastric fluid (SGF) for 2 h and 96.0% in simulated intestinal fluid (SIF) for 2 h. In vitro release experiments showed that the LJP/SC/GE microcapsules could protect the viability of L. plantarum in SGF and release probiotics slowly in SIF. The cell survival of LJP/SC/GE microcapsules was significantly improved during the heat treatment compared to SC/GE microcapsules and free cells. LJP/SC/GE microcapsules can increase the survival of L. plantarum by maintaining the lactate dehydrogenase and Na+-K+-ATPase activity. Overall, this study demonstrates the great potential of LJP/SC/GE microcapsules to protect and deliver probiotics in food and pharmaceutical systems.

show abstract

Protection effect of gelatin-xylooligosaccharides Maillard reaction products on spray-dried Limosilactobacillus fermentum and possible action mechanism

Cited by 3 publications

References 31 publications

Screening the Protective Agents Able to Improve the Survival of Lactic Acid Bacteria Strains Subjected to Spray Drying Using Several Key Enzymes Responsible for Carbohydrate Utilization

Screening the Protective Agents Able to Improve the Survival of Lactic Acid Bacteria Strains Subjected to Spray Drying Using Several Key Enzymes Responsible for Carbohydrate Utilization

Maillard-induced chia seed mucilage glycation-modified whey protein isolate conjugate: Insights from functional properties and untargeted metabolomics

Protective Effects of Laminaria japonica Polysaccharide Composite Microcapsules on the Survival of Lactobacillus plantarum during Simulated Gastrointestinal Digestion and Heat Treatment

Contact Info

Product

Resources

About