Protection Effect of Zhen‐Wu‐Tang on Adriamycin‐Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage

Abstract

Zhen-wu-tang (ZWT), a well-known formula in China, is widely used to treat chronic kidney diseases. However, very little information on ZWT's mechanism of action is currently available. In this study, we investigated the possible protective role and underlying mechanism of ZWT on nephrotic syndrome (NS) induced by Adriamycin (intravenous injection, 6.0 mg/kg) in rats using biochemical and histopathological approaches. ZWT decreased urine protein excretion and the serum levels of total cholesterol, triglycerides, blood urea nitrogen, and creatinine significantly in diseased rats. A decrease in plasma levels of total protein and albumin was also recorded in nephropathic rats. Pathological results show an improved pathological state and recovering glomerular structure in ZWT treatment groups. ZWT decreased renal IL-8 level but increased renal IL-4 level. In addition, rats subjected to ZWT exhibited less IgG deposition in glomerulus compared with model group. RT-PCR results showed that ZWT decreased the mRNA expression of NF-κB p65 and increased the mRNA expression of IκB. Furthermore, ZWT reduced the level of MDA and increased SOD activity. These results demonstrated that ZWT ameliorated Adriamycin-induced NS in rats possibly by inhibiting Adriamycin-induced inflammation damage, enhancing body's antioxidant capacity, thereby protecting glomerulus from injury.

Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy

“…In our study, massive proteinuria, pathological damage in renal tissue, podocyte foot process effacement, and downregulation of nephrin were observed in rats with adriamycin-induced nephropathy. These results were consistent with those of previously published reports [ 26 , 27 ]. In earlier studies, liver toxicity, nephrotoxicity, and infertility were considered the major side effects of the pharmacological constituents of Tripterygium wilfordii Hook F [ 28 , 29 ].…”

MiR‐145‐5p inhibits proliferation and inflammatory responses of RMC through regulating AKT/GSK pathway by targeting CXCL16

“…On day 28, all animals were euthanatized to collect their blood and obtain renal tissue samples, as our previously described (Liang et al, ). Five milliliters of blood from the abdominal aorta was collected after anesthetization, and then centrifuged at 3,500 rpm at 4°C for 15 min to obtain the serum for renal function analysis.…”

Triptolide Attenuates Inflammatory Response in Membranous Glomerulo-Nephritis Rat via Downregulation of NF-κB Signaling Pathway

“…At the end of experiment, animals were euthanized and blood samples and renal tissue samples were collected as previously described [18]. Blood were collected from abdominal aorta, followed by centrifugation at 3,500 rpm at 4°C for 15 minutes.…”