Protection from gamma-radiation insult to antioxidant defence and cellular DNA by POLY-MVA, a dietary supplement containing palladium-lipoic acid formulation

Menon, Aditya; Krishnan, C. V.; Nair, Cherupally Krishnan Krishnan

doi:10.1504/ijlr.2009.028892

Cited by 9 publications

(5 citation statements)

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“…Additionally, the radiation-induced DNA damage in these cells was reduced when poly-MVA was administered to animals exposed to a lethal dose of 8 Gy whole-body ␥-radiation [14]. Its administration, for seven days prior to whole body gamma radiation, significantly reduced the damage to cellular DNA in bone marrow and blood leukocytes and prevented the radiation-induced decrease of tissue antioxidant levels [13]. Administration of poly-MVA for 14 days before exposure to 6 Gy gamma radiation, from a Cs-137 source, resulted in normal blood viscosity and yield stress compared to a control group 14 days after exposure, reducing the damage induced by radiation exposure.…”

Section: Introductionmentioning

confidence: 99%

“…Toxicological studies indicated that the LD 50 of poly-MVA exceeded 5000 mg/kg, and no mutagenic effect of the combination was observed in the Ames test [12]. In a few recent studies, it showed significant radio-protective and mitigation effects [13][14][15][16]. It significantly reduced the ␥-radiation-induced mortality in mice and aided recovery from the radiation-induced loss of body weight after 8 Gy exposures.…”

Section: Introductionmentioning

confidence: 99%

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Radio-mitigation effect of poly-MVA after exposure to an acute dose of gamma radiation

El-Marakby

Selim

Desouky

et al. 2016

Journal of Taibah University for Science

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Adult male rats were exposed to a 6 Gy single dose from a Cs-137 source. The radio-mitigation effect of poly-MVA was evaluated by daily administration of 2 ml/kg of body weight immediately after irradiation for two weeks. The morphological changes in the red blood cells were studied. The osmotic fragility and rheological properties of blood, the alteration in the contents of antioxidant enzymes (glutathione, catalase and superoxide dismutase) and lipid peroxidation in hepatic cells were determined. The results showed that exposure to radiation resulted in significant changes in cellular antioxidant enzymes (GSH, catalase and SOD) and a decrease in the blood Bingham viscosity, yield stress and aggregation index. Furthermore, it induced a slight increase in the average osmotic fragility of red blood cells accompanied by a decrease in osmotic dispersion, as well as a modification of red blood cell morphology. It also caused a significant increase (75%) in the lipid peroxidation 1 day after exposure to radiation, which persisted until the 14 th day recorded after irradiation. Oral administration of poly-MVA after irradiation reduced the radiation-induced damage, as seen in the non-significant change in lipid peroxidation compared to the control. It also resulted in improvement in the observed parameters.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radio-mitigation effect of poly-MVA after exposure to an acute dose of gamma radiation

El-Marakby

Selim

Desouky

et al. 2016

Journal of Taibah University for Science

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“…The antioxidant status in the liver, kidney and brain of mice after exposure to 2-6 Gy radiation were also measured recently [Menon et. al., 2009].…”

Section: Catalytic Electronic and Therapeutic Properties Of Palladimentioning

confidence: 99%

Free Radicals in Neurodegenerative Diseases: Modulation by Palladium α-Lipoic Acid Complex

Krishnan¹,

Garnett²,

Antonawich³

2011

Neurodegenerative Diseases - Processes, Prevention, Protection and Monitoring

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Our research is based on the need for modern medicine to develop a safe and nontoxic product with a wide spectrum of uses. We strongly believe that one of the best ways to achieve this is to have a product that participates actively in most of the roles played by the mitochondria for optimal cellular function. Mitochondria are ubiquitous, and taking care of mitochondria is similar to taking care of all the parts leading to greater achievements than the sum of the parts [Krishnan et. al., 2011]. Oxidative stress is caused by the chemical imbalance between reactive oxygen species (ROS) production and their breakdown by antioxidants. Overabundance of ROS has been found during neuronal development, as well as in numerous neuropathological conditions. A predominant feature of neuronal injury is the onset of oxidative stress. Oxidative stress and mitochondrial dysfunction have been closely associated in many subcellular, cellular, animal, and human studies of both acute brain injury such as ischemia and stroke and neurodegenerative processes such as Parkinson's, Alzheimer's and Huntington's. While the oxidative stress occurs chronically in Alzheimer's disease, it is more acute in ischemic reperfusion injury. The consequences of mitochondrial dysfunction include DNA and protein damage, lipid peroxidation, disruption of the mitochondrial permeability transition, Ca 2+ homeostasis, and triggering apoptosis. It is essential to have a healthy mitochondria contributing substantially to the physical, mental, and emotional elements needed to support the well being of patients suffering from brain injury or neurodegenerative diseases. Energy metabolism, calcium regulation, and apoptosis-signaling pathways are the major roles of mitochondria. Energy requirements dictate the number of mitochondria in a cell [Beattie, 2002; Nagley et. al., 2010]. Cardiac and skeletal muscles, the brain, and the liver have the most mitochondria because of their high metabolic activities. These cells are also exposed to the most oxidative stress because the source of free radical production is also the mitochondria. Due to low levels of antioxidants in neurons, they are intrinsically illequipped to defend against an increase in oxidative stress. Glial cells including astrocytes play a supplementary role in antioxidant defense of neurons [Higgins et. al., 2010]. Our search for an extremely safe (up to 40 mL/day, 0.037 M aqueous solution) and nontoxic therapeutic agent resulted in the development of a novel redox molecule, "Palladium α-Lipoic Acid Complex" that is active in mitochondrial cellular metabolism and other www.intechopen.com Neurodegenerative Diseases-Processes, Prevention, Protection and Monitoring 90 functions. The selection of the naturally occurring coenzyme,-lipoic acid, as our ligand was based on its safety as well as its redox, antioxidant, and fatty acid properties. After selecting the ligand that plays a critical role in biological energy metabolism and numerous other functions, we wanted to tweak the properties of the ligand by co...

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“…1 Administration of POLY-MVA has been shown to alleviate radiation-induced lowering of tissue antioxidant levels in whole-body irradiated mice. 2 In the present study, POLY-MVA was investigated for its radioprotective effects in terms of spleen colony formation, prevention of cellular DNA damage, and animal survival to expand the scope of its use as a better radioprotective agent.…”

Section: Introductionmentioning

confidence: 99%

Radioprotection by α-Lipoic Acid Palladium Complex Formulation (POLY-MVA) in Mice

Ramachandran

Krishnan²,

Nair³

2010

Cancer Biotherapy and Radiopharmaceuticals

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The dietary supplement, POLY-MVA, containing palladium alpha-lipoic acid complex was examined for its efficacy as a radioprotector in mice exposed to whole-body gamma-radiation. Oral administration of POLY-MVA enhanced endogenous spleen colony formation in animals exposed to a sublethal dose of 6 Gy gamma-radiation. Alkaline comet assay revealed that the nuclear DNA comet parameters such as percent DNA in tail, tail length, tail moment, and olive tail moment, of the bone marrow cells and spleen cells, were found increased following whole-body gamma-irradiation. The radiation-induced DNA damage in these cells was reduced when POLY-MVA was administered to animals exposed to a lethal dose of 8 Gy whole-body gamma-radiation. The administration of POLY-MVA significantly reduced the gamma-radiation-induced mortality and also aided recovery from the radiation-induced loss of body weight in mice surviving after 8 Gy gamma-radiation exposure. These results suggest the potential use of POLY-MVA as a radioprotector in cases of planned radiation exposures.

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Protection from gamma-radiation insult to antioxidant defence and cellular DNA by POLY-MVA, a dietary supplement containing palladium-lipoic acid formulation

Cited by 9 publications

References 0 publications

Radio-mitigation effect of poly-MVA after exposure to an acute dose of gamma radiation

Radio-mitigation effect of poly-MVA after exposure to an acute dose of gamma radiation

Free Radicals in Neurodegenerative Diseases: Modulation by Palladium α-Lipoic Acid Complex

Radioprotection by α-Lipoic Acid Palladium Complex Formulation (POLY-MVA) in Mice

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