Protection of burn-induced skin injuries by the flavonoid kaempferol

Kim, Dongbum; Lee, Soo Hyoung; Seo, Jae Nam; Rhee, Jae Won; Park, Jae Bong; Kim, Yong Sun; Choi, Ihn Geun; Kim, Young Eun; Lee, Young Hee; Kwon, Hee Jung

doi:10.5483/bmbrep.2010.43.1.046

Cited by 28 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several reports have shown that thermal burn injury induces inflammatory cell infiltration in the dermis as well as thickening of the epidermis (1)(2)(3)(4)(5)38). Here, we report that infiltration of inflammatory cells in the dermis as well as thickening of the epidermis are increased by LPS or CpG-DNA treatment at thermal injury-induced burned areas of skin in mice (Fig.…”

Section: Effects Of Lps and Cpg-dna On Thermal Injury-induced T Cell supporting

confidence: 54%

“…Previously, we showed that thermal injury-induced macroscopic appearances of lesions are spontaneously healed after 21 days via regeneration and repair (scar formation) (38). Therefore, we examined the effect of LPS and CpG-DNA on the injury-induced macroscopic appearances of lesions over 14 days.…”

Section: Immunostimulatory Effects Of Lps and Cpg-dna Upon Thermal Inmentioning

confidence: 99%

“…Mice were anesthetized with Zoletil 50+Rompun, and all efforts were made to minimize suffering. Induction of thermal injury in the back skin was performed as reported previously (38). In brief, hair on the skin from the back of the animals (back skin) was shaved off with electronic clippers 3 days before each experiment.…”

Section: Animals and Induction Of Thermal Injurymentioning

confidence: 99%

See 2 more Smart Citations

Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

Kim

Cho²,

Seo³

et al. 2011

BMB Reports

Self Cite

View full text Add to dashboard Cite

Section: Effects Of Lps and Cpg-dna On Thermal Injury-induced T Cell supporting

confidence: 54%

Section: Immunostimulatory Effects Of Lps and Cpg-dna Upon Thermal Inmentioning

confidence: 99%

Section: Animals and Induction Of Thermal Injurymentioning

confidence: 99%

See 1 more Smart Citation

Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

Kim

Cho²,

Seo³

et al. 2011

BMB Reports

Self Cite

View full text Add to dashboard Cite

“…Although there are insufficient data to provide conclusive evidence on the health effects of most flavonoids (Kay 2010), the in vivo effects of kaempferol have been shown in several animal models, such as allergic lung disease (Medeiros et al 2009), carrageenan induced rat air pouch model (Mahat et al 2010), thermal burn-induced skin injuries (Park et al 2010), and mouse model of osteosarcoma (Huang et al 2010b). These reports imply that kaempferol may have clinical application, especially the safety issue of kaempferol has been discussed (Lee et al 2009;Li et al 2008).…”

Section: Discussionmentioning

confidence: 98%

Kaempferol from Semen cuscutae attenuates the immune function of dendritic cells

Lin

Chen

et al. 2011

Immunobiology

View full text Add to dashboard Cite

“…Moreover, under daily treatment with 100 µL kaempferol in ethanol 100 µM for 10-30 d, the inflammatory cell infiltrates in the dermis and thickening of the epidermis in the burned area of skin were clearly ameliorated. 11) These results demonstrated the efficacy of kaempferol in thermal burn-induced skin injuries. Oral administration of kaempferol results in very low bioavailability (2%) because it exhibits an extensive first-pass metabolism.…”

mentioning

confidence: 57%

The Effect of Submicron Emulsion Systems on Transdermal Delivery of Kaempferol

Chao

Huang

et al. 2012

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

In this study, submicron emulsions have been employed as a carrier for the topical application of kaempferol. The effect of components of submicron emulsions on the physicochemical properties and permeation capability of drug were evaluated. In case of drug-loaded submicron emulsions, the cumulative amount over 12 h (Q 12 h ), lag time and deposition in skin amount ranged from 13.0 3.4 to 236.1 21.2 µg/cm 2 , 1.7 to 5.3 h, and 1.10 to 7.76 µg/cm 2 , respectively, which indicated that the permeation parameters of kaempferol were markedly influenced by the component ratio. Kaempferol dispensed in isopropyl myristate was used as the control. The Q 12 h , lag time and deposition amount in skin were 4.2 1.8 µg/cm 2 , 6.0 h and 2.25 0.60 µg/cm 2 , respectively. The data showed that used appropriate submicron emulsions as vehicle could significantly increase the Q 12 h and deposition amount in skin and shorten the lag time, demonstrating that submicron emulsions have a potent enhancement effect for kaempferol transdermal delivery.Key words kaempferol; submicron emulsion; in vitro permeation study; permeation parameter; skin deposition amount Kaempferol (C 15 H 10 O 6 , molecular weight 286.24, mp 276°C) is one of the phytoestrogens, and it is found in berries and Brassica and Allium species. It has been recognized to have antioxidative, anti-inflammatory, antiallergic, and anticancer properties.1-10) Regarding its anti-inflammatory properties, Wang et al. 1) reported that kaempferol dose-dependently inhibited inducible nitric oxide synthase (iNOS) mRNA expression and prostaglandin E2 production, in part through a reducing nuclear factor-kappaB (NF-kappaB) signaling pathway. Park et al. 4) reported that kaempferol could inhibit the activation of inflammatory NF-kappaB transcription factor through nuclear factor-inducing kinase (NIK)/IkappaB kinase (IKK) and mitogen-activated protein kinases (MAPKs) in aged rat kidney. Moreover, under daily treatment with 100 µL kaempferol in ethanol 100 µM for 10-30 d, the inflammatory cell infiltrates in the dermis and thickening of the epidermis in the burned area of skin were clearly ameliorated.11) These results demonstrated the efficacy of kaempferol in thermal burn-induced skin injuries. Oral administration of kaempferol results in very low bioavailability (2%) because it exhibits an extensive first-pass metabolism.12) Hence, kaempferol is a good candidate for topical application.Submicron emulsion systems are fine oil-in-water or waterin-oil dispersions with small droplet sizes in the range of 100-600 nm. 13) They offer many advantages over other traditional vehicles including ease of manufacturing, thermodynamic stability, enhanced drug solubilization and increased drug permeation rate.14) Submicron emulsions are typically composed of large amounts of surfactants and oil, which are irritants for skin. However, previous studies [14][15][16][17] reported that submicron emulsion did not cause barrier perturbation of the skin, and that they can even decrease the skin irritation caus...

show abstract

Protection of burn-induced skin injuries by the flavonoid kaempferol

Abstract: Thermal burn injury induces inflammatory cell infiltrates in

Cited by 28 publications

References 28 publications

Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

Kaempferol from Semen cuscutae attenuates the immune function of dendritic cells

The Effect of Submicron Emulsion Systems on Transdermal Delivery of Kaempferol

Contact Info

Product

Resources

About