Protection of CCl<sub>4</sub>-induced hepatic and renal damage by linalool

Mazani, Mohammad; Rezagholizadeh, Lotfollah; Shamsi, Saeedeh; Mahdavifard, Sina; Ojarudi, Masoud; Salimnejad, Ramin; Salimi, Ahmad

doi:10.1080/01480545.2020.1792487

Cited by 22 publications

(16 citation statements)

References 42 publications

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“…Toxic effects on various organs of the human body, especially the liver, have limited its administration [8,10]. Accumulating evidence demonstrates that CP-induced ROS production causes peroxidative damage and severe hepatotoxic effects [22,23]. Most drugs are not toxic to the liver, but their metabolites may be toxic to the liver, known as metabolic bioactivation [27].…”

Section: Discussionmentioning

confidence: 99%

“…PAS staining was used to evaluate glycogen content in (glycogen storage) in hepatocytes. Finally, to evaluate the tissue changes in each sample, 12 images were randomly selected then the histological index was calculated using semiquantitative method described by Frei et al The level of damage was recorded based on (0-3) grades in which grade 0 = no damage, 1 = mild damage, 2 = moderatedamage, and 3 = severe damage (20)(21)(22)(23).Slides were viewed and photographed using a camera microscope at 400x magnification in at least three random microscopic fields from each animal by two expert pathologists without knowledge of the treatment groups [22][23][24][25].…”

Section: Evaluation Of Histopathological Alterationsmentioning

confidence: 99%

“…The supernatants were utilized for the measurement of MDA level and TAC. The MDA reacts with Thiobarbituric acid (TBA) and resulted in a red complex [22]. To measure the total antioxidant capacity Randox kit (UK) was used.…”

Section: Determination Of Liver Malondialdehyde and Total Antioxidant Capacitymentioning

confidence: 99%

“…To measure the total antioxidant capacity Randox kit (UK) was used. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS + ) cation generation followed by its inhibition by the tissue antioxidant compounds is the base of total antioxidant capacity evaluation [22]. Also, the Bradford assay was used to measure the concentration of proteins in the tissue sample [26].…”

Section: Determination Of Liver Malondialdehyde and Total Antioxidant Capacitymentioning

confidence: 99%

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Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

et al. 2021

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Background Despite its vast spectrum of clinical usage, cyclophosphamide (CP) exerts many adverse impacts, including hepatotoxicity. Antioxidant properties of ghrelin might protect the liver from CP-induced toxicity. The current study aimed to assess the protective impacts of ghrelin on CP-induced liver toxicity. Methods Forty male mice were randomly divided into four groups (n=10) Group 1 as control received no intervention,group 2 received cyclophosphamide (CP) (100 mg/kg, i.p.) for five weeks and once a week. Group 3 received CP+ghrelin (CP+G), (80 µg/kg daily, i.p.) for five weeks. Group 4 received ghrelin with above-mentioned dose. At the end of the experiment, the mice were sacrificed to remove liver tissuesfor histological and biochemical examination. Results Malondialdehyde (MDA) level increased after CP treatment but ghrelin administration significantly decreased the level of MDA (P<0.05). Measurement of the total antioxidant capacity (TAC) noted a significant decrease in the CP group against the control group (P<0.05). Ghrelin treatment in the CP+G group considerably increased the TAC activity when compared to the CP group (P<0.05). Histological examinations also confirmed the hepatocyte necrosis, local bleeding and inflammation, vacuolation, and sinusoidal dilation in the CP group, ghrelin administration reduced the destructive effects of CP on the liver significantly (P<0.05). Conclusion Our results reveal the hepatoprotective effect of ghrelin against CP. Therefore, ghrelin might be useful in protecting the body against the adverse impacts of injuries induced by chemotherapeutic drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Histopathological Alterationsmentioning

confidence: 99%

Section: Determination Of Liver Malondialdehyde and Total Antioxidant Capacitymentioning

confidence: 99%

Section: Determination Of Liver Malondialdehyde and Total Antioxidant Capacitymentioning

confidence: 99%

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Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

et al. 2021

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“…Nowadays, there is a market for these compounds that are requested by patients; additionally, they are produced chemically by pharmaceutical companies. Mostly the herbal drugs can have improvement effects on the diverse diseases and toxicities [5]. Linalool (LIN), one of these herbal drugs and an important component of the essential oil identified as a volatile component from many natural aromatic plants, has numerus pharmacological effects, including antitumor, anti-inflammatory, antioxidant and antimicrobial properties [6].…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Evaluation of Linalool Effect against Renal Ischemia- Reperfusion Injury in Rats

2021

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Background Kidney ischemia reperfusion (IR) is an important cause of renal dysfunction. The hypoxic conditions in ischemic damage result in the formation of free radicals and apoptotic death of renal cells. We evaluated the renoprotective effects of linalool in IR- induced renal injury. Methods Wistar rats were divided into three groups of six rats; namely, control group, IR group, and linalool + IR group. The animals were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. Linalool (40mg/kg) was administered before ischemia. After 24h reperfusion, the kidney tissues were obtained for detection of miR-21, HSP 70 and caspase-3 expression levels and histological studies. Also, the blood samples were collected for the measurement of biochemical parameters. Results IR significantly increased the expression of miR-21, HSP70 and capase-3 and the serum levels of BUN-Cr, ALT, AST and ALP enzymes. Furthermore, histological findings of the IR group confirmed that there were acute tubular necrosis and lymphocyte infiltration in the renal tissues. Treatment with linalool improved the renal function and morphological changes. Conclusion It seems that linalool could exert a nephroprotective effect via a number of mechanisms in renal IR injury.

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Zinc citrate‐coated whey protein nanoparticles alleviate kidney damage and the disturbances in inflammatory gene expression in rats

El‐Nekeety

Hassan

Abdel-Aziem

et al. 2023

J Biochem & Molecular Tox

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This work was conducted to synthesize whey protein nanoparticles (WPNPs) for the coating of zinc citrate (Zn CITR) at three levels and to study their protective role against CCl4‐induced kidney damage and inflammatory gene expression disorder in rats. Seventy male Sprague‐Dawley rats were divided into seven groups and treated orally for 4 weeks as follows; the control group, the group treated twice a week with CCl4 (5 mL/kg b.w), the groups received CCl4 plus WPNPs (300 mg/kg b.w); the group received 50 mg/kg b.w of Zn CITR or the three formulas of Zn CITR‐WPNPs at low, medium and high doses (LD, MD, and HD). Blood and kidney samples were collected for different assays and histological analyses. The fabricated particles were semispherical, with an average size of 160 ± 2.7, 180 ± 3.1, and 200 ± 2.6 nm and ζ potential of −126, −93, and −84 mV for ZN CITR‐WPNPs (LD), Zn CITR‐WPNPs (MD), and ZN CITR‐WPNPs (HD), respectively. CCl4 significantly increased (p ≤ 0.05) kidney function indices, oxidative stress markers, messenger RNA expression of transforming growth factor‐β1, interleukin (IL)‐1β, IL‐10, IL‐6, inducible nitric oxide synthase, and tumor necrosis factor‐α and significantly decreased (p ≤ 0.05) renal superoxide dismutase, catalase, and glutathione peroxidase along with the histological changes in the kidney tissues. WPNPs, Zn CITR, and Zn CITR loaded WPNPS showed a protective effect against these complications and Zn CITR‐WPNPs (LD) was more effective. WPNPs can be used effectively for coating Zn CITR at a level of 7 mg/g WPNPs to be used as a supplement for the protection of the kidney against different toxicants to enhance immunity and avoid harm of excess Zn.

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Protection of CCl₄-induced hepatic and renal damage by linalool

Cited by 22 publications

References 42 publications

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Mechanistic Evaluation of Linalool Effect against Renal Ischemia- Reperfusion Injury in Rats

Zinc citrate‐coated whey protein nanoparticles alleviate kidney damage and the disturbances in inflammatory gene expression in rats

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Protection of CCl4-induced hepatic and renal damage by linalool

Cited by 22 publications

References 42 publications

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Mechanistic Evaluation of Linalool Effect against Renal Ischemia- Reperfusion Injury in Rats

Zinc citrate‐coated whey protein nanoparticles alleviate kidney damage and the disturbances in inflammatory gene expression in rats

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Protection of CCl₄-induced hepatic and renal damage by linalool