Protection of coenzyme Q10 against contrast-induced acute kidney injury in male diabetic rats

Abstract

Background Diabetes mellitus (DM) is a major risk factor for contrast-induced acute kidney injury (CI-AKI). DM and CI-AKI result in oxidative damage and inflammation that can be reduced when treated with the coenzyme Q-10 (CoQ10). The aim of this study was to investigate the therapeutic potential of CoQ10 in renal function, renal hemodynamics, oxidative profile and renal histology in diabetic rats subjected to CI-AKI. Methods Wistar rats, male, randomized into five groups: citrate: control animals received citrate buffer (streptozotocin vehicle, 0.4 mL); Tween: control animals of CoQ10 treatment received 1% Tween 80 (CoQ10 vehicle, 0.5 mL); DM: animals that received streptozotocin (60 mg/kg); DM + IC: DM animals treated with iodinated contrast (IC, 6 mL/kg); DM + IC + CoQ10: DM animals treated with CoQ10 (10 mg/kg) and that received IC (6 mL/kg). The protocols lasted 4 weeks. An evaluation was made to measure renal function, inulin clearance and serum creatinine, renal hemodynamics by renal blood flow (RBF) and renal vascular resistance (RVR), markers of oxidative stress such as urinary peroxides and nitrate, lipid peroxidation, thiols in renal tissue and renal histological analysis. Results DM animals showed reduced renal function, which was followed by an increase inserum creatinine and significant reduction of inulin clearance and RBF. It was noticed an increase in RVR and redox imbalance with higher urinary peroxides and nitrate lipid peroxidation levels with depletion of thiols in renal tissue. IC treatment exacerbated these changes in DM + IC. CoQ10 administration ameliorated renal function, prevented hemodynamic changes and neutralized oxidative damage and progression of the histologic damage in the DM + IC + CoQ10 group. Conclusion This study demonstrated the renoprotection properties of CoQ10 in an experimental model of risk factor of DM for CI-AKI. CoQ10 presented an antioxidant effect on the CI-AKI in male diabetic rats by improving renal function and renal hemodynamics, preserving morphology and reducing oxidative stress.

The Effects of Coenzyme Q10 on Contrast-Induced Acute Kidney Injury in Type 2 Diabetes: A Randomized Clinical Trial

“… 39 40 41 No substantial direct damage to the kidneys occur in the early stages of diabetes, however, oxidative stress hypoxia-inducible factors are key to the changes in the renal blood flow during diabetes. 42 43 …”

Association between fasting stress hyperglycemia ratio and contrast-induced acute kidney injury in coronary angiography patients: a cross-sectional study

“…The association between high level fasting SHR and CI-AKI can be elucidated through several mechanisms. Firstly, stress hyperglycemia may engender increased stress responses, encompassing mitochondrial dysfunction, oxidative stress, endoplasmic reticulum stress, thereby collectively precipitating a cascade of pathological alterations within renal tissue, ultimately culminating in renal dysfunction and an escalated susceptibility to CI-AKI ( 34 – 39 ). Secondly, the equilibrium between nitric oxide (NO) bioavailability and the accumulation of reactive oxygen species (ROS) in endothelial cells may be disrupted by stress hyperglycemia, which may cause endothelial dysfunction and subsequently induce CI-AKI ( 40 – 42 ).…”

Ubiquinol attenuates γ-radiation induced coronary and aortic changes via PDGF/p38 MAPK/ICAM-1 related pathway

“…Each capsule was evacuated and dissolved in 1% tween 80 solution and administrated to rats at a dose of 10 mg/kg/day, p.o. according to Couto, et al 57 .…”