Protection of deoxyribose and DNA from degradation by using aqueous extracts of several wild plants

Gião, Maria S.; González-SanJosé, María L.; Rivero-Pérez, María D.; Kosinska, Monika; Pintado, Manuela; Malcata, F. Xavier

doi:10.1002/jsfa.3128

Cited by 22 publications

(26 citation statements)

References 24 publications

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“…In fact, the extracts of several medicinal plants have been reported to exhibit an antioxidant capacity Gião, Pereira, Fonseca, Pintado, & Malcata, 2009) as well as a protective effect against the degradation of deoxyribose and DNA (Gião et al, 2008) and hepatic oxidative stress (Gião et al, 2010). Likewise, an antioxidant activity and a protective effect against DNA oxidative damage have been observed for sugar molasses (Guimarães et al, 2007).…”

Section: Introductionmentioning

confidence: 95%

Antimicrobial and antioxidant activities of Chinese sumac (Rhus typhina L.) fruit extract

2011

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Section: Introductionmentioning

confidence: 95%

Antimicrobial and antioxidant activities of Chinese sumac (Rhus typhina L.) fruit extract

2011

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“…Variations in the environmental conditions away from those which are potential result in the accumulation of potentially harmful reactive oxygen species (ROS), viz., superoxide (O 2 •-) and hydroxyl (OH • ) radical, which can overcome the antioxidant defenses of the host, thus producing (often irreversible) cellular damage (Grace and Logan 1996;Giao et al 2008). ROS also include singlet oxygen ( 1 O 2 ), superoxide radical anions and hydrogen peroxide (H 2 O 2 ) (Asada 1999).…”

Section: Introductionmentioning

confidence: 99%

Effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea

Khalil

Asker

El-Sayed

et al. 2009

World J Microbiol Biotechnol

148

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The goal of the present investigation was to study the effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea when exposed to different pH values. The two tested microalgae could grow in a wide range of pH (4-9 for D. bardawil and 4-10 for C. ellipsoidea). The dry weight gain and the biochemical components of D. bardawil were greatly enhanced at pH 7.5. In contrast, dry weight and carbohydrate content of C. ellipsoidea attained their maximum values at the alkaline pH. On the other hand, the protein content of C. ellipsoidea recorded its highest value at pH 4, while the pigment content of the same alga was highest at pH 4, 6, and 7.5 and decreased at alkaline pH. Both pH 6 and pH 9 stimulated the accumulation of β-carotene, vitamin E and vitamin C in D. bardawil, with the highest values of the three compounds recorded at pH 9. In the case of C. ellipsoidea, β-carotene content increased at pH 6 and pH 10 as compared with the control, but the amount of β-carotene was much higher at pH 6 than at pH 10. Vitamin E content was higher in C. ellipsoidea cells at pH 10 than at pH 6. Both pH 6 and pH 10 caused a significant decline in vitamin C content of C. ellipsoidea.

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“…22 On the other hand, nanoparticles can be evaluated for their ability to act, under specific conditions, as prooxidantfree radicals, thus leading to DNA oxidation, which is often a concentration dependent effect. 23 For such purpose, in vitro tests using DNA agarose gel can be done to obtain DNA strands in intact or fragmented (oxidized) state. In this regard, although most of the nanoparticles toxicity tests are done in red blood cells (RBCs) and macrophages, lymphocytes are a feasible good alternative.…”

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confidence: 99%

“…DNA interaction assay was performed according to the protocol previously described. 23 A 400 µL aliquot of sample was initially prepared and processed (as will be detailed), as well as 100 mM standard solution of ascorbic acid. This method includes assays of both controls and samples and evaluates both anti-and prooxidant properties.…”

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confidence: 99%

Safety profile of solid lipid nanoparticles loaded with rosmarinic acid for oral use: in vitro and animal approaches

Madureira

Nunes

Fernandes

et al. 2016

IJN

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Rosmarinic acid (RA) possesses several protective bioactivities that have attracted increasing interest by nutraceutical/pharmaceutical industries. Considering the reduced bioavailability after oral use, effective (and safe) delivery systems are crucial to protect RA from gastrointestinal degradation. This study aims to characterize the safety profile of solid lipid nanoparticles produced with Witepsol and Carnauba waxes and loaded with RA, using in vitro and in vivo approaches, focused on genotoxicity and cytotoxicity assays, redox status markers, hematological and biochemical profile, liver and kidney function, gut bacterial microbiota, and fecal fatty acids composition. Free RA and sage extract, empty nanoparticles, or nanoparticles loaded with RA or sage extract (0.15 and 1.5 mg/mL) were evaluated for cell (lymphocytes) viability, necrosis and apoptosis, and antioxidant/prooxidant effects upon DNA. Wistar rats were orally treated for 14 days with vehicle (control) and with Witepsol or Carnauba nanoparticles loaded with RA at 1 and 10 mg/kg body weight/d. Blood, urine, feces, and several tissues were collected for analysis. Free and loaded RA, at 0.15 mg/mL, presented a safe profile, while genotoxic potential was found for the higher dose (1.5 mg/mL), mainly by necrosis. Our data suggest that both types of nanoparticles are safe when loaded with moderate concentrations of RA, without in vitro genotoxicity and cytotoxicity and with an in vivo safety profile in rats orally treated, thus opening new avenues for use in nutraceutical applications.

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Protection of deoxyribose and DNA from degradation by using aqueous extracts of several wild plants

Cited by 22 publications

References 24 publications

Antimicrobial and antioxidant activities of Chinese sumac (Rhus typhina L.) fruit extract

Antimicrobial and antioxidant activities of Chinese sumac (Rhus typhina L.) fruit extract

Effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea

Safety profile of solid lipid nanoparticles loaded with rosmarinic acid for oral use: in vitro and animal approaches

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