Protection of Intestinal Occludin Tight Junction Protein by Dietary Gangliosides in Lipopolysaccharide‐induced Acute Inflammation

Park, Eek J.; Thomson, A. D.; Clandinin, M. T.

doi:10.1097/mpg.0b013e3181ae2ba0

Cited by 73 publications

(52 citation statements)

References 51 publications

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“…An in depth knowledge functional properties of milk compounds is a prerequisite in the design of such foods. Further development of economically viable industrial processes in the production of bioactive milk components is critical challenge to the food technologists [21,23,24]. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.…”

Section: Discussionsupporting

confidence: 55%

“…The FABP shows immunostimulating, opioid and antioxidant peptides. Because of MFGM's phospholipid enrichment and unique biochemical nature, we hypothesized that diets which include MFGM as a fat source might offer better protection against an exaggerated inflammatory response [23,24,25]. Compared to MFGM very little literature is available on colostrum fat globule membranes (CFGM).…”

Section: Introductionmentioning

confidence: 99%

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Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

2017

J App Biol Biotech

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Colostrum is the first liquid food for the new born baby, secreted after the parturition. After few days of maturation the colostrum turns to milk. The cream or fat fraction of milk consists of fat droplets composed primarily of triacylglycerols that are secreted from the apical surface of the mammary cells surrounded by cellular thin membranes membrane, the fat globule membrane (FGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, antioxidant, bactericidal, suppressors of multiple sclerosis. FGM was isolated from buffalo colostrum and milk, the fat content was > 2-fold higher in the former than in the latter. The colostrum fat globule membrane (CFGM) fraction also showed significantly higher protein content than milk fat globule membrane (MFGM) extract. Further, the SDS-PAGE separation of the total proteins from the CFGM and MFGM showed differential banding pattern. Antioxidative property of the two FGM extracts was deliberated based on their free radical scavenging ability and reducing power. CFGM was found to exhibit a relatively higher antioxidant and reductive capacity than the MFGM fractions. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

2017

J App Biol Biotech

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“…LPS was shown to reduce ZO-1 expression in nontumorgenic epithelial monolayers [30], to degrade gut occludin in rats [31] and significantly disrupting barrier function. Decreased ZO-1 protein expression has been found to correlate with increased paracellular permeability in endotoxemic animals [25].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide Cathelicidin-BF prevents intestinal barrier dysfunction in a mouse model of endotoxemia

Song

Zong

Zhang

et al. 2015

International Immunopharmacology

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“…The intestinal mucosa is a physical and metabolic barrier against toxins and pathogens in the lumen. The barrier regulates macromolecule trafficking between the lumen and the internal milieu, and it protects the host by preventing harmful solutes, microorganisms, toxins, and luminal Ags from entering the body (58). Compromising the barrier function of the intestinal mucosa can result in an increased exposure of the host to luminal Ags and pathogens, leading to inflammation (59), and is a major contributing factor in multiple pathological conditions of the gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Porcine β-Defensin 2 Attenuates Inflammation and Mucosal Lesions in Dextran Sodium Sulfate–Induced Colitis

Han¹,

Zhang

Xia

et al. 2015

The Journal of Immunology

140

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Intestinal permeability plays a critical role in the etiopathogenesis of ulcerative colitis. Defensins, including porcine β-defensin (pBD)2, are crucial antimicrobial peptides for gut protection owing to their antibacterial and immunomodulatory activities. The purpose of this study was to investigate the protective effects of pBD2 on mucosal injury and the disruption of the epithelial barrier during the pathological process of dextran sodium sulfate (DSS)–induced colitis. The effects and mechanism of pBD2 were evaluated both using a DSS-induced C57BL/6 mouse model and, in vitro, using Caco-2 and RAW264.7 cells. DSS-induced colitis was characterized by higher disease activity index, shortened colon length, elevated activities of myeloperoxidase and eosinophil peroxidase, histologic evidence of inflammation, and increased expression levels of TNF-α, IL-6, and IL-8. pBD2 increased the expression of zonula occludens-1, zonula occludens-2, claudin-1, mucin-1, and mucin-2 mRNA and proteins, and it decreased permeability to FITC-D, as well as apoptosis, in DSS-treated mice. pBD2 also decreased inflammatory infiltrates of the colon epithelium. In Caco-2 cells, pBD2 increased transepithelial electrical resistance and mucin mRNA expression, and it decreased the permeability of FITC-D while preserving the structural integrity of the tight junctions. The effects of pBD2 appeared to be through upregulation of the expression of genes associated with tight junctions and mucins, and by suppressing DSS-induced increases in inflammation, inducible NO synthase, cyclooxygenase-2, and apoptosis. These results show that pBD2 improves DSS-induced changes in mucosal lesions and paracellular permeability, possibly by affecting the activation of NF-κB signaling. The present study demonstrates that intrarectal administration of pBD2 may be a novel preventive option for ulcerative colitis.

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Protection of Intestinal Occludin Tight Junction Protein by Dietary Gangliosides in Lipopolysaccharide‐induced Acute Inflammation

Abstract: Dietary GGs inhibit degradation of gut occludin tight junction protein during LPS-induced acute inflammation. Thus, dietary GGs have a role in protecting the integrity of the intestinal barrier during acute gut inflammation.

Cited by 73 publications

References 51 publications

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

Antimicrobial peptide Cathelicidin-BF prevents intestinal barrier dysfunction in a mouse model of endotoxemia

Porcine β-Defensin 2 Attenuates Inflammation and Mucosal Lesions in Dextran Sodium Sulfate–Induced Colitis

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