Protection of laying chickens against the Canadian DMV/1639 infectious bronchitis virus infection through priming with heterologous live vaccine and boosting with heterologous or homologous inactivated vaccine

Hassan, Mohamed S.H.; Ali, Ahmed; Mahmoud, Motamed Elsayed; Altakrouni, Danah; Najimudeen, Shahnas M.; Abdul-Careem, Mohamed Faizal

doi:10.1016/j.virusres.2023.199281

Cited by 2 publications

(1 citation statement)

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“…Live attenuated IBV vaccines are effective; however, they carry the risk of reversion to virulence and often involve co-infection with other avian viruses, such as the H9N2 avian influenza virus, resulting in more severe outbreaks [11]. Inactivated IBVs are safe but costly, and elevated levels of protection will be achieved by co-administration with live attenuated vaccines [12,13]. Therefore, there is a need to develop a new vaccine, which not only does not depend on chicken embryos but is also cost-effective and capable of inducing high-efficiency and longlasting immune protection [14].…”

Section: Introductionmentioning

confidence: 99%

Salmonella typhimurium Vaccine Candidate Delivering Infectious Bronchitis Virus S1 Protein to Induce Protection

Liu,

Li,

et al. 2024

Biomolecules

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Infectious bronchitis (IB) is a highly infectious viral disease of chickens which causes significant economic losses in the poultry industry worldwide. An effective vaccine against IB is urgently needed to provide both biosafety and high-efficiency immune protection. In this study, the S1 protein of the infectious bronchitis virus was delivered by a recombinant attenuated Salmonella typhimurium vector to form the vaccine candidate χ11246(pYA4545-S1). S. typhimurium χ11246 carried a sifA− mutation with regulated delayed systems, striking a balance between host safety and immunogenicity. Here, we demonstrated that S1 protein is highly expressed in HD11 cells. Immunization with χ11246(pYA4545-S1) induced the production of antibody and cytokine, leading to an effective immune response against IB. Oral immunization with χ11246(pYA4545-S1) provided 72%, 56%, and 56% protection in the lacrimal gland, trachea, and cloaca against infectious bronchitis virus infection, respectively. Furthermore, it significantly reduced histopathological lesions in chickens. Together, this study provides a new idea for the prevention of IB.

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