Protection of Litopenaeus vannamei against the white spot syndrome virus using recombinant Pm-fortilin expressed in Pichia pastoris

Sinthujaroen, Patuma; Tonganunt-Srithaworn, Moltira; Eurwilaichitr, Lily; Phongdara, Amornrat

doi:10.1016/j.aquaculture.2014.10.024

Cited by 4 publications

(3 citation statements)

References 61 publications

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“…The addition of V. alginolyticus (Sun et al., ) or functional additives to feeds, such as protein (Sinthujaroen, Tonganunt‐Srithaworn, Eurwilaichitr, & Phongdara, ) and carbohydrates (Chang et al., ), has been reported to improve the ability of shrimp to resist WSSV. However, most of these reports have been explained from the perspective of improving shrimp immunity.…”

Section: Discussionmentioning

confidence: 99%

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Shan

Liu

et al. 2018

Aquacult Nutr

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This study aimed to investigate the effects of adding crude extracts of the extracellular protein (Ex‐Pro) and intracellular polysaccharides (In‐Poly) of Vibrio alginolyticus to diets, at a dosage of 10 g/kg, on the growth, energy metabolism and WSSV (White Spot Syndrome Virus) resistance of Litopenaeus vannamei (initial weight, 0.88 ± 0.04 g/shrimp). Growth and survival rate were not significantly affected by the crude extracts (p > 0.05). Shrimp fed Ex‐Pro crude extracts showed higher succinate dehydrogenase (SDH) activity in the hepatopancreas and muscle but lower lactate dehydrogenase (LDH) activity in the muscle (p < 0.05). The activities of hexokinase (HK), pyruvate kinase (PK), LDH and SDH in the hepatopancreas and the activity of SDH in the muscle were significantly increased by feeding In‐Poly crude extracts (p < 0.05). In contrast, the content of fatty acid synthase (FAS) in the muscle was significantly reduced by the crude extracts (p < 0.05). The contents of glucose and triglyceride and the activity of the electron transport system in the hepatopancreas were significantly increased by the crude extracts (p < 0.05), and the WSSV resistance of the shrimp was increased. These results indicated that the Ex‐Pro and In‐Poly crude extracts of V. alginolyticus could affect energy metabolism, and there was a correlation between WSSV resistance and energy metabolism in L. vannamei.

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Section: Discussionmentioning

confidence: 99%

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Shan

Liu

et al. 2018

Aquacult Nutr

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“…Suri Tani Pemuka with average body weight of 6.66± 0.08 g. Shrimp was reared in aquarium with water volume of 30 l, temperature of 27-28 °C, salinity of 30 ppt for one week and fed with commercial feed. Shrimp was tested for WSSV infection status with nested PCR to ensure that the shrimp was free from WSSV infection (Sinthujaroen et al, 2015).…”

Section: Test Subjectmentioning

confidence: 99%

“…15 shrimp was used for each replicate and observed until day 5 post infection. Shrimp was tested for WSSV infection status with nested PCR after day 5 post infection (Sinthujaroen et al, 2015).…”

Section: Experimental Designmentioning

confidence: 99%

Evaluation of Recombinant Viral Inhibitor Protein for Whiteleg Shrimp Resistance Against White Spot Syndrome Virus

Abdurrahman,

Alimuddin,

Sudrajat

et al. 2023

JFS

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Viral inhibitor protein (VIP) contributes to shrimp resistance against virus infection. VIP can be used as a new strategy to control white spot syndrome virus (WSSV) infection in shrimp culture by inhibiting its action. This study aimed to evaluate the application of VIP on the whiteleg shrimp (Litopenaeus vannamei) to inhibit the WSSV infection. Shrimp at an average body weight of 6.66±0.08 g was injected by recombinant VIP with two different concentrations (1 μg/g and 10 μg/g) and challenged with WSSV. As a positive control was the shrimp not injected with recombinant VIP and challenged with WSSV, and as a negative control was the shrimp not injected with recombinant VIP and not challenged with WSSV. The results showed that the survival rate of shrimp that were injected with recombinant VIP was significantly increased after being challenged with WSSV (P<0.05). The survival and immune response of shrimp that were injected with recombinant VIP were higher than the positive controls (P<0.05). In this study, the application of recombinant VIP can significantly increase the shrimp resistance against WSSV up to 95.65% higher than the positive control.

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Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii

et al. 2020

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Protection of Litopenaeus vannamei against the white spot syndrome virus using recombinant Pm-fortilin expressed in Pichia pastoris

Cited by 4 publications

References 61 publications

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Evaluation of Recombinant Viral Inhibitor Protein for Whiteleg Shrimp Resistance Against White Spot Syndrome Virus

Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii

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