Protection of Mice against Brucella Abortus by Immunization with Polyclonal Anti-Idiotype Antibodies

Beauclair, Kim D.; Khansari, David N.

doi:10.1016/s0171-2985(11)80329-3

Cited by 13 publications

(6 citation statements)

References 17 publications

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“…Subunit vaccines have been used but are not highly effective in protecting cattle from subsequent infection and disease (8,41). Factors which usually influence the development of immunity against brucellosis in mice are related to the nature and dose of immunogen and the manner in which the immunizing epitopes are presented to the host's immune surveillance system (2,3,5,10,19,20,27,31,32). The overall immunity induced involves both cell-mediated and humoral antibody effects, which probably act together in preventing and eliminating infection.…”

mentioning

confidence: 99%

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Pugh

Tabatabai

1994

Infect Immun

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A study was conducted to determine whether the covalent chemical modification of BruceUla abortus 19 salt-extractable proteins (BCSP) and BCSP derivatives would modulate the immune responses in BALB/c mice. Salt-extractable proteins BCSP 0-70 and BCSP 70-100 were modified with acetoacetic anhydride, and recombinant proteins rBCSP20 (20 kDa), rBCSP31 (31 kDa), and rBCSP45 (45 kDa) were modified with succinic and dodecanoyl anhydrides. Four weeks after mice were vaccinated with the different preparations, principal and control mice were challenge exposed with a virulent culture of B. abortus 2308, and mice were necropsied 2 weeks later. Serum samples were obtained immediately before mice were challenge exposed and at necropsy. Sera were tested for specific immunoglobulin M (IgM) and G (IgG) antibodies by using an enzyme-linked immunosorbent assay. Acylation decreased the immune responses (increased IgG antibodies and reduced spleen CFU and splenomegaly) induced by both BCSP 0-70 and BCSP 70-100. Modification of the recombinant proteins by dodecanoyl and succinic anhydrides had no effect on the protection induced; however, the IgG serologic responses to the homologous and heterologous proteins were altered. Monophosphoryl lipid A markedly enhanced the immunogenicity of BCSP 0-70.

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mentioning

confidence: 99%

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Pugh

Tabatabai

1994

Infect Immun

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“…Heat-inactivated antiserum was subjected to sequential affinity column chromatography on Sepharose CL-4B (Pharmacia LKB), covalently cross-linked to purified normal mouse IgG and Abl with coupling buffer (0.1 mol/L NaHCO3; 0.5 mol/L NaC1, pH 8.5) (Beauclair and Khausari, 1990). The unbound fraction from the first column was washed off using PBS, concentrated, dialysed, and passed through a second immunoaffinity column coupled to Abl.…”

Section: Purification Of the Rabbit Anti-idiotypic Iss/32 Antibodiesmentioning

confidence: 99%

Production and characterization of rabbit anti-idiotypic antibodies directed against a murine monoclonal anti-B. abortus antibody

et al. 1995

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The protective anti-beta abortus monoclonal antibody ISS/32 (Ab1) was used as an immunogen to induce anti-idiotypic antibodies (Ab2) in rabbits. The purpose was to produce and characterize anti-idiotypic antibodies that share conformational similarity with the corresponding bacterial epitope recognized by Ab1. The rabbit anti-IdAb so induced was isolated and affinity-purified. Its specificity for the paratope of Ab1 was determined by evaluating its ability to compete with B. abortus for binding to Ab1 in a competitive ELISA assay. The anti-idiotypic ISS/32 antibodies were able to compete with B. abortus for binding to Ab1 in a dose-dependent manner. Hence, the data indicated that the rabbit anti-Id ISS/32 antibodies reacted with or near the antigen-binding site of Ab1.

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“…Although several previous studies have demonstrated that immunization with anti-Id could induce protective immunity or antibody response to the pathogen, they required quantities of immunogens and repeated booster immunizations to induce sufficient immunity [1,2,7,13,17]. To examine the efficacy of the anti-Id on inducing protective immunity in this experiment, minimum booster immunizations were performed on the supposition that anti-Id would be practically used as immunogens against PrV.…”

Section: Protection Of Mice From Prv Infection By Immunization With Amentioning

confidence: 99%

Induction of protective immunity and neutralizing antibodies to pseudorabies virus by immunization of anti-idiotypic antibodies

Tsuda

Onodera

Sugimura

et al. 1992

Archives of Virology

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Xenogenic anti-idiotypic antibodies (anti-Id) were prepared in rabbits against three murine neutralizing monoclonal antibodies (MAbs) directed to pseudorabies virus glycoproteins. These anti-Id were highly specific to idiotopes on the corresponding MAb molecules. Because the binding of MAb to the corresponding anti-Id was inhibited by the addition of viral envelope protein, these anti-Id seemed to contain a subpopulation of antibodies against the antigen-combining site (paratope) or the region related to the paratope of the MAb molecules. One of the anti-Id to a MAb directed against glycoprotein gp50 induced neutralizing antibodies to PrV. Mice immunized with the anti-Id were protected from lethal infection of PrV.

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Protection of Mice against Brucella Abortus by Immunization with Polyclonal Anti-Idiotype Antibodies

Cited by 13 publications

References 17 publications

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Production and characterization of rabbit anti-idiotypic antibodies directed against a murine monoclonal anti-B. abortus antibody

Induction of protective immunity and neutralizing antibodies to pseudorabies virus by immunization of anti-idiotypic antibodies

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