Protection of mice against P. aeruginosa infections by large-scale affinity-purified human IgG specific to P. aeruginosa outer membrane proteins

Lee, Na-Gyong; Jung, Sang Bo; Ahn, Bo Young; Kim, Young Gi; Lee, Younha; Jeon, Yeong-Joong; Park, Wan Je

doi:10.1016/s0264-410x(99)00273-x

“…Anti‐ P. aeruginosa OMP IgG was prepared from a pooled normal human plasma (Blood Center of Korea Red Cross, Korea) using a large‐scale affinity column as described previously [15]. Briefly, an affinity column with a bed volume of 500 ml was prepared by covalent coupling of Sepharose 4B with the OMPs isolated from the four P. aeruginosa strains.…”

Section: Methodsmentioning

confidence: 99%

Conformation-dependent antibody response toPseudomonas aeruginosaouter membrane proteins induced by immunization in humans

Lee

¹

,

Ahn

²

,

Jung

³

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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Outer membrane proteins (OMPs) of pathogenic bacteria have been used as protective antigens in developing bacterial vaccines. In the present study, we compared the antibody responses to a Pseudomonas aeruginosa OMP vaccine elicited in humans and rabbits by immunization. Immunization with the vaccine induced high titers of serum IgG antibody both in rabbits and humans but reactivities of the induced antibodies with the OMPs were different. The rabbit immune sera recognized most of the OMPs in the vaccine both in immunoblot and immunoprecipitation analyses. In contrast, a great variation in band pattern and intensity was observed among the human immune sera in immunoblot analysis, but not in immunoprecipitation analysis. Denaturation of the OMPs did not affect the binding activity of the rabbit immune sera as determined by ELISA, but substantially reduced those of the human immune sera and anti-OMP IgG purified from a pooled normal human plasma. These data suggest that antibody response to P. aeruginosa OMPs elicited by immunization in humans is mainly directed against discontinuous or conformation-dependent epitopes, which should be taken into account in developing vaccines, especially for OMP-derived synthetic peptides. ß

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“…Anti-P. aeruginosa OMP IgG was prepared from a pooled normal human plasma (Blood Center of Korea Red Cross, Korea) using a large-scale a¤nity column as described previously [15]. Brie£y, an a¤nity column with a bed volume of 500 ml was prepared by covalent coupling of Sepharose 4B with the OMPs isolated from the four P. aeruginosa strains.…”

Section: Preparation Of Human Serum Iggmentioning

confidence: 99%

Conformation-dependent antibody response to Pseudomonas aeruginosa outer membrane proteins induced by immunization in humans

Lee¹

2000

FEMS Immunology and Medical Microbiology

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Outer membrane proteins (OMPs) of pathogenic bacteria have been used as protective antigens in developing bacterial vaccines. In the present study, we compared the antibody responses to a Pseudomonas aeruginosa OMP vaccine elicited in humans and rabbits by immunization. Immunization with the vaccine induced high titers of serum IgG antibody both in rabbits and humans but reactivities of the induced antibodies with the OMPs were different. The rabbit immune sera recognized most of the OMPs in the vaccine both in immunoblot and immunoprecipitation analyses. In contrast, a great variation in band pattern and intensity was observed among the human immune sera in immunoblot analysis, but not in immunoprecipitation analysis. Denaturation of the OMPs did not affect the binding activity of the rabbit immune sera as determined by ELISA, but substantially reduced those of the human immune sera and anti-OMP IgG purified from a pooled normal human plasma. These data suggest that antibody response to P. aeruginosa OMPs elicited by immunization in humans is mainly directed against discontinuous or conformation-dependent epitopes, which should be taken into account in developing vaccines, especially for OMP-derived synthetic peptides.

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New Perspectives on Prevention and Management of Pseudomonas Aeruginosa Infections

Rumbaugh

¹

,

Sawa

²

,

Wiener-Kronish

³

2003

Severe Infections Caused by Pseudomonas Aeruginosa

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Protection of mice against P. aeruginosa infections by large-scale affinity-purified human IgG specific to P. aeruginosa outer membrane proteins

Cited by 5 publications

References 36 publications

Conformation-dependent antibody response toPseudomonas aeruginosaouter membrane proteins induced by immunization in humans

Conformation-dependent antibody response toPseudomonas aeruginosaouter membrane proteins induced by immunization in humans

Conformation-dependent antibody response to Pseudomonas aeruginosa outer membrane proteins induced by immunization in humans

New Perspectives on Prevention and Management of Pseudomonas Aeruginosa Infections

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