Protection of trocar sites from gallbladder cancer implantation by
sodium hyaluronate carboxymethylcellulose-based bioresorbable
membrane (Seprafilm) in a murine model

Sasaki, Takuma; Shimura, H.; Tanaka, Takayuki; Nakashima, Kazunori; Matsuo, Katsuichi; Ikeda, Shuji

doi:10.1007/s00464-004-0079-0

Cited by 4 publications

(8 citation statements)

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“…These results suggest that neither type of film accelerates tumor cell adhesion or growth at the injured sites at least. The influence of conventional film on the peritoneal dissemination of tumor cells has been examined using animal experiments, and most have reported that conventional film does not affect tumor cell implantation . Similar to the present results, Pucciarelli et al reported that conventional film had no negative impact on the survival rate or tumor weight in nude mice that received peritoneal implantation of HT29 colorectal human tumor cells .…”

Section: Discussionsupporting

confidence: 84%

“…However, sodium hyaluronate, which is one of the component of conventional film, is an important extracellular matrix molecule for cell migration and has been reported to promote tumor cell growth . Therefore, the possibility that conventional film may accelerate the peritoneal dissemination of tumor cells has been argued both clinically and experimentally . Similarly, gelatin has been used recently as an useful scaffold for tissue regeneration because it is a degenerated product of collagen.…”

Section: Discussionmentioning

confidence: 99%

“…29 Therefore, the possibility that conventional film may accelerate the peritoneal dissemination of tumor cells has been argued both clinically and experimentally. 28,30 Similarly, gelatin has been used recently as an useful scaffold for tissue regeneration 22,23 because it is a degenerated product of collagen. Gelatin retains some informational signals, which may promote cell adhesion, differentiation and proliferation, such as the Arg-Gly-Asp sequence of collagen.…”

Section: Discussionmentioning

confidence: 99%

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The influences of a novel anti‐adhesion device, thermally cross‐linked gelatin film on peritoneal dissemination of tumor cells: Thein vitroandin vivoexperiments using murine carcinomatous peritonitis models

et al. 2017

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To create anti-adhesive materials to be more effective and safer, we developed a thermally cross-linked gelatin film that showed superior anti-adhesive effects with excellent peritoneal regeneration. However, it may act as a convenient scaffold for tumor cell growth, thereby accelerating peritoneal dissemination when used in surgery for abdominal tumors. In this study, we tried to clarify this issue using mouse carcinomatous peritonitis models. First, we examined the in vitro tumor cell growth of mouse B16 melanoma or Colon26 cells on the gelatin film or the conventional hyarulonate/carboxymethylcellulose film. Tumor cell growth on each film was significantly lower than that of the control (no film). Next, we conducted the following in vivo experiments: After the parietal peritoneum was partially removed and covered with each film or without any film, mice were inoculated intraperitoneally with B16 melanoma or Colon26/Nluc cells expressing NanoLuc luciferase gene. At 7 days after the operation, we measured the weight of B16 melanoma tumors or the NanoLuc activity of Colon26/Nluc cells using in vivo imaging at the injured sites. There were no significant differences in the weight of the tumors and the NanoLuc activity among the three groups. We also observed the survival time of mice receiving the same operation and treatments. There was no significant difference in the survival time among the three groups. These results suggest that the gelatin film will likely not accelerate peritoneal dissemination as a convenient scaffold for tumor cell growth when used in surgery for abdominal tumors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2122-2130, 2018.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The influences of a novel anti‐adhesion device, thermally cross‐linked gelatin film on peritoneal dissemination of tumor cells: Thein vitroandin vivoexperiments using murine carcinomatous peritonitis models

et al. 2017

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“…Of note, the excision of port sites in rats increased the likelihood of PSM in an experimental model (WAT-SON). Other investigators have applied some preventive measures, including peritoneal irrigation with povidoneiodine solution, in a murine model, as well as port-site protection with bio-reabsorbable materials (27,29,30). However, none could establish a firm relationship between laparoscopy and increased or decreased PSM frequency.…”

Section: Discussionmentioning

confidence: 99%

“…A cytology sampling was obtained, which did not influence therapeutic decisions. Mean exploration time was 22 minutes (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31). If the tumor was considered curable, the trocars were withdrawn and a laparotomy and radical gastrectomy were performed.…”

Section: Methodsmentioning

confidence: 99%

Absence of port-site metastases following staging laparoscopy for gastric carcinoma

Deogracias

Rodríguez‐Sanjuán

Torre

et al. 2006

Rev. esp. enferm. dig.

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Background: port-site metastases (PSM) have been reported following oncological laparoscopic surgery. However, their frequency after laparoscopic examination in gastric cancer has not been well established.Material and methods: prospective follow-up of 41 patients having had a staging laparoscopy and a follow-up longer than 12 months. Mean age was 65 years (29-89). After staging, an open gastrectomy was performed in 33 cases. Mean follow-up was 21.4 (12-66) months. PSM was defined as a node in the former portsite wound with adenocarcinoma histology at biopsy.Results: no patient showed clinical signs of PSM or port-site recurrence, even in advanced stages. We had no morbidity or postoperative mortality attributable to laparoscopic manoeuvres, and no need for laparotomy in cases without a gastrectomy indication.Conclusions: our results suggest that staging laparoscopy is a safe procedure in gastric carcinoma, as it is not associated with PSM after even considerable follow-up, and has a very low complication rate.

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Efficacy and Safety of Seprafilm for Preventing Postoperative Abdominal Adhesion: Systematic Review and Meta‐analysis

et al. 2007

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Protection of trocar sites from gallbladder cancer implantation by sodium hyaluronate carboxymethylcellulose-based bioresorbable membrane (Seprafilm) in a murine model

Cited by 4 publications

References 0 publications

The influences of a novel anti‐adhesion device, thermally cross‐linked gelatin film on peritoneal dissemination of tumor cells: Thein vitroandin vivoexperiments using murine carcinomatous peritonitis models

The influences of a novel anti‐adhesion device, thermally cross‐linked gelatin film on peritoneal dissemination of tumor cells: Thein vitroandin vivoexperiments using murine carcinomatous peritonitis models

Absence of port-site metastases following staging laparoscopy for gastric carcinoma

Efficacy and Safety of Seprafilm for Preventing Postoperative Abdominal Adhesion: Systematic Review and Meta‐analysis

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