Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Li, Yingdong; Ma, Yun‐Bao; Zhao, Jun; Zhao, Xinke

doi:10.1007/s11655-011-0897-6

Cited by 18 publications

(19 citation statements)

References 8 publications

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“…Ligusticum Chuanxiong prevents serum deprivation-induced PC12 cell apoptosis [32]. The extract from Danggui Buxue decoction, a subsidiary formula of BYHWD, protected the cardiomyocytes from H 2 O 2 -induced apoptosis through downregulation of caspase-3 expression [33]. Thus, the effects of these active anti-apoptotic components of BYHWD played an important role in preventing the ventricular remodeling through down regulation of AI.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that both Radix Angelicae Sinensis [31] and TPG [30] activated Bcl-2 and inhibited Bax expression both at gene and protein level, then the Bcl-2/Bax ratio was increased as a matter of course. In addition, extract from Danggui Buxue decoction [33] downregulated caspase-3 in the cardiomyocytes of the rat's heart. There was a decrease of Bax expression and caspase 3 activity.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Buyang Huanwu Decoction on Ventricular Remodeling and Differential Protein Profile in a Rat Model of Myocardial Infarction

Zhou

Liu

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Buyang Huanwu decoction (BYHWD) is a well-known and canonical Chinese medicine formula from “Correction on Errors in Medical Classics” in Qing dynasty. Here, we show that BYHWD could alleviate the ventricular remodeling induced by left anterior descending (LAD) artery ligation in rats. BYHWD treatment (18 g/kg/day) decreased heart weight/body weight (HW/BW), left ventricle (LV) dimension at end diastole (LVDd) and increased LV ejection fraction (LVEF) and LV fractional shortening (LVFS) significantly compared to model group at the end of 12 weeks. The collagen volume of BYHWD group was more significantly decreased than that of model group. Proteomic analysis showed that atrial natriuretic factor (ANF) was downregulated; heat shock protein beta-6 (HSPB6) and peroxiredoxin-6 (PRDX6) were upregulated in BYHWD-treated group among successfully identified proteins. The apoptotic index (AI) was reduced by BYHWD accompanied by decreased expression of Bax and caspase 3 activity, increased Bcl-2/Bax ratio, and phosphorylation of HSPB6 compared to that of model group. Taken together, these results suggest that BYHWD can alleviate ventricular remodeling induced by LAD artery ligation. The antiremodeling effects of BYHWD are conferred by decreasing AI through affecting multiple targets including increased Bcl-2/Bax ratio and decreased caspase 3 activity that might be via upregulated PRDX6, phosphorylation of HSPB6 and subsequently reduction of ANF.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Buyang Huanwu Decoction on Ventricular Remodeling and Differential Protein Profile in a Rat Model of Myocardial Infarction

Zhou

Liu

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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“…Extracts of AS are also commonly incorporated into dietary health supplements. According to recent studies, AS appears to possess anti‐cancer and anti‐oxidant activities . Despite our knowledge of these effects, the exact mechanism by which AS produces these pharmacological effects is unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of natural phytochemicals in Angelica sinensis (Danggui) on Nrf2‐mediated gene expression of phase II drug metabolizing enzymes and anti‐inflammation

Saw

et al. 2013

Biopharm & Drug Disp

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The root of Angelica sinensis (Oliv.) Diels (abbreviated as AS) (Danggui) has a long history in Asian herbal medicine. Recently, it was demonstrated that AS possesses anti-cancer and anti-oxidant activities. Because the transcription factor Nrf2 mediates the expression of many cellular anti-oxidative stress genes, including genes that are involved in phase II drug metabolism and anti-oxidative stress, this study sought to investigate whether pure compounds from AS or an AS extract could activate antioxidant response element (ARE)-mediated gene expression and induce anti-inflammatory activities. Z-Ligustilide (Ligu), 3-butylidenephthalide (Buty) and CO2 supercritical fluid-extracted lipophilic AS extract (SFE) were tested in HepG2-C8 cells stabilized with ARE luciferase reporter gene. Ligu and Buty caused significant toxicity only at 100 μm. All three samples induced ARE-luciferase activity; however, SFE at 8.5 µg/ml induced ARE-luciferase activity 2-3 fold more potently than did either of the pure compounds. SFE also significantly increased the endogenous mRNA of Nrf2 and the Nrf2 target anti-oxidative gene NAD(P)H dehydrogenase, quinone 1 (NQO1). The protein expression of NQO1 was also significantly induced by SFE. In RAW 264.7 cells, SFE suppressed lipopolysaccharide (LPS)-induced IL-1β and TNF-α expression about 2 fold stronger than sulforaphane, whereas both pure compounds and SFE suppressed inflammatory nitric oxide (NO) production. In summary, this study demonstrates that AS has anti-inflammatory effects and activates the Nrf2 pathway, which protects against oxidative stress.

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“…The most popular formulation is Danggui Buxue Tang (DBT) composed of Astragalus and Angelica at a 5 : 1 ratio, which is mainly used to treat anemia due to various causes via promoting hematopoiesis. 1) In recent years, studies have shown that DBT also has other pharmacological effects, such as enhancing immunity, 2) antagonizing diabetic nephropathy, 3) cardiovascular protection, 4) anti-fibrosis, 5,6) estrogen-like effect, 7,8) among others. Concerning the hematopoietic function of the AstragalusAngelica combination, most studies have revealed that Astragalus mixed with Angelica at 5 : 1 can strongly promote hematopoiesis.…”

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confidence: 99%

Effects of <i>Astragalus</i> Combined with <i>Angelica</i> on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

Tang

Chen

et al. 2017

Biological & Pharmaceutical Bulletin

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Danggui Buxue Tang (DBT), a combination of Astragalus and Angelica at a 5 : 1 ratio, mainly promotes hematopoiesis. However, in the clinic, the combination ratio of Astragalus and Angelica to treat low hematopoietic function is not an absolute 5 : 1 ratio, suggesting that the herbs may promote hematopoiesis better after being combined at a certain range of ratios. The objective of this study is to investigate the effect of different ratio combinations of Astragalus and Angelica on bone marrow hematopoiesis suppression induced by cyclophosphamide (CTX) and to probe the interaction and mechanism of Astragalus combined with Angelica in promoting hematopoiesis. Following establishment of the model, mice were administered with Astragalus (6.00 g·kg 1

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Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Abstract: The EDBD has protection on cardiomyocytes injured by H(2)O(2) through improving cell antioxidant ability, up-regulating hsp70 expression, and inhibiting caspase-3 activity.

Cited by 18 publications

References 8 publications

Effects of Buyang Huanwu Decoction on Ventricular Remodeling and Differential Protein Profile in a Rat Model of Myocardial Infarction

Effects of Buyang Huanwu Decoction on Ventricular Remodeling and Differential Protein Profile in a Rat Model of Myocardial Infarction

Effects of natural phytochemicals in Angelica sinensis (Danggui) on Nrf2‐mediated gene expression of phase II drug metabolizing enzymes and anti‐inflammation

Effects of <i>Astragalus</i> Combined with <i>Angelica</i> on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

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