Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

Valentão, Patrı́cia; Carvalho, Márcia; Fernandes, Eduarda; Carvalho, Félix; Andrade, Paula B.; Seabra, Rosa M.; Bastos, Maria de Lourdes

doi:10.1016/j.jep.2004.02.004

Cited by 18 publications

(34 citation statements)

References 12 publications

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“…•− through Fenton reactions, contributing to a series of pathological events characterized by reduction of the activities of antioxidant enzymes (Túnez et al, 2006;Valentão et al, 2004), lipid peroxidation (Jung et al, 2007;Palmer et al, 1988;Yang et al, 2007), induction of mitochondrial permeability transition (MPT) (Akao et al, 2003;Vercesi et al, 1997), Ca 2+ overload (Chu et al, 2008;Gen et al, 2001;Schomig et al, 1984), necrosis and apoptosis (Aikawa et al, 2002;Kuramochi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Ren

Qiao

Yang

et al. 2009

Phytotherapy Research

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Allium chinense, a traditional herbal medicine, has been used for the treatment of cardiovascular diseases for hundreds of years. In this study, A. chinense steroids (ACSs) including three steroidal glycosides and their parent aglycones were isolated from the bulbs of A. chinense. For the first time, their cardioprotective effects were evaluated in cultured rat cardiac H9C2 cells by pretreatment with ACSs for 24 h before exposure to 0.2 mm H(2)O(2). The results showed the cell viability decreased markedly when H9C2 cells were incubated with 0.2 mm H(2)O(2) alone for 2 h, while the cell lipid peroxidation (estimated by the excessive production of nitric oxide and malondialdehyde) and intracellular free calcium concentration ([Ca(2+)](i)) increased significantly. The addition of 20 microm (below the toxic concentration) of ACSs notably attenuated the cellular injury induced by H(2)O(2). The effects of ACSs in our experiments were similar to those of nimodipine, a clinically applied calcium channel blocker. Preliminary analysis of the structure-activity relationship indicated that ACSs with a spirostane-type skeleton exhibited stronger protection than that with a furostane-type skeleton, and glycosylation of the steroids could substantially lower the protective activities. The above results suggested the protective effects of steroids originated from A. chinense on the oxidative injury of H9C2 cells and ACSs may have potential for preventing cardiac injuries induced by oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Ren

Qiao

Yang

et al. 2009

Phytotherapy Research

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“…which is in consonance with our findings. [32][33][34] In view of the current findings, we propose that pretreatment with Hypericum extract can prevent adverse effects of hypobaric hypoxia. A more extensive research may lead to better understanding of interaction of Hypericum perforatum with major players of oxidative stress induced at high altitude.…”

Section: Discussionmentioning

confidence: 73%

Hypericum perforatum (ethanolic extract) ameliorates simulated hypobaric hypoxia induced oxidative stress and neuronal damage in brains of Balb/c mice

2018

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Background: Hypobaric hypoxia refers to lower oxygen availability at high altitudes and is the cause of high altitude illness. Drugs such as acetazolamide and dexamethasone provide symptomatic relief and are associated with undesired side effects. Plant extracts such as Hypericum perforatum, which are documented to have neuromodulatory role can be more beneficial in ameliorating high altitude illness. Aims and Objective: Progressive cognitive decline is the hallmark characteristic of hypobaric hypoxia induced neuropathology attributed to ensuing oxidative stress and subsequent hippocampal damage. We have explored the efficacy of ethanolic extracts of Hypericum perforatum in amelioration of hypobaric hypoxia induced oxidative stress and associated behavioral deficits in mice.Material and Methods: Male Balb/c mice were exposed to simulated altitude of 25,000 ft. for 7 days (6 hr. per day) in a specially designed chamber. Ethanolic extract of Hypericum perforatum (HPE)(25mg/kg of body weight) was given orally prior to hypoxia exposure and effects were compared to hypoxia and control groups.Results: Animals exposed to hypobaric hypoxia showed sign of cognitive deterioration at day 3 and day 7 in the Elevated Plus Maze and Passive Avoidance Step through behavioral paradigms as compare to normoxic animals. Administration of HPE was able to alleviate the amnesic effect in treatment group, indicated by reduction in transfer latencies at day 3(IR-3 = -0.66±0.07) and day 7 IR-7 = -0.81±0.06) in elevated plus maze task and increased passive avoidance step through latency at day 3, (IR-3 = 3.23±0.67),as compared to ±hypoxic mice. Hypoxia group of animals suffered significant oxidative stress compared to normoxic mice as indicated by up-regulated malondialdehyde and total nitrite levels in hippocampal homogenates. The plasma lactate dehydrogenase activity was also increased following hypoxia indicating tissue damage. Co-treatment with HPE in simulated hypobaric hypoxia insult for seven days resulted in significant reduction in malondialdehyde, total nitrites and plasma LDH levels in animals.Conclusion: Hypericum perforatum extract improves cognitive performance in hypobaric hypoxia exposed mice with a concomitant reduction in oxidative stress burden suggesting its plausible use for preventing high altitude illness.Asian Journal of Medical Sciences Vol.9(2) 2018 1-8

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“…[4][5][6][7] Polyphenols, and in particular flavonoids, are usually recognized as the compounds which are responsible for most of the plant extracts antioxidant activity. [8][9][10][11][12] The antidepressant activity of Hypericum perforatum (Guttiferae) 13 and evidence on the antioxidant activity of Hypericum species in vitro, [14][15][16][17][18][19][20][21][22] has encouraged us to further survey the chemical composition of several Hypericum species native to southern Brazil. Recently, some species were studied in our laboratory, affording a variety of phenolic compounds, such as benzopyrans, 23 benzophenones, 24 flavonoids 25 and phloroglucinol derivatives.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activityin Southern Brazil Hypericum Species

Bernardi

López-Alarcón

Aspée³

et al. 2008

J. Chil. Chem. Soc.

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The present study was conducted to assess the antioxidant activity of Hypericum species endemic to South Brazil, H. caprifoliatum, H. carinatum, H. myrianthum and H. polyanthemum. The free radical scavenging properties of plant extracts were evaluated employing different methodologies, including the bleaching of a stable free radical (2,2-diphenyl-1-picrylhydrazyl, DPPH) and the peroxyl reactivity indexes TRAP (Total Reactive Antioxidant Potential) and ORAC-pyrogallol red (Oxygen Radicals Absorbance Capacity). A fair correlation was found between total phenol content determined by Folin-Ciocalteau and DPPH consumption, both in crude methanol and n-hexane extracts. In particular, H. myrianthum and H. caprifoliatum showed the highest TRAP and ORACpyrogallol red values, respectively. This would imply that H. myrianthum contains a larger amount of antioxidants of lower reactivity.

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Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

Cited by 18 publications

References 12 publications

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Hypericum perforatum (ethanolic extract) ameliorates simulated hypobaric hypoxia induced oxidative stress and neuronal damage in brains of Balb/c mice

Antioxidant Activityin Southern Brazil Hypericum Species

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Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

Cited by 18 publications

References 12 publications

Protective effects of steroids from Allium chinense against H2O2‐induced oxidative stress in rat cardiac H9C2 cells

Protective effects of steroids from Allium chinense against H2O2‐induced oxidative stress in rat cardiac H9C2 cells

Hypericum perforatum (ethanolic extract) ameliorates simulated hypobaric hypoxia induced oxidative stress and neuronal damage in brains of Balb/c mice

Antioxidant Activityin Southern Brazil Hypericum Species

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Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells