Protective activity of mogroside V against ovalbumin‐induced experimental allergic asthma in Kunming mice

Abstract

We investigated the antiasthmatic effect of mogroside V (Mog V) in mice with ovalbumin (OVA)‐induced asthma. Administration of Mog V effectively attenuated OVA‐induced airway hyperresponsiveness and reduced the number of inflammatory cells in bronchoalveolar lavage fluid (BALF). Histological examination showed that Mog V reduced the inflammatory infiltration of the lungs in the asthmatic mice. ELISAs suggested that Mog V effectively decreased the levels of IL‐4, IL‐5, and IL‐13 in BALF and serum levels of OVA‐specific IgE and IgG1 in the asthmatic mice. A quantitative reverse‐transcription PCR assay also indicated that Mog V decreased the mRNA levels of IL‐17A, IL‐23, and RORγt in the lungs of the asthmatic mice (the opposite effect on Foxp3 mRNA). Furthermore, Mog V significantly reduced the OVA‐induced activation of NF‐κB in the lungs. This study indicates that Mog V alleviates OVA‐induced inflammation in airways, and this effect is associated with a reduction in NF‐κB activation. Practical applications A traditional Chinese medicine herb has been reported to have a strong curative effect on asthma in clinical practice. Siraitia grosvenorii is known in China as a functional food product with the ability to improve lung function. Mogroside V is a triterpene glycoside isolated from S. grosvenorii. Nonetheless, the antiasthmatic effect of mogroside V has not been evaluated yet. The aim of this study was to investigate the antiasthmatic activity of mogroside V in mice with chemically induced asthma. The data from this study will provide some scientific evidence supporting wider use of S. grosvenorii in functional foods.

Rubusoside alleviates the ovalbumin‐induced mice allergic asthma by modulating the NF‐κB activation

“…After Rbs administration, the inflammatory macrophages and eosinophils in the BALF of mice with asthma were notably reduced. In our previous study, we also observed that the inflammatory cells (i.e., lymphocytes macrophages and eosinophils) were decreased by Mogroside V (a triterpene glucoside) in the BALF of asthmatic mice (Song et al, 2019).…”

“…Thirty‐five mice were stochastically divided into the group of normal mice, group of OVA mice, or groups of OVA plus Rbs mice at 50, 150, or 300 mg Rbs/kg body weight ( n = 7 mice/group). Experimental asthma was induced in the mice using a previously described method (Song et al, 2019). The mice were sensitized by intraperitoneal injection of inducing reagents on Day 1 and Day 14.…”

“…Based on the 2 −ΔΔ C t formula (average of Δ C t control—Δ C t treated = ΔΔ C t ), we calculated the relative mRNA levels. The primer sequences used were identical to our previous work (Song et al, 2019).…”

Phytochemical composition and pharmacological impact of natural non-calorie sweetener- monk fruit (Siraitia grosvenorii): a review

“…Addition of mogroside V lowered the levels of different interleukin viz. IL-4, IL-5 and IL-13 in broncho alveolar lavage fluid (BALF) plus serum levels of ovalbumin (OVA)-induced inflammation in the asthmatic mice (Song et al, 2019). Mogrosides exhibited anti-inflammation properties in murine macrophage RAW 264.7 cells by down regulation of certain inflammatory genes such as iNOS, cyclooxygenase-2 COX-2 and interleukins (ILs) and upregulation of protective genes TRP53, PARP1, MAPK9 and BCL2l1.…”

Mogroside V Alleviates Lipopolysaccharide-Induced Neuroinflammation via Inhibition of TLR4-MyD88 and Activation of AKT/AMPK-Nrf2 Signaling Pathway

“…According to historical records, the fructus of Siraitia grosvenorii has several effects, such as profit lung, laxative, clear heat, and treatment of sore throat [ 18 ]. Moreover, according to numerous studies, Mogrosides also display multiple pharmacological effects on cancer, oxidative stress, inflammation, obesity, and diabetes [ 18 – 20 ]. Mog is one of the most effective components of Mogrosides.…”