Protective Activity of the Ethanol Extract of Cynanchum Paniculatum (Bunge) Kitagawa on Treating Herpes Simplex Encephalitis

Abstract

The first two authors contributed equally to this study To date there has been no valid treatment for herpes simplex encephalitis (HSV). This study explores the protective activity of ethanol extract of Cynanchum paniculatum (bunge) kitagawa for treatment of HSV. Cell models and animal models were established and divided into 4 groups: normal group, virus group, cynanchum paniculatum group and Dexamethasone group. Flow cytometry was employed to detect apoptosis of cell model and TUNEL assay was chosen to detect apoptosis of animal tissues. The survival time of the animal models was observed. ELISA was used to measure TNF-a expression and the Greiss method to measure Nitric Oxide (NO) expression in the mouse brain. As a result, it was found that extract of Cynanchum paniculatum can improve the survival rate of HSV-infected mice. The extract could prevent apoptosis in the neuron cell model and reduce apoptosis rate in brain tissue after HSV infection. With the extract intervention, TNF-a and NO levels in brain tissue were significantly decreased in the animal model. In conclusion, the extract of Cynanchum paniculatum can prevent HSVinducing impairment in the cell and animal model of HSE.Herpes simplex encephalitis (HSE) is a kind of viral encephalitis that attacks neonates as well as older children and adults (l) and is a significant cause of morbidity and mortality (2). Nowadays, the

Corilagin controls post-parasiticide schistosome egg-induced liver fibrosis by inhibiting Stat6 signalling pathway

“…The procedures for measuring ARG1 in the supernatant and PDGF, Fizz1, Ym1, TGFβ in serum were carried out according to our previous study (36, 37). Expression of ARG1 in the supernatant and PDGF, Fizz1, Ym1, and TGFβ in serum was determined by sandwich ELISA.…”

Cynanchum paniculatum and Its Major Active Constituents for Inflammatory‐Related Diseases: A Review of Traditional Use, Multiple Pathway Modulations, and Clinical Applications

“…[11,13]. e reason for this confusion may be due to the use of Cynanchum paniculatum radix for medicinal purposes; the whole plant was not recorded in detail by ancient herbology literature [11,[13][14][15]. As a single herbal medication, Xuchangqing was documented in Shennong Materia Medica for the treatment of treating hallucination and mental disorder, parasitic and virus, phytophthora, and malaria [1].…”

“…In view of its wide range of pharmacological activities, Cynanchum paniculatum has long been used as a medicinal plant in China. Ever since the first documentation of Xuchangqing as a top-grade medicine in Shennong Materia Medica, Cynanchum paniculatum was frequently cited in many other medical literatures, such as the Supplement to Recipes Worth A Thousand Gold (Qianjin Yi Fang, Tang Dynasty, AD 682), Taiping Holy Prescriptions for Universal Relief (Tai Ping Sheng Hui Fang, Song Dynasty, AD 992), and Compendium of Materia Medica (Ben Cao Gang Mu, Ming Dynasty, AD 1590) for the treatment of wind-damp syndrome, similar to other acute infectious diseases such as tuberculosis, acquired immune deficiency syndrome (AIDS), malaria, fevers, acute urinary infection, and abscesses [ 11 , 12 ]. However, there is a potential confusion on the source of Xuchangqing across ancient literature among Dysosma versipellis , Dicliptera chinensis , Cynanchum atratum , Cynanchum stauntonii , etc.…”

“…However, there is a potential confusion on the source of Xuchangqing across ancient literature among Dysosma versipellis , Dicliptera chinensis , Cynanchum atratum , Cynanchum stauntonii , etc. [ 11 , 13 ]. The reason for this confusion may be due to the use of Cynanchum paniculatum radix for medicinal purposes; the whole plant was not recorded in detail by ancient herbology literature [ 11 , 13 – 15 ].…”

“…[ 11 , 13 ]. The reason for this confusion may be due to the use of Cynanchum paniculatum radix for medicinal purposes; the whole plant was not recorded in detail by ancient herbology literature [ 11 , 13 – 15 ].…”