Protective and Curative Potentials of <i>Sida corymbosa</i> Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Abstract

Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl 4 ). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A 1 -H 4 ) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control-normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl 4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl 4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (P<0.05) in the treatment groups from 7-28 d, when compared with the negative control. Oxidative assay carried out showed that serum malondialdehyde (MDA) levels were significantly lower (P<0.05) in treatment groups than in negative control groups. These may be suggesting that the extract may have improved the antioxidant levels of the rats. Sida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl 4 .

Effects of Sida corymbosa Leaf-Extract on the Lipid Profile of Carbon Tetrachloride-Injected Male Rats

“…The LD50 of the ethanol-leaf extract of Sida corymbosa plant has been determined by Dike et al, [13], to be above 5000 mg/kgBW and confirmed according to [17] by administering 5, 000 mg/kgbw of the ethanol extract to each of two groups of one rat each. The observation was carried out on the rats for 1h after administration and 10 min for every 2 h interval for 24 h.…”

“…Sida corymbosa plants have been known in many parts of Africa to be used in treating diseases such as ulcers, liver problems, infertility, and dysentery [13][14]. Sida plants have over 200 species and many subspecies, among which is Sida corymbosa.…”