Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells

Akter, Reshmi; Kwak, Gi-Young; Ahn, Jung Yong; Mathiyalagan, Ramya; Ramadhania, Zelika Mega; Yang, Deok‐Chun; Kang, Se Chan

doi:10.3390/app12010236

Cited by 18 publications

(16 citation statements)

References 52 publications

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“…The free radical scavenging activity was evaluated using DPPH method with minor modifications to previous method ( Akter et al, 2021 ). 20 µl of extract and 180 µl of DPPH solution were added to a 96 well plate and then incubated in the dark for 30 min at 25°C, followed by vigorous shaking.…”

Section: Methodsmentioning

confidence: 99%

Pomegranate juice fermented by tannin acyl hydrolase and Lactobacillus vespulae DCY75 enhance estrogen receptor expression and anti-inflammatory effect

et al. 2022

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Phenolics are phytochemicals in plants, fruits, and vegetables have potential health-promoting efficacies. However, mostly available as a complex form. So, to increase the contents and nutritional value of the phenolic compounds, fermentation is most readily used in the food industry. Especially, the hydrolyzable tannins present in the pomegranate that can be liberated into monomolecular substances, which enhances biological activity. Thus, this study aims to convert hydrolyzable tannins to ellagic acid by fermentation using Tannin acyl hydrolase (TAH) and a novel bacteria strain Lactobacillus vespulae DCY75, respectively to investigate its effect on Estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) mRNA expression along with inflammation inhibition. As a result, the fermentation enhanced the ellagic acid content up to 70% by the synergetic effect of TAH and DCY75. Furthermore, fermented pomegranate (PG-F) increased cellular proliferation as well as upregulated the gene expression of estrogen regulators such as ERα, ERβ, and pS2 in breast cancer cell line (MCF-7), which commonly used to evaluate estrogenic activity. Moreover, to study the inflammation associated with low estrogen in menopause, we have analyzed the inhibition of nitric oxide (NO)/inducible nitric oxide synthase (iNOS) in RAW 264.7 cells. The PG-F juice did not exert any cytotoxicity in RAW 264.7 cells and inhibited NO production along with the downregulation of a major pro-inflammatory cytokine iNOS which indicates the anti-inflammatory potential of it. To sum it up, the fermented commercial pomegranate juice using a novel bacteria strain increased the amount of ellagic acid that the value added bioactive of pomegranate and it has significantly increased the estrogenic activity via upregulating estrogen related biomarkers expression and reduced the risk of related inflammation via NO/iNOS inhibition. This study could be a preliminary study to use fermented pomegranate as a potential health functional food after further evaluation.

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Section: Methodsmentioning

confidence: 99%

Pomegranate juice fermented by tannin acyl hydrolase and Lactobacillus vespulae DCY75 enhance estrogen receptor expression and anti-inflammatory effect

et al. 2022

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“…The reducing capacity of a compound indicates its potential antioxidant activity. To conduct this assay ( Akter et al, 2021 ), 100 µL of various concentrations of the samples were mixed with 250 µL of 0.2 mM phosphate buffer (pH 6.6) and 250 µL of 1% potassium ferricyanide. The mixtures were then incubated at 50 °C for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome expression profile of compound-K-enriched red ginseng extract (DDK-401) in Korean volunteers and its apoptotic properties

et al. 2022

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Ginseng and ginsenosides have been reported to have various pharmacological effects, but their efficacies depend on intestinal absorption. Compound K (CK) is gaining prominence for its biological and pharmaceutical properties. In this study, CK-enriched fermented red ginseng extract (DDK-401) was prepared by enzymatic reactions. To examine its pharmacokinetics, a randomized, single-dose, two-sequence, crossover study was performed with eleven healthy Korean male and female volunteers. The volunteers were assigned to take a single oral dose of one of two extracts, DDK-401 or common red ginseng extract (DDK-204), during the initial period. After a 7-day washout, they received the other extract. The pharmacokinetics of DDK-401 showed that its maximum plasma concentration (Cmax) occurred at 184.8 ± 39.64 ng/mL, Tmax was at 2.4 h, and AUC0–12h was 920.3 ± 194.70 ng h/mL, which were all better than those of DDK-204. The maximum CK absorption in the female volunteers was higher than that in the male volunteers. The differentially expressed genes from the male and female groups were subjected to a KEGG pathway analysis, which showed results in the cell death pathway, such as apoptosis and necroptosis. In cytotoxicity tests, DDK-401 and DDK-204 were not particularly toxic to normal (HaCaT) cells, but at a concentration of 250 μg/mL, DDK-401 had a much higher toxicity to human lung cancer (A549) cells than DDK-204. DDK-401 also showed a stronger antioxidant capacity than DDK-204 in both the DPPH and potassium ferricyanide reducing power assays. DDK-401 reduced the reactive oxygen species production in HaCaT cells with induced oxidative stress and led to apoptosis in the A549 cells. In the mRNA sequence analysis, a signaling pathway with selected marker genes was assessed by RT-PCR. In the HaCaT cells, DDK-401 and DDK-204 did not regulate FOXO3, TLR4, MMP-9, or p38 expression; however, in the A549 cells, DDK-401 downregulated the expressions of MMP9 and TLR4 as well as upregulated the expressions of the p38 and caspase-8 genes compared to DDK-204. These results suggest that DDK-401 could act as a molecular switch for these two cellular processes in response to cell damage signaling and that it could be a potential candidate for further evaluations in health promotion studies.

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“…The recommendation of vitamin C is 45 mg/day, and orange is one of the greatest sources, with 52 mg/100 g [35]. Many studies stress the high content of vitamin C in Kakadu plum, making it the greatest source of vitamin C in the world [32,[49][50][51], reaching 7000 mg/100 g FW [35]. Among Western fruits, the vitamin E average content is 0.3 mg/100 g, and cherry tomato is about 0.2 mg/100 g, while the content in some native fruits is much higher, such as quandong (Santalum acuminatum), with 0.7-4.78 mg/100 g DW; bush tomato (Solanum centrale), with 4.57 mg/100 g DW; and Kakadu plum (Terminalia ferdinandiana), with 6.08 mg/ 100 g DW [35].…”

Section: Nutritional Benefitsmentioning

confidence: 99%

Aboriginal Food Practices and Australian Native Plant-Based Foods: A Step toward Sustainable Food Systems

et al. 2023

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The current food system and food choices have resulted in the increased human use of natural resources such as water and soil, and have directly impacted the ‘Global Syndemic’—climate change, obesity, and undernutrition. Revitalising Indigenous food systems and incorporating native plant-based foods into current food systems may have the potential to reduce diet-linked chronic diseases and environmental degradation, and are important steps toward Indigenous rights and self-determination. This study aims to identify and describe Aboriginal food practices and Australian native plant-based foods and their social, environmental, and economic impacts on sustainable food systems. A scoping review was conducted using the five-stage framework informed by Arksey and O’Malley. To describe the results, the framework for sustainable food systems from the Food and Agriculture Organization of the United Nations—FAO was used. Articles were included if they described the impacts of Aboriginal food practices on sustainable food systems, were confined to studies that were conducted in the Australian context, and included native Australian plant-based foods. A total of 57 studies were identified that met the inclusion criteria. The major social impacts incorporated the nutritional and health benefits of Australian native plant-based foods, such as antidiabetic properties, anticancer and antioxidant activities, and cultural identification, involving Aboriginal ecological knowledge and their connection to their country. Within the environmental impacts category, studies showed that Australian native plant-based foods have environmental stress tolerance and some ecosystem benefits. The main economic impacts discussed in the literature were the source of income for remote communities and the potential market for Australian native plant-based foods. This review demonstrates that Aboriginal food practices and Australian native plant-based foods can contribute to more sustainable food systems and diets and give more voice and visibility to Aboriginal knowledge and aspirations. More research and investments are needed to face the challenges of including these foods in our current food systems.

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Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells

Cited by 18 publications

References 52 publications

Pomegranate juice fermented by tannin acyl hydrolase and Lactobacillus vespulae DCY75 enhance estrogen receptor expression and anti-inflammatory effect

Pomegranate juice fermented by tannin acyl hydrolase and Lactobacillus vespulae DCY75 enhance estrogen receptor expression and anti-inflammatory effect

Transcriptome expression profile of compound-K-enriched red ginseng extract (DDK-401) in Korean volunteers and its apoptotic properties

Aboriginal Food Practices and Australian Native Plant-Based Foods: A Step toward Sustainable Food Systems

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