Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats

Rajakumar, Thangarasu; Pugalendhi, Pachaiappan; Thilagavathi, Subbaiyan

doi:10.1007/s12291-017-0663-4

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…However, it should be considered that the increased mortality in most studies was caused by AITC applied as a volatile substance. Numerous positive effects were observed in other organisms after an oral ingestion of AITC or AITC-containing extracts possibly due to the intestinal metabolization of AITC (37)(38)(39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Effect of allyl-isothiocyanate on survival and antimicrobial peptide expression following oral bacterial infections in Drosophila melanogaster

Zimmermann,

Dähn,

Wagner

2024

Front. Immunol.

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Since infections with antibiotic-resistant bacteria cause increasing problems worldwide, the identification of alternative therapies is of great importance. Plant-derived bioactives, including allyl-isothiocyanate (AITC), have received attention for their antimicrobial properties. The present study therefore investigates the impact of AITC on survival and antimicrobial peptide (AMP) levels in Drosophila melanogaster challenged with the fly pathogenic bacteria Pectobacterium carotovorum subsp. carotovorum and Leuconostoc pseudomesenteroides. AITC, a sulfur-containing compound derived from glucosinolates, exhibits antimicrobial properties and has been suggested to modulate AMP expression. By using D. melanogaster, we demonstrate that AITC treatment resulted in a concentration-dependent decrease of survival rates among female flies, particularly in the presence of the Gram-negative bacterium Pectobacterium carotovorum subsp. carotovorum, whereas AITC did not affect survival in male flies. Despite the ability of isothiocyanates to induce AMP expression in cell culture, we did not detect significant changes in AMP mRNA levels in infected flies exposed to AITC. Our findings suggest sex-specific differences in response to AITC treatment and bacterial infections, underlining the complexity of host–pathogen interactions and potential limitations of AITC as a preventive or therapeutic compound at least in D. melanogaster models of bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Effect of allyl-isothiocyanate on survival and antimicrobial peptide expression following oral bacterial infections in Drosophila melanogaster

Zimmermann,

Dähn,

Wagner

2024

Front. Immunol.

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“…Increased levels of hexosamine, sialic acid, and hexose in plasma, liver, and mammary tissues were observed. Oral administration of AITC in DMBA-injected rats lowered the levels of glycoprotein components back to their original concentrations and implied the antimetastatic effects of AITC by inhibiting abnormal glycosylation [ 28 ].…”

Section: Aitc Anticancer Mechanismsmentioning

confidence: 99%

Anticancer Activity, Mechanism, and Delivery of Allyl Isothiocyanate

et al. 2022

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Allyl isothiocyanate (AITC) is a phytochemical that is abundantly present in cruciferous vegetables of the Brassicaceae family, such as cabbage, broccoli, mustard, wasabi, and cauliflower. The pungent taste of these vegetables is mainly due to the content of AITC present in these vegetables. AITC is stored stably in the plant as its precursor sinigrin (a type of glucosinolate), which is physically separated from myrosin cells containing myrosinase. Upon tissue disruption, myrosinase gets released and hydrolyzes the sinigrin to produce AITC and by-products. AITC is an organosulfur compound, both an irritant and toxic, but it carries pharmacological properties, including anticancer, antibacterial, antifungal, and anti-inflammatory activities. Despite the promising anticancer effectiveness of AITC, its clinical application still possesses challenges due to several factors, i.e., low aqueous solubility, instability, and low bioavailability. In this review, the anticancer activity of AITC against several cancer models is summarized from the literature. Although the mechanism of action is still not fully understood, several pathways have been identified; these are discussed in this review. Not much attention has been given to the delivery of AITC, which hinders its clinical application. However, the few studies that have demonstrated the use of nanotechnology to facilitate the delivery of AITC are addressed.

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“…Allyl isothiocyanate (AITC) is a phytochemical present in several dietary sources having various pharmacological properties (Zhang, 2010). Previously, we have reported the anti-oxidative, anti-in ammatory, and anti-cell proliferative properties of AITC in DMBAinjected rat mammary carcinoma through chemopreventive effects (Rajakumar et al 2015;Rajakumar et al 2018aRajakumar et al , 2018bRajakumar et al , 2018cThangarasu et al 2020). However, the action of AITC on the JAK/STAT signaling pathway of mammary cancer is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Allyl isothiocyanate inhibits invasion and angiogenesis in breast cancer via EGFR-mediated JAK-1/STAT-3 signaling pathway

Rajakumar

Pugalendhi

2023

Amino Acids

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Angiogenesis, invasion, and metastasis are the main events of cancer cells. JAK1/STAT3 is a key intracellular signaling transduction path, which controls the growth, differentiation, apoptosis, invasion, and angiogenesis in various cancer cells. The existing study explored the impact of AITC on the JAK-1/STAT-3 pathway in DMBAinjected rat mammary tumorigenesis. The mammary tumor was initiated through a single dose of 25 mg DMBA/rat through a subcutaneous injection administered near the mammary gland. We observed decreased body weight and augmented the total number of tumors, tumor incidence, tumor volume, well-developed tumor, and histopathological abnormalities in DMBA-injected rats and that were modulated after being treated with AITC. Staining of mammary tissues showed a high accumulation of collagen in DMBAtreated rats and it was normalized by the AITC treatment. Moreover, DMBA-induced mammary tissues showed up-regulated expressions of EGFR, pJAK-1, pSTAT-3, the nuclear fraction of STAT-3, and its associated products like VEGF, VEGFR2, HIF-1α, MMP-2, and MMP-9 and the down-regulated expressions of cytosolic STAT-3 and TIMP-2. Oral administration of AITC on DMBAtreated rats inhibits angiogenesis and invasion through modi ed these angiogenic and invasive markers. The nding of the current study is further con rmed by molecular docking analysis that shows a strong binding interaction between AITC with STAT-3 and cocrystal structure of STAT3 glide energy of -18.123 and − 72.246 (kcal/mole), respectively. Overall, the results suggested that AITC inhibits activation of the JAK-1/STAT-3 path, which subsequently prevents angiogenesis and invasion. It was recommended that AITC might develop a bene cial effect against breast cancer.

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Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats

Cited by 8 publications

References 25 publications

Effect of allyl-isothiocyanate on survival and antimicrobial peptide expression following oral bacterial infections in Drosophila melanogaster

Effect of allyl-isothiocyanate on survival and antimicrobial peptide expression following oral bacterial infections in Drosophila melanogaster

Anticancer Activity, Mechanism, and Delivery of Allyl Isothiocyanate

Allyl isothiocyanate inhibits invasion and angiogenesis in breast cancer via EGFR-mediated JAK-1/STAT-3 signaling pathway

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