Protective effect of Amphiroa dilatata on ROS induced oxidative damage and MMP expressions in HT1080 cells

Abstract

Antioxidative and matrix metalloproteinase (MMP) inhibitory effects of methanolic extract from ^ãéÜáêç~=Çáä~í~í~ were investigated in HT1080 cells. Treatment with ^K=Çáä~í~í~ extract suppressed the intensity of electron spin resonance signal on the generation of DPPH radicals in a cell-free system and intracellular reactive oxygen species (ROS) formation by 2′,7′-dichlorofluorescin diacetate (DCFH-DA). Radical simulated oxidation of membrane proteins and lipids were also inhibited by treatment with ^K=Çáä~í~í~ extract in a concentration-dependent manner. In addition, a protective effect on oxidative damage of purified genomic DNA was observed in the presence of ^K=Çáä~í~í~ extract. These results revealed that ^K=Çáä~í~í~ has an excellent scavenging ability on ROS-induced oxidative damage. Moreover, this extract reduced the expression levels of MMP-2 and -9 in gelatin zymography, and RT-PCR analysis without any cytotoxic influence. Therefore, ^K= Çáä~í~í~ has a promising potential as a valuable source to be used as ingredient in functional foods, related to oxidative damage. © KSBB hÉóïçêÇëW=Amphiroa dilatata, jjmJOI=jjmJVI=êÉ~ÅíáîÉ=çñóÖÉå=ëéÉÅáÉë= = = = =

Catechol-based matrix metalloproteinase inhibitors with additional antioxidative activity

“…The mixture was filtered through a Celite pad and concentrated in vacuo to give the title product as a white solid. 95 (s, 1H, OH), 12.34 (s, 1H, OH); 13 C NMR (CDCl 3 ): d ¼ 114.3, 116.1, 118.6, 119.0, 121.3, 125.5, 129.2, 136.4, 146.1, 149.3, 168 General procedure for the preparation of compounds 7-11…”

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Biological activities and health benefit effects of natural pigments derived from marine algae

“…In accordance with the epidemiological studies, in vivo studies using rodent model demonstrate protective effects of dietary kelp and other red and green algae against mammary carcinogenesis. In East Asian culture, marine algae have always been of particular interest (Khan, Kong, Kim, & Kim, 2010). Edible marine algae accounted for more than 10% of Japanese diet with an average consumption of 1.4 kg per person per year (Burtin, 2003).…”

Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells

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