Protective Effect of Antioxidant Vitamins on Red Blood Cell Lipoperoxidation induced by SO2 inhalation

Abstract

The aim of this research was to determine whether administration of antioxidant vitamins can reduce oxidant damage in erythrocytes induced by sulfur dioxide (SO2) inhalation. Meth- and sulfhemoglobin ratios, malonyldialdehyde (MDA) levels, osmotic fragility ratios and hematological parameters of a total of 28 rats were compared. SO2 was given at 10 ppm, 1 hour daily, for 45 days in a specially designed chamber. DL-alpha-Tocopherol acetate (40 mg/kg) and Na-ascorbate (200 mg/kg) treatments, initiated 3 days before SO2 exposures, were applied intraperitoneally 3 times a week for 45 days. Meth- and sulfhemoglobin ratios, MDA levels and osmotic fragility ratios were significantly higher in the SO2-treated group (p < 0.05). Significant decreases in MDA levels and osmotic fragility ratios were observed in the antioxidant-treated group (p < 0.05). SO2 inhalation resulted in higher MDA levels and osmotic fragility ratios, which can be reduced by vitamin E + C combination.

The level of maternal methemoglobin during pregnancy in an air-polluted environment.

“…The effect of SO 2 on the antioxidant status and lipid peroxidation of red blood cells and their reactions to ingested, inhaled, and parenterally administered sulfite have been described (Etlik et al 1997;Jenkins et al 2000). Inhalation of SO 2 causes bronchoconstriction (Lazarus et al 1997).…”

A review of the factors that influence erythrocyte osmotic fragility

“…Vitamin C (ascorbic acid) administration before transportation protected the erythrocyte membrane in pigs (Adenkola et al, 2010a, Adenkola et al, 2010b and goats (Minka & Ayo, 2010) transported by road for several hours. Vitamins C and E supplementation also reduced erythrocyte osmotic fragility and oxidative damage in rats (Etlik et al, 1997;Kraus et al, 1997) and chickens (Azeez et al, 2011a). In Gezel and Makoei breeds of sheep, increased vitamin E (α-tocopherol) levels associated with the increase in age caused a decrease in erythrocyte osmotic fragility (Asri-Rezaei et al, 2006).…”

Apricot sulfurization: An occupation that induces an asthma‐like syndrome in agricultural environments

“…Increased MDA levels were also shown in rats experimentally exposed to SO 2 inhalation. Etlik et al [1997] found significant decrease in MDA levels in rats which were pretreated with antioxidant vitamins. The occurrence of lipid peroxidation, therefore, may be considered as a new mechanism causing bronchoconstriction via changing membrane properties of bronchial smooth muscles or other cells in the airways of WIAS.…”