Protective effect of aspirin against mitomycin C-induced carcinogenicity, assessed by the test for detection of epithelial tumor clones (warts) in Drosophila melanogaster

Abstract

The present study assessed the protective effect of aspirin against carcinogenicity induced by mitomycin C (MMC) by the test for detection of warts/epithelial tumor clones in Drosophila melanogaster. Larvae were treated with different concentrations of aspirin alone (10, 20 or 40 mg/mL) or aspirin in association with MMC. MMC and ultrapure water were employed as the positive and negative control, respectively. Antioxidant activity was determined using the DPPH method. For performing cytotoxicity assay on HeLa cells, the aspirin concentrations used ranged from 200 mmol/L to 3,125 mmol/L. For assessment of apoptosis and necrosis, cells were incubated for 24 h with complete medium in the absence (control group) or presence of aspirin (12.5 mmol/L and 25 mmol/L). The results obtained in the assessment of the possible carcinogenic effects of aspirin at the three concentrations tested indicate no statistically significant increase in tumor frequency compared to the negative control. The anticarcinogenic activity assessment, where the larvae of D. melanogaster were previously induced to tumor formation by MMC and later treated with aspirin, showed a statistically significant reduction in the number of tumors compared to the positive control. Antioxidant activity across the three aspirin concentrations (10, 20 or 40 mg/mL) ranged from 20.81% to 26.5%. It was observed that aspirin reduced growth viability of HeLa cells in a concentration-dependent manner in comparison with the control. These results indicate that aspirin did not induce tumors in Drosophila and reduced MMC-induced carcinogenicity. The antioxidant activity and apoptosis induction appear to be the main mechanisms involved in reducing the frequency of tumors.

Study of the Toxic Manifestation of Etoposide the Concomitant Exposure of Acetyl Salicylic Acid As a Protector on the Example of C57Bl/6J Mice Line

“…Сделанные выводы, согласуются с результатами, полученными в 2018 г., в работе группы бразильских учёных, которые также занимались изучением протекторных свойств аспирина [10]. В результате проведённых исследований ими были выявлены антиоксидантные свойства ацетилсалициловой кислоты.…”

Bioinspired chitosan based functionalization of biomedical implant surfaces for enhanced hemocompatibility, antioxidation and anticoagulation potential: an in silico and in vitro study

“…96,97 Compared to CCDF and other specimens, commercially available synthetic anticoagulants heparin and aspirin exhibited lower antioxidant potential. Oliveira et al 98 reported that aspirin demonstrated less than 20% antioxidant activity as measured by the DPPH radical scavenging capacity assay. In contrast, CCDF specimen demonstrated more than 70% antioxidative potential.…”