Protective effect of astragalin and quercetin on ultraviolet (UV)-irradiated damage in HaCaT cells and Balb/c mice

Park, Ji Min; Cho, Jung-Keun; Mok, Ji Ye; Jeon, In Hwa; Kim, Hyeon Soo; Kang, Hyun Ju; Jang, Seon Il

doi:10.1007/s13765-012-2072-y

Cited by 10 publications

(8 citation statements)

References 10 publications

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“…Also, the reduction of these kinds of ingredients improves the galenic formulations and consequently could enhance the photoprotection adherence. Other natural compounds similar to the PLE extract used here may function as UV filters against induced damage in keratinocytes ( 20 ); some isoflavones, like genistein and daidzein, also block UVB induced skin burns in human and provide protection against photocarcinogenesis and photoaging ( 21 ). Other natural sunscreens are mycosporine-like amino acids synthetized by marine algae, fungi, and lichens.…”

Section: Discussionmentioning

confidence: 99%

Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations

et al. 2021

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Background: Novel approaches to photoprotection must go beyond classical MED measurements, as discoveries on the effect of UV radiation on skin paints a more complex and multi-pronged scenario with multitude of skin cell types involved. Of these, photoimmunoprotection emerges as a crucial factor that protects against skin cancer and photoaging. A novel immune parameter is enabled by the precise knowledge of the wavelength and dose of solar radiation that induces photoimmunosupression. Natural substances, that can play different roles in photoprotection as antioxidant, immune regulation, and DNA protection as well as its possible ability as sunscreen are the new goals in cosmetic industry.Objective: To analyze the effect of a specific natural extract from Polypodium leucotomos (PLE, Fernblock®), as part of topical sunscreen formulations to protect from photoimmunosuppression, as well as other deleterious biological effects of UV radiation.Methods: The possible sunscreen effect of PLE was analyzed by including 1% (w/w) PLE in four different galenic formulations containing different combinations of UVB and UVA organic and mineral filters. In vitro sun protection factor (SPF), UVA protection factor (UVA-PF), contact hypersensitivity factor (CHS), and human immunoprotection factor (HIF) were estimated following the same protocol as ISO 24443:2012 for in vitro UVA-PF determination.Results: PLE-containing formulations significantly reduced UV radiation reaching to skin. Combination of UVB and UVA filters with PLE increased SPF and UVAPF significantly. PLE also increased UV immune protection, by elevating the contact hypersensitivity factor and the human immunoprotective factor of the sunscreen formulations.Conclusion: This study confirms the double role of PLE in photoprotection. Together to the biological activity shown in previous works, the UV absorption properties of PLE confers a booster effect when it is supplemented in topical sunscreens increasing the protection not only at level of erythema and permanent pigment darkening but also against two photoimmunoprotection factors.

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Section: Discussionmentioning

confidence: 99%

Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations

et al. 2021

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“…Briefly, the dorsal parts of the mice were shaved using an electric shaver (Phillips, Malaysia). Mice from both groups (exposure and treatment groups) were then exposed to UVB irradiation for 3 min at a dose of 250 mJ/cm 2 on the 9th, 11th, and 13th days of treatment [21]. The source of irradiation was a 15 watt lamp emitting UV light with a wavelength of 312 nm (UVP, Upland, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Oral supplementation of L-glutathione prevents ultraviolet B-induced melanogenesis and oxidative stress in BALB/c mice

et al. 2019

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Dietary antioxidant supplements such as L-glutathione have gained considerable attention in dermatology and cosmeceutical fields. L-glutathione possesses antiaging, antimelanogenic, antioxidant, and anticancer properties. This study aimed to investigate the inhibitory effects of L-glutathione on melanogenesis activity and oxidative stress in ultraviolet B (UVB)-irradiated BALB/c mice. Eighteen female BALB/c mice were randomly divided into 3 groups: a control group (n=6), a group without UVB irradiation and L-glutathione administration; a UVB irradiated group (n=6), a group irradiated with a UVB dose of 250 mJ/cm2 for 3 min; and a treatment group (n=6), a group irradiated with UVB and treated with 100 mg/kg of L-glutathione by oral gavage. Treatment was given for 14 days, and UVB irradiation was given on days 9, 11, and 13. Oral L-glutathione significantly (P<0.05) reduced lipid peroxidation and elevated superoxide dismutase activity the and glutathione level. L-glutathione also inhibited melanin content and tyrosinase activity significantly (P<0.05) as compared with the UVB-irradiated group. Histopathological examination also showed that L-glutathione reduced the deposition of melanin pigment in the basal layer of the epidermis as compared with that in UVB-irradiated mice. All in all, the present study demonstrated that L-glutathione has the potential to be developed as a photoprotection agent against UVB-induced oxidative stress and melanogenesis.

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“…Animals were randomly divided into three groups: vehicle control group (n = 6), which were not exposed to UVB irradiation and not treated with resveratrol; exposure group (n = 6), which were exposed to UVB irradiation only; and treatment group (n = 6), which were exposed to UVB irradiation and also treated with resveratrol. The treatment was given for 14 successive days (Park et al, 2012).…”

Section: Animal Modelmentioning

confidence: 99%

“…The source of irradiation was a lamp with 312 nm of wavelength, 15 W (UVP, USA). Mice from both groups (exposure and treatment group) were exposed to UVB irradiation for 3 minutes at a dose of 250 mJ/cm 2 on 9th, 11th, and 13th day of treatment after shaving the dorsal part of the skin using an electric shaver (Phillips, Malaysia; Park et al, 2012).…”

Section: Uvb Irradiationmentioning

confidence: 99%

Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice

#Shelan#Nagapan

#Rohi#Ghazali²,

#Fredalina#Basri³

et al. 2018

J App Pharm Sci

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Commercial sunscreens contain cosmetic preservatives which could exert phototoxic effect when exposed to sunlight. Natural sources, such as resveratrol, have gain attention in the cosmetic world as it has higher antioxidant capacity than vitamins C and E. Resveratrol also possesses strong anti-inflammatory, anti-cancer, and anti-aging properties. It also has proven to reduce epidermal hyperplasia and skin thickness. The aim of the study was to evaluate the ameliorative effect of resveratrol in reducing epidermal hyperplasia of mouse skin exposed to UVB irradiation. Eighteen female BALB/c mice were randomly divided into three groups: control group (n = 6), without Ultraviolet B irradiation and resveratrol administration; UVB exposure group (n = 6), irradiated with UVB dose of 250 mJ/cm 2 for 3 minutes; and treatment group (n = 6), irradiated with UVB and treated with 0.02 ml of 200 mg/kg of resveratrol by oral gavage. Treatment was given for 14 days and UVB exposure was given on day 9, 11, and 13. On day 15, skin morphology was observed and skin-fold thickness was measured to evaluate edema. The mice were then sacrificed to obtain dorsal part of skin for histopathological observation using hematoxylin and eosin (H&E) stain. Resveratrol reduced skin scaling and erythema in UVB induced mice. Skin-fold thickness of resveratrol decreased significantly (p = 0.001) as compared to UVB irradiated group. H&E staining showed that resveratrol group reduced leukocyte infiltration and epidermal hyperplasia as compared to UVB exposure group. Hence, oral resveratrol was able to reduce skin thickness and epidermal hyperplasia and it has the potential to be developed as a natural alternative for photoprotection.

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Protective effect of astragalin and quercetin on ultraviolet (UV)-irradiated damage in HaCaT cells and Balb/c mice

Cited by 10 publications

References 10 publications

Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations

Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations

Oral supplementation of L-glutathione prevents ultraviolet B-induced melanogenesis and oxidative stress in BALB/c mice

Oral administration of resveratrol ameliorates epidermal hyperplasia in ultraviolet B irradiated BALB/c mice

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