Protective effect of berberine on beta cells in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats

Zhou, Jiyin; Zhou, Shiwen; Tang, Jun; Zhang, Kebin; Liu, Guang; Huang, Yaji; Xu, Ying; Ying, Yun; Zhang, Le; Li, Dandan

doi:10.1016/j.ejphar.2008.12.056

Cited by 179 publications

(117 citation statements)

References 42 publications

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“…Similar to Zhou et al, microscopic examination of endocrine pancreas revealed decreased β-cell mass, swelling of ER, and diminished secretory granules in β-cells, indicating β-cell failure and damage in HFD-STZ-treated rats (19). The data presented in this study suggest that SC treatment reduced inflammatory changes, prevented β-cell damage, and radically improved insulin secretory granules by averting β-cell damage and dysfunction attributable to glucose toxicity, lipotoxicity, and oxidative stress.…”

Section: Discussionmentioning

confidence: 52%

“…SOD and CAT are primary antioxidant enzymes involved in the direct scavenging of reactive oxygen species (28), and GSH-Px plays an essential role in the reduction of hydrogen peroxide. Together with the previous observation that diabetes is associated with increased lipid peroxidation and decreased antioxidant enzyme activity (19), we observed reductions in the activities of SOD, CAT, and GSH-Px as well as increased TBARS in HFD-STZ-induced diabetic rats. In accordance with a published study (29), SC treatment restored the activities of pancreatic antioxidant enzymes such as SOD, CAT, and GSH-Px to near normal levels and decreased lipid peroxidation, suggesting its anti-oxidant potential.…”

Section: Discussionmentioning

confidence: 61%

“…The specimens were then washed in phosphate buffer, dehydrated with graded acetone, and then embedded in araldite CY212 to make tissue blocks. Sections (70 -80 nm) were cut by an ultramicrotome (Ultracut E; Reichert AG, Vienna, Austria) and stained with uranyl acetate and lead acetate and examined under a transmission electron microscope (Morgagni 268 D; Fei Co., Eindhoven, The Netherlands) by a morphologist kept unaware of the treatment protocol (19).…”

Section: Ultrastructural Studies By Transmission Electron Microscopementioning

confidence: 99%

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Syzygium cumini Ameliorates Insulin Resistance and ^|^beta;-Cell Dysfunction via Modulation of PPAR^|^gamma;, Dyslipidemia, Oxidative Stress, and TNF-^|^alpha; in Type 2 Diabetic Rats

et al. 2012

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Abstract. Syzygium cumini (SC) is well known for its anti-diabetic potential, but the mechanism underlying its amelioration of type 2 diabetes is still elusive. Therefore, for the first time, we investigated whether SC aqueous seed extract (100, 200, or 400 mg/kg) exerts any beneficial effects on insulin resistance (IR), serum lipid profile, antioxidant status, and/or pancreatic β-cell damage in high-fat diet / streptozotocin-induced (HFD-STZ) diabetic rats. Wistar albino rats were fed with HFD (55% of calories as fat) during the experiment to induce IR and on the 10th day were injected with STZ (40 mg/kg, i.p.) to develop type 2 diabetes. Subsequently, after confirmation of hyperglycemia on the 14th day (fasting glucose level > 13.89 mM), diabetic rats were treated with SC for the next 21 days. Diabetic rats showed increased serum glucose, insulin, IR, TNF-α, dyslipidemia, and pancreatic thiobarbituric acid-reactive substances with a concomitant decrease in β-cell function and pancreatic superoxide dismutase, catalase, and glutathione peroxidase antioxidant enzyme activities. Microscopic examination of their pancreas revealed pathological changes in islets and β-cells. These alterations reverted to near-normal levels after treatment with SC at 400 mg/kg. Moreover, hepatic tissue demonstrated increased PPARγ and PPARα protein expressions. Thus, our study demonstrated the beneficial effect of SC seed extract on IR and β-cell dysfunction in HFD-STZ-induced type 2 diabetic rats.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 61%

Section: Ultrastructural Studies By Transmission Electron Microscopementioning

confidence: 99%

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Syzygium cumini Ameliorates Insulin Resistance and ^|^beta;-Cell Dysfunction via Modulation of PPAR^|^gamma;, Dyslipidemia, Oxidative Stress, and TNF-^|^alpha; in Type 2 Diabetic Rats

et al. 2012

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“…This mechanism may help to explain why metformin can help to prevent or postpone the onset of type 2 diabetes in atrisk subjects, as demonstrated in the Diabetes Prevention Program (Knowler et al 2002)-it may lessen the exposure of beta cells to the ectopic fat metabolites that promote beta cell failure (El-Assaad et al 2003;Zhou et al 2009;Gao et al 2011).…”

Section: Controlling Metabolic Syndrome and Diabetesmentioning

confidence: 99%

AMPK activation—protean potential for boosting healthspan

McCarty

2013

AGE

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AMP-activated kinase (AMPK) is activated when the cellular (AMP+ADP)/ATP ratio rises; it therefore serves as a detector of cellular "fuel deficiency." AMPK activation is suspected to mediate some of the health-protective effects of long-term calorie restriction. Several drugs and nutraceuticals which slightly and safely impede the efficiency of mitochondrial ATP g e n e r a t i o n -m o s t n o t a b l y m e t f o r m i n a n d berberine-can be employed as clinical AMPK activators and, hence, may have potential as calorie restriction mimetics for extending healthspan. Indeed, current evidence indicates that AMPK activators may reduce risk for atherosclerosis, heart attack, and stroke; help to prevent ventricular hypertrophy and manage congestive failure; ameliorate metabolic syndrome, reduce risk for type 2 diabetes, and aid glycemic control in diabetics; reduce risk for weight gain; decrease risk for a number of common cancers while improving prognosis in cancer therapy; decrease risk for dementia and possibly other neurodegenerative disorders; help to preserve the proper structure of bone and cartilage; and possibly aid in the prevention and control of autoimmunity. While metformin and berberine appear to have the greatest utility as clinical AMPK activators-as reflected by their efficacy in diabetes management-regular ingestion of vinegar, as well as moderate alcohol consumption, may also achieve a modest degree of healthprotective AMPK activation. The activation of AMPK achievable with any of these measures may be potentiated by clinical doses of the drug salicylate, which can bind to AMPK and activate it allosterically.

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“…Therefore, studies on type 2 diabetes formation with streptozotocin (STZ) injection following a high-energy diet were reviewed and the feeds used, energy contents, and foods that increase the energy contents were determined. The high-fat diet (HFD) administered to test animals in order to create obesity or insulin resistance varies depending on the study and the fat content changed between 22% and 60% (11)(12)(13). HFD content was prepared according to the average feed contents in studies using a standard diet and is given in Table 1.…”

Section: Preparation Of a High-fat Dietmentioning

confidence: 99%

The effect of acetylsalicylic acid on vasopressin, serum insulin levels, insulin resistance, and biochemical parameters in rats induced with experimental diabetes type 2

Şen¹,

Çeli̇k²

2017

Turk J Med Sci

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Background/aim: Acetylsalicylic acid (ASA) treatment in diabetic patients is very important owing to the increasing hyperactivity of thrombocytes and atherosclerosis. In several investigations, it was reported that diabetes caused increased coronary artery disease, cerebrovascular disease, and death. In this study, we aimed to investigate the effect of ASA on osmoregulation, glycemic control, and some biochemical parameters in rats induced with experimental diabetes type 2.Materials and methods: Twenty-four rats were randomly divided in four groups: control (I), ASA control (II), diabetic (III), and ASA diabetic (IV). Diabetes was induced by streptozotocin treatment (30 mg/kg, twice, intraperitoneal injection) in obese rats. ASA (150 mg/kg body weight, orally) was administered for 5 weeks in the ASA control and ASA diabetic groups. Serum electrolytes, creatinine, albumin, and total protein levels were analyzed with an autoanalyzer. Arginine vasopressin (AVP) and insulin were analyzed by ELISA techniques.Results: At the end of the study ASA treatments had decreased the fasting blood glucose levels but had interestingly increased the serum AVP levels in diabetics rats. Conclusion:AVP levels were increased 2-fold by ASA treatment in diabetic rats. For the first time in this study, the hypoglycemic effect of ASA was attributed to an increase in blood volume by AVP levels. This explanation may be a new approach to the literature on this topic.

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Protective effect of berberine on beta cells in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats

Cited by 179 publications

References 42 publications

Syzygium cumini Ameliorates Insulin Resistance and ^|^beta;-Cell Dysfunction via Modulation of PPAR^|^gamma;, Dyslipidemia, Oxidative Stress, and TNF-^|^alpha; in Type 2 Diabetic Rats

Syzygium cumini Ameliorates Insulin Resistance and ^|^beta;-Cell Dysfunction via Modulation of PPAR^|^gamma;, Dyslipidemia, Oxidative Stress, and TNF-^|^alpha; in Type 2 Diabetic Rats

AMPK activation—protean potential for boosting healthspan

The effect of acetylsalicylic acid on vasopressin, serum insulin levels, insulin resistance, and biochemical parameters in rats induced with experimental diabetes type 2

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