Protective Effect of Berberine on Expression Pattern of Apoptotic, Cell Proliferative, Inflammatory and Angiogenic Markers During 7,12-dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis

Manoharan, Shanmugam; Sindhu, Ganapathy; Mr, Nirmal; Vetrichelvi,; Balakrishnan, S.

doi:10.3923/pjbs.2011.918.932

Cited by 26 publications

(10 citation statements)

References 21 publications

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“…A positive association between loss of Bax immune positivity and shorter survival of patients with metastatic breast adeno carcinoma has been shown [22]. An inverse correlation between p53 and Bax immuno staining has been reported in oral carcinoma [23]. Our results support these observations.…”

Section: Discussionsupporting

confidence: 86%

Expression of apoptotic markers in patients with oral squamous cell carcinoma (OSCC)

Thomas¹,

Sethupathy²

2014

IOSRJDMS

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Section: Discussionsupporting

confidence: 86%

Expression of apoptotic markers in patients with oral squamous cell carcinoma (OSCC)

Thomas¹,

Sethupathy²

2014

IOSRJDMS

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“…Dysregulation of Bax expression has been reported in several precancerous and cancerous lesions of various cancers including oral cancer. [ 9 ] Bax serves as a proapoptotic protein and is downregulated in various carcinogenesis. [ 10 ]…”

Section: Introductionmentioning

confidence: 99%

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Manoharan¹,

Rajasekaran²,

Prabhakar³

et al. 2015

Toxicol Int

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Enicostemma littorale leaves are traditionally used for the treatment of several diseases, including inflammation and cancer. This study has taken effort to explore the antitumor initiating potential of E. littorale leaves (ElELet) by analyzing the expression pattern of apoptotic (p53, Bcl-2 and Bcl-2 associated X-protein), cell-proliferative (cyclin D1 and proliferating cell nuclear antigen), angiogenic (vascular endothelial growth factor), invasive (matrix metalloproteinase-2 and 9), and inflammatory (NF-κB and cyclooxygenase-2) markers during 7, 12-dimethylbenz (a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were induced in the buccal pouches of hamsters using the potent site and organ specific carcinogen, DMBA. DMBA application 3 times a week for 14 weeks resulted in tumor formation in the buccal pouches. Hundred percent tumor formations with dysregulation in the expression pattern of apoptotic, cell proliferative, inflammatory, angiogenic, and invasive markers were observed in the buccal pouches of hamsters treated with DMBA alone. ElELet at a dose of 250 mg/kg body weight orally to DMBA treated hamsters significantly prevented the tumor formation as well as corrected the abnormalities in the expression pattern of above mentioned molecular markers. ElELet thus modulated the expression pattern of all the above mentioned molecular markers in favor of the suppression of cell proliferation occurring in DMBA induced hamster buccal pouch carcinogenesis.

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“…The changes in histopathological status of this group and their timing were similar to other studies (30,31) . These observations could be attributed to the effects of DMBA as carcinogen which include formation of DNA adducts, induction of chronic inflammation, overproduction of ROS and oxidative DNA damage, there by leading to neoplastic transformation (32) . IHC staining of HBP mucosa GIIA with Bcl-2 antibody showed highly significantly increasing expression compared to normal group, there was highly significant difference between GI and GIIA where p value was <0.001.…”

Section: Discussionmentioning

confidence: 99%

“…These results are in agreement with those of other studies (12,14,15,39) . The increased level of Bcl-2 expression indicated that, inhibition of apoptosis by prevention the release of cytochrome C from mitochondria and promotion of carcinogenesis, While the decreased expression of Bak could be due to reduced apoptotic cell death as well as accelerated their growth (32,40) .…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Hydroxytyrosol as Chemopreventive Agent on Induced Hamster Buccal Pouch Carcinogenesis

Abd-Alwahab

Ahmed

Zouair

2019

Al-Azhar Assiut Dental Journal

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Aim: It was to investigate the efficacy of hydroxytyrosol (HT) as chemopreventive agent on induced hamster buccal pouch (HBP) carcinogenesis. Subjects and Methods: Forty Syrian male hamsters five weeks old, weighing 80-120g, were divided into three groups. GI: 10 animals were left untreated. GII: 10 animals were painted with 0.5% 7, 12-dimethylbenz(a)anthracene (DMBA) in paraffin oil, 3 times a week, for 8 weeks (GIIA) and14 weeks (GIIB). GIII: chemoprevention group, 20 animals in which HT was taken at week before as well as during the application of DMBA, 3 times a week on alternative days, for 8 weeks (GIIIA) and14 weeks (GIIIB). HPB mucosa were excised and trimmed to 1-2 cm average size, then processed for hematoxylin and eosin (H&E) stain and Immunohistochemical (IHC) staining utilizing Bcl-2 and Bak antibodies. Results: Gross observation revealed variable changes in the treated (G (s)), (GII and GIII) compared to that observed in GI. Histopathological findings revealed variations among the treated groups. IHC results regarding Bcl-2 expression revealed variability in the area percentage throughout the used groups. GI, GIIA, GIIB, GIIIA and GIIIB were (6.77٪, 31.58٪, 68.37٪, 16.07٪, 48.81٪) respectively, while IHC results regarding Bak expression revealed variability in the area percentage throughout the used groups. GI, GIIA, GIIB, GIIIA and GIIIB were (45.32%, 28.58%, 10.38%, 19.32%, 38.72%) respectively. Conclusion: HT is considered as a promising chemopreventive agent in decrease the incidence of HBP carcinogenesis in gross observation as well as in histological examination.

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Protective Effect of Berberine on Expression Pattern of Apoptotic, Cell Proliferative, Inflammatory and Angiogenic Markers During 7,12-dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis

Cited by 26 publications

References 21 publications

Expression of apoptotic markers in patients with oral squamous cell carcinoma (OSCC)

Expression of apoptotic markers in patients with oral squamous cell carcinoma (OSCC)

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Efficacy of Hydroxytyrosol as Chemopreventive Agent on Induced Hamster Buccal Pouch Carcinogenesis

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