Protective effect of betulinic acid on Freund's complete adjuvant‐induced arthritis in rats

Ding, Huimin; Chen, Hui; Shen, Guowei; Shouyun, Xiao; Junyang, Peng; Wei, Juncheng

doi:10.1002/jbt.22373

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…Furthermore, mairin inhibits the activation of the protein kinase B/ NF-κB pathway in TNFα-exposed RAFLSs, thereby alleviating RA-FLS proliferation, migration, and the inflammatory response. AA and CIA model experiments found that mairin inhibits paw swelling; arthritis index; joint pathology such as synovial tissue hyperplasia; cartilage destruction; vasospasm; inflammatory cytokines such as TNF-α, IL-1β, IL-6, IL-8, and IL-17A; and RA target proteins such as endothelial growth factor and transforming growth factor β (Mathew and Rajagoapl, 2017;Wang and Zhao, 2018;Huimin et al, 2019;Kun et al, 2020). These findings indicated that mairin has excellent therapeutic effects on RA and inhibits TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Identification of Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

et al. 2021

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Background: This study aimed to investigate the molecular mechanism of Radix Paeoniae Alba (white peony, WP) in treating immune inflammatory diseases of rheumatoid arthritis (RA) and tumor necrosis factor-alpha (TNF-α) inhibitors (TNFis) by using network pharmacology and molecular docking.Methods: In this study, the ingredient of WP and the potential inflammatory targets of RA were obtained from the Traditional Chinese Medicine Systematic Pharmacology Database, GeneCard, and OMIM databases, respectively. The establishment of the RA–WP-potential inflammatory target gene interaction network was accomplished using the STRING database. Network maps of the WP–RA-potential inflammatory target gene network were constructed using Cytoscape software. Gene ontology (GO) and the biological pathway (KEGG) enrichment analyses were used to further explore the RA mechanism and therapeutic effects of WP. Molecular docking technology was used to analyze the optimal effective components from WP for docking with TNF-α.Results: Thirteen active ingredients and 71 target genes were screened from WP, and 49 of the target genes intersected with RA target inflammatory genes and were considered potential therapeutic targets. Network pharmacological analysis showed that the WP active ingredients such as mairin, DPHCD, (+)-catechin, beta-sitosterol, paeoniflorin, sitosterol, and kaempferol showed better correlation with RA inflammatory target genes such as PGR, PTGS1, PTGS2, NR3C2, TNFSF15, and CHRM2, respectively. The immune-inflammatory signaling pathways of the active ingredients for the treatment of RA are the TNF-α signaling pathway, Toll-like receptor signaling pathway, cell apoptosis, interleukin-17 signaling pathway, C-type lectin receptor signaling pathway, mitogen-associated protein kinase, etc. Molecular docking results suggested that mairin was the most appropriate natural TNFis.Conclusion: Our findings provide an essential role and basis for further immune-inflammatory studies into the molecular mechanisms of WP and TNFis development in RA.

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Section: Discussionmentioning

confidence: 99%

Identification of Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

et al. 2021

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“…6 In RA, by secreting proinflammatory cytokines and mediators that drive angiogenesis, immune cell infiltration, and cartilage and bone destruction, macrophages are the key effector cells in the acute and chronic phases. 7 The amount of macrophages in the inflamed synovium increases owing to development of resident macrophages and infiltration through flowing monocytes. 8,9 Recent surveys indicate that mitogen-activated protein kinases (MAPKs) in macrophages are extremely activated and engaged in RA pathogenesis.…”

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confidence: 99%

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Huang¹,

Guo

Chitti³

et al. 2019

BioFactors

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Rheumatoid arthritis (RA) is a chronic and accelerated autoimmune illness with proliferative and damaging synovitis, resulting in joint death and cartilage and bone erosion. This study focused on the potential therapeutic effect of wogonin on complete Freund's adjuvant (CFA) induced RA in rats and the underlying mechanisms. Arthritis was experimentally caused in rats by subcutaneously injecting 0.1 mL of CFA into the subplantar area of the left hind paw under moderate anesthesia on day zero. The regular oral doses of indomethacin/wogonin began on day zero and proceeded after injection to day 35. Wogonin reduced arthritic score considerably, enhanced body weight, and reduced paw thickness. Wogonin also boosted red blood cell considerably along with hemoglobin and reduced white blood cell count and erythrocyte sedimentation rate. Wogonin substantially improved an altered level of oxidative stress markers, antioxidant proteins, and inflammatory cytokines in a dose‐dependent way. Wogonin inhibited p38 phosphorylation triggered by CFA and p65 nuclear translocation.

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“…Furthermore, mairin inhibits activation of the protein kinase B / NF-κB pathway in TNF-α exposed RA-FLS, thereby alleviating RA-FLSs proliferation, migration and in ammatory response. AA and CIA model experiments found that mairin inhibits paw swelling, arthritis index, joint pathology such as synovial tissue hyperplasia, cartilage destruction, vasospasm, in ammatory cytokines such as TNF-α, IL-1β, IL-6, IL-8, IL-17A, RA target proteins such as endothelial growth factor and transforming growth factor β [66][67][68][69] . These ndings indicated that mairin has excellent therapeutic effects on RA and inhibits TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Identification of Tumor Necrosis Factor-Alpha (Tnf-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

Zhou

2021

Preprint

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Background: This study aimed to investigate the molecular mechanism of Radix Paeoniae Alba (White peony, WP) in treating rheumatoid arthritis (RA) and tumor necrosis factor-alpha (TNF-α) inhibitor (TNFi) by using network pharmacology and molecular docking. Methods: In this study, the ingredient of WP and the potential targets of RA were obtained from the Traditional Chinese Medicine Systematic Pharmacology Database, GeneCard and OMIM databases, respectively. Establishment of RA-WP potential target genes interaction network using STRING database. Network maps of WP-RA-potential target genes network was constructed using Cytoscape software. gene ontology (GO), and biological pathway (KEGG) pathway enrichment analysis were used to further explore the RA mechanism and therapeutic effects of WP. Using molecular docking technology to analyze the optimal effective components from WP to docking with TNF-α. Results: 13 active ingredients and 71 target genes were screened from WP, 49 of which intersect with RA target genes and are considered as potential therapeutic targets.Network pharmacological analysis showed that the WP active ingredients of mairin, DPHCD, (+)-catechin, beta-sitosterol, paeoniflorin, sitosterol, and kaempferol showed better correlation with RA target genes such as PGR, PTGS1, PTGS2, NR3C2, TNFSF15, CHRM2. The signaling pathways of the active ingredients for the treatment of RA are TNF-α signaling pathway, toll-like receptor signaling pathway, cell apoptosis, interleukin-17 signaling pathway, C-type lectin receptor signaling pathway, mitogen-associated-protein kinase, etc. Molecular docking results suggest that mairin was the most appropriate natural TNFi.Conclusions: Our findings provide the essential role and basis, for further studies into the molecular mechanisms of WP and TNFi development in RA.

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Protective effect of betulinic acid on Freund's complete adjuvant‐induced arthritis in rats

Cited by 12 publications

References 23 publications

Identification of Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

Identification of Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Identification of Tumor Necrosis Factor-Alpha (Tnf-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking

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