Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Carreño, Ana Laura; Alday, Efrain; Quintero, Jael; Pérez, Lucía Franco; Valencia, Dora; Robles‐Zepeda, Ramón Enrique; Valdez-Ortega, Judith; Hernández, Javier; Velázquez, Carlos

doi:10.1016/j.jff.2016.12.008

Cited by 19 publications

(18 citation statements)

References 28 publications

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“…CAPE, an esterified derivative of caffeic acid, was used as an antiproliferative control compound, since it is a previously reported antiproliferative compound, as well as an effective antioxidant [11,15]. CAPE showed a reduction of M12.C3.F6 cell proliferation in 50% at a concentration of ~1 µM, suggesting for these data of CAPE, the low antiproliferative effect of Fe 2 PO and Fe 2 PC at 100 µM.…”

Section: Cellular Viability Assaymentioning

confidence: 99%

“…To evaluate the effect of Fe 2 PO and Fe 2 PC metallic complexes on the cellular redox state, we used the B-cell lymphoma M12.C3.F6 cell line as a model, due to its oxidative stress sensibility reflected on its cellular morphology [15]. We evaluated the amount of intracellular ROS in M12.C3.F6 cell line after incubation treatment with Fe 2 PO and Fe 2 PC metallic complexes, in addition to stress induction with H 2 O 2 , based on the methodology reported by Wang and Joseph [16], modified [17] and adapted to cell cytometry as reported by Eruslanov and Kusmartsev [4].…”

Section: Cellular Redox State Evaluationmentioning

confidence: 99%

“…(Table 2). CAPE and water were used as reference and dissolvent controls, respectively [15]. Data is expressed as mean of three independent experiments performed by triplicate Table 1: Chemical antioxidant activity of metallic complexes.…”

Section: Cellular Viability Assaymentioning

confidence: 99%

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Cellular Redox State Modifications Induced by Bioactive Fe(III)-Cyclophane Complexes Approaching to Selective Therapy Drug Design

Salazar-Medina¹,

Alday²,

Carreno³

et al. 2017

Med chem

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Previously we reported the capacity of the bio-inspired Fe(III) complexes, Fe 2 PO and Fe 2 PC, to mimic the activity of superoxide dismutase and peroxidase enzymes, as well as their capacity to reduce the cytotoxicity generated by superoxide radicals in human peripheral blood mononuclear cells, under stress conditions. Based on those findings, we decided to evaluate the cytotoxicity, antioxidant and redox state modulation capacity of Fe 2 PO and Fe 2 PC complexes, pondering them as drug candidates against free radicals unbalance disorders. Fe 2 PO and Fe 2 PC deactivated the ABTS synthetic radical with an IC 50 value of 38.4 ± 0.9 µM and 28.9 ± 0.2 µM, respectively, while against the DPPH radical, the IC 50 value was over the higher concentration tested (>200 µM) for both complexes. As desirable for any drug candidate, none of the metallic complexes (at 25, 50 and 100 µM) induced cytotoxicity on M12.C3.F6 cells (a murine B-cell lymphoma model), but differences in the redox state modulation were observed on the basis of fluorescence detection of a 2′,7′-dichlorofluorescin probe by flow cytometry. Cells under normal conditions and preincubated with Fe 2 PO and Fe 2 PC complexes slightly augmented the reactive oxygen species concentration, meanwhile, cells under stress condition preincubated with H 2 O 2 and metallic complexes, showed a higher augmentation in the reactive oxygen species concentration, in comparison to the controls. Finally, a cellular internalisation assay was performed, showing that Fe 2 PO and Fe 2 PC exert those effects from the outside of the cells. All these results suggest the ability of Fe 2 PO and Fe 2 PC complexes to selectively increase the reactive oxygen species concentration in cells with a free radical unbalance, without inducing mortality in cells under normal conditions.

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Section: Cellular Viability Assaymentioning

confidence: 99%

Section: Cellular Redox State Evaluationmentioning

confidence: 99%

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Cellular Redox State Modifications Induced by Bioactive Fe(III)-Cyclophane Complexes Approaching to Selective Therapy Drug Design

Salazar-Medina¹,

Alday²,

Carreno³

et al. 2017

Med chem

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“…Caffeic acid phenethyl ester (CAPE) has been identified as the main active component of propolis and has been reported to possess various pharmacological activities, including anti-oxidant, anti-inflammatory, anti-cancer, anti-fibrosis, anti-proliferation and anti-bacterial properties [ 34 , 35 ]. It has been reported that CAPE attenuates fibrosis via inhibiting the TGF-β1/Smad3 pathway; in addition, CAPE can lower the expression levels of renal inflammatory chemokines and reversed the levels of SOD and MDA [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

CAPE-pNO2 ameliorated diabetic nephropathy through regulating the Akt/NF-κB/ iNOS pathway in STZ-induced diabetic mice

Wang

Fan

et al. 2017

Oncotarget

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Diabetic nephropathy (DN) is one of the most severe complications of diabetes mellitus. This study aimed to determine the effects and potential mechanism of caffeic acid para-nitro phenethyl ester (CAPE-pNO2), a derivative of caffeic acid phenethyl ester (CAPE), on DN; In vivo, intraperitoneal injections of streptozotocin (STZ) were used to induce diabetes in mice; then, the mice were intraperitoneally injected daily with CAPE or CAPE-pNO2 for 8 weeks. The mice were sacrificed, and blood samples and kidney tissues were collected to measure biological indexes. The results showed that CAPE and CAPE-pNO2 could lower serum creatinine, blood urea nitrogen, 24-h albumin excretion, malondialdehyde and myeloperoxidase levels and increase superoxide dismutase activity in diabetic mice. According to HE, PAS and Masson staining, these two compounds ameliorated structural changes and fibrosis in the kidneys. In addition, the immunohistochemical and western blot results showed that CAPE and CAPE-pNO2 inhibited inflammation through the Akt/NF-κB pathway and prevented renal fibrosis through the TGF-β/Smad pathway. In vitro, CAPE and CAPE-pNO2 inhibited glomerular mesangial cell (GMC) proliferation, arrested cell cycle progression and suppressed ROS generation. These compounds also inhibited ECM accumulation via regulating the TGF-β1, which was a similar effect to that of the NF-κB inhibitor PDTC. More importantly, CAPE and CAPE-pNO2 could up-regulate nitric oxide synthase expression in STZ-induced diabetic mice and HG-induced GMCs. CAPE-pNO2 had stronger effects than CAPE both in vivo and in vitro. These data suggest that CAPE-pNO2 ameliorated DN by suppressing oxidative stress, inflammation, and fibrosis via the Akt/NF-κB/ iNOS pathway.

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“…Treated with CAPE, tomato fruits can be stored for 20 days at 25°C. Carreno et al (2017) evaluated the cellular antioxidant activity (CAA) of CAPE, and found that CAPE has a cellular protective effect against reactive oxygen species (ROS). This study is aimed at evaluating the effects of different concentrations of caffeic acid on maintenance of postharvest fruit quality and to extend the shelf life of mulberry fruit through comprehensive analysis of several physiological parameters in the treated fruits with those in the control.…”

Section: Introductionmentioning

confidence: 99%

Caffeic acid as a preservative that extends shelf-life and maintains fruit quality of mulberries during cold storage

Zhang¹,

Kang²,

Liu³

et al. 2018

Afr. J. Agric. Res.

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Fruits can be easily infected and damaged by microbes. Cold storage is a popular approach used to extend the shelf-life of fruits. In this paper, the effect of caffeic acid on physiological parameters and shelf-life of mulberries (Morus alba L.) stored for 21 days at 4°C was evaluated. The results showed that the shelf-life was significantly improved in the mulberries treated with the different concentrations of caffeic acid solution for 5 min (P < 0.05). Certain physiological parameters, like phenolics, anthocyanins, flavonoids and Vitamin C were also significantly increased (P < 0.05) in the treated mulberries. The results showed that the rotting rate and the weight loss ratio were 47.0 and 6.6% in the 0.20 g/L caffeic acid-treated fruits after storing for 21 days at 4°C, respectively. While these two parameters were 79.0 and 9.7% in the control. The malondialdehyde (MDA) content was significantly lower (P < 0.05) in the 0.20 g/L caffeic acid-treated mulberries than that in the samples treated with 0.00, 0.10, 0.25 and 0.30 g/L caffeic acid. Moreover, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities in the caffeic acid treated mulberries were significantly higher than those in the control (P < 0.05). Therefore, caffeic acid, as a preservative, is favorable for elongation of the shelf-life, maintenance of the quality and inhibition of fruit decay in mulberries. This study is greatly informative to mulberry growers and commercial sellers.

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Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Cited by 19 publications

References 28 publications

Cellular Redox State Modifications Induced by Bioactive Fe(III)-Cyclophane Complexes Approaching to Selective Therapy Drug Design

Cellular Redox State Modifications Induced by Bioactive Fe(III)-Cyclophane Complexes Approaching to Selective Therapy Drug Design

CAPE-pNO2 ameliorated diabetic nephropathy through regulating the Akt/NF-κB/ iNOS pathway in STZ-induced diabetic mice

Caffeic acid as a preservative that extends shelf-life and maintains fruit quality of mulberries during cold storage

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