Protective effect of caffeine on Ethyl methanesulfonate-induced wing primordial cells of Drosophila melanogaster

Abstract

Objectives:The antimutagenic effect of caffeine is evaluated against ethyl methanesulfonate (EMS)-induced mutation rate in Drosophila.Materials and Methods:The mutation rate is evaluated using wing mosaic assay. In transheterozygous larvae, multiple wing hair (mwh 0.3-3) and flare (flr 3-38.8) genes were used as markers of the extent of mutagenicity.Results:The results at 0.5 and 1.0 mM EMS concentration at both 48 ± 4 and 72 ± 4 h have shown consistent increase in mutation rate, which was being measured as frequency of clone formation per 105 cells. Toxicity of caffeine at 5 mM concentration was parallel to that of distilled water alone. At 0.5 mM EMS concentration at 42 ± 4 and 72 ± 4 h, Drosophila larvae mutation rate was significantly increased. Although caffeine prevented mutation rate in all pre, post, and combined treatment, it was more significant in pretreatment experiments where it was found to be effective in reducing the genotoxicity of EMS. However, the concentration of caffeine as recommended in dietary allowance did not induce the frequency of mutant clones in somatic mutation and recombination test (SMART) recorded.Conclusion:This study shows that caffeine significantly reduced the genotoxicity induced by EMS. However, the limitation in completely abolishing genotoxicity induced by EMS as observed at the dietary allowance of caffeine makes it interesting for further in-depth study. Further studies on the molecular mechanism of antigenotoxic effect of caffeine will also be interesting.

In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

“…The different IP values of 166.67% and 96.93% for CCC and CFCC, respectively, at the lowest tested concentration could be due to the CAF content in CCC (0.016 mM CAF) since CFCC does not consist of CAF. This is in agreement with several reports showing CAF antigenotoxic capacity against X-rays [ 72 , 73 ] and ethyl methanesulfonate (SMART assay [ 74 ] and yeast (15 mM) [ 75 ]). The IP value of CCC at 100 mg/mL decreased up to 98.88% and this fact could be due to the absence of antigenotoxicity observed in the highest CAF concentration.…”