Protective effect of calycosin-7-O-β-D-glucopyranoside against oxidative stress of BRL-3A cells induced by thioacetamide

Abstract

Background:Calycosin-7-O-β-D-glucopyranoside (CG) is a natural isoflavone found in traditional Chinese medicines Astragali Radix (AR).Objective:Calycosin-7-O-β-D-glucopyranoside, an isoflavone isolated from AR, has been found to have potent antioxidantive effects. This study was designed to investigate whether CG prevents oxidative stress induced by thioacetamide (TAA).Materials and Methods:BRL-3A cells were pretreated with different concentrations of CG (10, 20, 40 mg/mL) for 12 h and then exposed to 0.18 mol/L TAA for 2 h. The cell viability were examined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium assay, total antioxidant capacity, malondialdehyde (MDA) and the activity of antioxidant enzymes, including catalase, glutathione peroxidase and superoxide dismutase were determined by microplate method. Reactive oxygen species (ROS) generation was quantified by the 2’,7’-dichlorofluorescin-diacetate method. Protein and mRNA expression of CYP2E1 were determined by western blotting and real-time PCR.Results:The cell oxidative stress was significantly increased after 2 h of TAA exposure. Pretreatment of BRL-3A cells with CG significantly increased the activities of antioxidant enzymes, scavenged ROS and reduced MDA production. CG decreased the expression of CYP2E1, and ultimately decreased TAA-induced BRL-3A cells oxidative stress.Conclusions:Calycosin-7-O-β-D-glucopyranoside has a protective effect against TAA-induced oxidative stress in BRL-3A cells, and that the underlying mechanism involves in scavenging of ROS and the modulating expression of CYP2E1.

The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury

“…Calycosin, a major isoflavonoid in Radix Astragali Mongolici , was reported to exhibit anti-oxidative, anti-inflammatiry, tumor suppressive and osteogenic properties 17 – 20 . Calycosin also was demonstrated to ameliorate diabetes-induced cognitive impairments via PI3K/Akt/GSK-3β signaling pathway 21 .…”

A review of the botany, phytochemistry, traditional uses, pharmacology, toxicology, and quality control of the Astragalus memeranaceus

“…FMN and CAL, two important isoflavones in Huangqi, have been widely studied for their multiple pharmacological functions ( Gao et al, 2014 ). CG has been demonstrated to possess various pharmacological activities, including antioxidant, anti-inflammatory, and neuroprotective activities ( Choi et al, 2007 ; Jian et al, 2015 ). More importantly, CG has been described as a chemical indicator for the quality control of Huangqi in the Chinese Pharmacopoeia (2020).…”

Mechanism and Progress of Natural Products in the Treatment of NAFLD-Related Fibrosis

“…Calycosin (Figure 7A) is a phytoestrogen with a similar structure to mammalian estrogen that is extracted from the root of Astragalus membranaceus. Pharmacological research and clinical practice have proven that calycosin improves triglyceride metabolism and antioxidant free radicals, inhibits liver injury, regulates glucose uptake disorders in hepatocytes, and inhibits HCC [76,77,[136][137][138]. Overexpression of ERβ or calycosin alone inhibited the proliferation and migration of LX-2 cells induced by TGF-β1, downregulated α-SMA, PC-I, TIMP-1, and p-STAT3 and upregulated the protein expression of matrix metalloproteinase (MMP)-1.…”