Protective effect of Caralluma fimbriata against high-fat diet induced testicular oxidative stress in rats

Gujjala, Sudhakara; Putakala, Mallaiah; Gangarapu, Venkatanarayana; Nukala, Srinivasulu; Bellamkonda, Ramesh; Rajendran, R.; Desireddy, Saralakumari

doi:10.1016/j.biopha.2016.06.031

Cited by 31 publications

(13 citation statements)

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“…Many previous studies have proved the fact that HFD exposure can result in testicular histopathological lesions and decreased reproductive organ weights. [8][9][10][11][12][13][14] In the present study, HFD-induced testicular damage was supported by the decrease in relative weights of tests, sperm parameters, low levels of serum T, which may be consequences of elevated oxidative stress and excessive apoptosis in the testes. However, the adverse alterations induced by HFD exposure were alleviated by GSPE treatment.…”

Section: Discussionmentioning

confidence: 63%

“…Low levels of testosterone (T) and varying degrees of testicular dysfunction have been demonstrated in obese men and male animals, with oxidative stress and testicular apoptosis playing important roles in affecting testicular function. [6][7][8][9][10][11][12][13][14] The involvement of oxidative stress in male reproductive dysfunction has been well documented. [15][16][17] Enhanced oxidative stress and decreased antioxidant capacity are considered as having deep impact on the pathogenesis of obesity induced male reproductive damage.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 Fortunately, previous laboratory animal studies have supported the protective role of antioxidants against obesity induced male reproductive function damage. 8,19 With regard to the undesirable consequences of obesity on male reproductive system, nding efficient protective pharmacological agents are of great therapeutic interest.…”

Section: Introductionmentioning

confidence: 99%

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Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Wang

et al. 2019

RSC Adv.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Wang

et al. 2019

RSC Adv.

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“…Several papers are available, which report a significant decrease in testicular weight of rats fed with a HFD (Yan et al 2015, Gujjala et al 2016, beside those reporting no significant difference in testicular mass (Hu et al 2013, Campos-Silva et al 2015. A previous study (El-Desoky et al 2013) stated that administration of SP, suspended in water, by gavage for 60 consecutive days did not cause any significant difference in testis weights.…”

Section: Discussionmentioning

confidence: 99%

Spirulina Platensis Affects Factors Involved in Spermatogenesis and Increases Ghrelin Receptors in Testis Tissue of Rats Fed a High-Fat Diet

Esener

Gürel-Gürevin

Isbilen-Basok

et al. 2017

Polish Journal of Veterinary Sciences

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Ghrelin is a peptide hormone which plays important role in maintaining growth hormone release and energy homeostasis in vertebrates. Spirulina platensis (SP) has antioxidant and hypolipidemic effects due to its ingredients. In this study we aimed to investigate the effects of SP on the testicular structure and relation between ghrelin and testosterone in the testis of rats fed a high fat diet (HFD). Sixty four young adult male rats were used and divided to 8 equal groups. Experimental groups received addition of 10% cholesterol (CHL), 43% hydrogenated vegetable oil (HVO) and 3% SP alone or in combination to basal diet while the control group received only basal diet. Serum ghrelin and testosterone levels were measured with ELISA. Receptors for ghrelin and androgen were detected with immunohistochemistry. For histomorphometric investigation, tubulus seminiferus, intertubular area, tubulus seminiferus lumen, Leydig cell nucleus, Sertoli cell nucleus, germ cell nucleus, spermatocyte nucleus and elongated spermatid volume densities were determined stereologically. Serum ghrelin level was increased especially in HVO and CHL combination group compared to the control while serum ghrelin levels were close to control levels in SP-received groups. Ghrelin receptor level was increased in tubulus seminiferus with HVO+CHL administration but this effect was, however, limited in HVO+CHL and SP challenged groups. HVO+CHL administration caused a significant decrease in Leydig cell nucleus volume density, as well as in all SP-received groups, compared to the control. Significantly increased spermatocyte nucleus volume density in cholesterol-receiving groups was decreased to control level with SP alone and its combinations.

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“…[ 2 ] Several members of genus Caralluma have found various medicinal uses: antidiabetic, antihyperglycemic, antiparasitic, antitrypanosomal, antiulcer, neuroprotective, antipyretic, anti-inflammatory, antinocicepetive, antioxidant, antiobesogenic, and antiartherosclerotic properties. [ 3 4 5 6 7 8 9 10 11 12 ] This genus has been extensively explored for a variety of pharmacological activities as compared with other species, but not all species were tested for their biological activity. Locally known as “Daghmous,” “Zakkum,” or “Tikiwt”, CE is a plant species communally used in Moroccan traditional medicine for their presumed anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Toxicological evaluation of the aqueous extract of Caralluma europaea and its immunomodulatory and inflammatory activities

et al. 2017

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Background:Caralluma europaea (CE) has been studied for its chemical constituents, and no information is available on its toxicity or its pharmacological activities.Objective:To determine the toxicity of an aqueous extract of CE stems in vitro and in vivo after acute and subchronic oral gavages in Swiss albino's mice and its immunomodulatory and inflammatory activities.Materials and Methods:The extract was administrated in single oral dose at 5 g/kg body weight for the acute toxicity test and by gavages daily at doses of 1, 2.5, or 5 g/kg for 30 consecutive days for the subchronic toxicity test. The immunomodulatory activities and inflammatory activities were tested by the evaluation of hemagglutination antibodies (HAs) titers and delayed-type hypersensitivity (DTH) response.Results:For the dose of 1 g/kg, no visible toxic effects were observed. However, for the higher doses, clinical observations of toxicity were noted after 1 week of treatment. This was confirmed by the biochemical parameters values and the histology analyses of the spleen, liver, and kidney tissues. The high cellular mortality rate in vitro when treated with CE extract confirmed their toxicity potential. There was also increase of “HA titer” and “DTH” response in mice treated with nontoxic dose of CE (1 g/kg) compared to control group. This immune activity was confirmed by the high number of lymphocytes infiltrates noted in the different organs.Conclusion:We conclude that CE at the dose up of 1 g/kg produced toxic effect in mice that induced an immune inflammatory reaction.SUMMARY Caralluma europaea (CE) has been studied for its chemical constituents, and no information is available on its toxicity or its pharmacological activities. The objective is to determine the toxicity of an aqueous extract of CE stems in vitro and in vivo after acute and subchronic oral gavages in Swiss albino's mice and its immunomodulatory and inflammatory activities. For the dose of 1 g/kg, no visible toxic effects were observed. However, for the higher doses, clinical observations of toxicity were noted after 1 week of treatment. This was confirmed by the biochemical parameters values and the histology analyses of the spleen, liver, and kidney tissues. The high cellular mortality rate in vitro confirmed their toxicity potential. There was also increase of “hemagglutination antibody titer” and “delayed-type hypersensitivity” response in mice treated with nontoxic dose of CE (1 g/kg) compared to control group. This immune activity was confirmed by the high number of lymphocytes infiltrates noted in the different organs. We conclude that CE at the dose up of 1 g/kg produced toxic effect in mice that induced an immune inflammatory reaction. Abbreviations Used: CE: Caralluma europaea, ALT: Alanine aminotransferase, AST: Aspartate aminotransferase, RRBCs: Rat red blood cells, DTH: Delayed-type hypersensitivity response, PBS: Phosphate buffer solution.

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Protective effect of Caralluma fimbriata against high-fat diet induced testicular oxidative stress in rats

Cited by 31 publications

References 49 publications

Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Spirulina Platensis Affects Factors Involved in Spermatogenesis and Increases Ghrelin Receptors in Testis Tissue of Rats Fed a High-Fat Diet

Toxicological evaluation of the aqueous extract of Caralluma europaea and its immunomodulatory and inflammatory activities

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