Protective effect of citronellol in rhabdomyolysis-induced acute kidney injury in mice

Mahmood, Yasameen Shakir; ,; Kathem, Sarmed Hashim; ,

doi:10.25122/jml-2023-0102

Cited by 3 publications

(1 citation statement)

References 28 publications

(38 reference statements)

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“…The proximal part of the jejunum was extracted and homogenized [ 19 ]. Gene expressions (mRNA) of TLR4, NF-κB, IRF3, AP-1, iNOS, IL-1β, TNF-α, COX-2, and Nrf2 were measured using qRT-PCR, with GAPDH used as a housekeeping gene [ 20 , 21 , 22 ]. Using the TransZol Up Plus RNA Kit (TransGen, biotech Beijing 100192, China), total RNA was isolated from the intestinal homogenate using TRIzol; thereafter, complementary DNA (cDNA) synthesis was carried out using the EasyScript ® one-step gDNA removal and cDNA synthesis (TransGen, biotech).…”

Section: Methodsmentioning

confidence: 99%

Limonene Exerts Anti-Inflammatory Effect on LPS-Induced Jejunal Injury in Mice by Inhibiting NF-κB/AP-1 Pathway

Kathem,

Nasrawi,

Mutlag

et al. 2024

Biomolecules

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The human gastrointestinal system is a complex ecosystem crucial for well-being. During sepsis-induced gut injury, the integrity of the intestinal barrier can be compromised. Lipopolysaccharide (LPS), an endotoxin from Gram-negative bacteria, disrupts the intestinal barrier, contributing to inflammation and various dysfunctions. The current study explores the protective effects of limonene, a natural compound with diverse biological properties, against LPS-induced jejunal injury in mice. Oral administration of limonene at dosages of 100 and 200 mg/kg was used in the LPS mouse model. The Murine Sepsis Score (MSS) was utilized to evaluate the severity of sepsis, while serum levels of urea and creatinine served as indicators of renal function. Our results indicated that LPS injection induced renal function deterioration, evidenced by elevated serum urea and creatinine levels compared to control mice. However, pretreatment with limonene at doses of 100 and 200 mg/kg mitigated this decline in renal function, evidenced from the reduced levels of serum urea and creatinine. Limonene demonstrated anti-inflammatory effects by reducing pro-inflammatory cytokines (TNF-α, IL-1β, COX-2), suppressing the TLR4/NF-κB/AP-1 but not IRF3 signaling pathways, and modulating oxidative stress through Nrf2 activation. The results suggest that limonene holds promise as a potential therapeutic agent for mitigating intestinal inflammation and preserving gastrointestinal health.

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Section: Methodsmentioning

confidence: 99%

Limonene Exerts Anti-Inflammatory Effect on LPS-Induced Jejunal Injury in Mice by Inhibiting NF-κB/AP-1 Pathway

Kathem,

Nasrawi,

Mutlag

et al. 2024

Biomolecules

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Chenopodium botrys Extract Affects Acute Kidney Injury Caused by Rhabdomyolysis in Rats Through TNF/NF‐κB Signaling Pathway

Momeni‐Moghaddam,

Abbasnezhad,

Ebadi

et al. 2024

Food Science & Nutrition

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Due to the anti‐inflammatory and antioxidant properties of the Chenopodium botrys and the pathological mechanisms of rhabdomyolysis in the kidney, this plant can be used to improve the symptoms of this disease. Then, in this study, we investigated the effects of this herb in improving kidney injury by rhabdomyolysis. Animals were divided into five groups: control, glycerol (received it for rhabdomyolysis induction), extract (received 12 mg/kg C. botrys extract), and treatment groups with dexamethasone (0.03 mg/kg) and extract (12 mg/kg). The extract was analyzed using HNMR. After a week, blood and urine samples were taken to measure protein, urea, and creatinine. Then, the animals were sacrificed, and the kidney tissue was removed to examine the antioxidant, TNF‐α, and histopathological evaluations. Also, NF‐κB gene expression was investigated. The serum creatinine, TNF‐α, and NF‐κB ratio significantly increased and antioxidant capacity decreased in the glycerol group compared with the control. Pathological evaluation also showed severe renal damage based on the related criteria. In the treatment groups with dexamethasone and especially extract, the considered parameters attenuated relatively compared with the glycerol group. Kidney damage and functional impairment associated with rhabdomyolysis, as well as the inflammatory response caused by increased NF‐κB and the proinflammatory cytokine TNF‐α, may be alleviated by C. botrys. Consequently, C. botrys could represent a potential therapeutic approach for patients with rhabdomyolysis‐induced acute kidney injury.

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Citronellol protects renal function by exerting anti-inflammatory and antiapoptotic effects against acute kidney injury induced by folic acid in mice

Jamal,

Kathem

2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Protective effect of citronellol in rhabdomyolysis-induced acute kidney injury in mice

Cited by 3 publications

References 28 publications

Limonene Exerts Anti-Inflammatory Effect on LPS-Induced Jejunal Injury in Mice by Inhibiting NF-κB/AP-1 Pathway

Limonene Exerts Anti-Inflammatory Effect on LPS-Induced Jejunal Injury in Mice by Inhibiting NF-κB/AP-1 Pathway

Chenopodium botrys Extract Affects Acute Kidney Injury Caused by Rhabdomyolysis in Rats Through TNF/NF‐κB Signaling Pathway

Citronellol protects renal function by exerting anti-inflammatory and antiapoptotic effects against acute kidney injury induced by folic acid in mice

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