Protective Effect of Clostridium butyricum on Escherichia coli-Induced Endometritis in Mice via Ameliorating Endometrial Barrier and Inhibiting Inflammatory Response

Wang, Kexin; Wang, Ke; Wang, Junrong; Yang, Fan; Ye, Cong

doi:10.1128/spectrum.03286-22

Cited by 12 publications

(4 citation statements)

References 65 publications

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“…Conversely, IL-10 serves as an anti-inflammatory regulator that not only inhibits the overexpression of proinflammatory factors, but also regulates intestinal homeostasis during immune defense processes ( Gao et al, 2012 ; Zigmond et al, 2014 ; Xu S. Q. et al, 2020 ). Generally speaking, CB primarily regulates the secretion of inflammatory cytokines through Toll-like receptor (TLR) pathways, such as TLR4/NF-κB pathway and TLR2/MyD88-independent pathway ( Hayashi et al, 2013 ; Wang et al, 2022 ).Then, we further uncovered the potential molecular pathways that contribute to anti-inflammatory properties of CBX 2021 through transcriptomics analysis. Interestingly, GSEA intuitively showed that CBX 2021 enhanced GPCR activity and its signaling pathway, while downregulating the Notch signaling pathway and the production of proinflammatory factors (IL-6 and TNF-α).…”

Section: Discussionmentioning

confidence: 99%

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Liu,

Qiu,

Yang

et al. 2024

Front. Microbiol.

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IntroductionAs a symbiotic probiotic for the host, Clostridium butyricum (CB) has the potential to strengthen the body’s immune system and improve intestinal health. However, the probiotic mechanism of CB is not completely understood. The Clostridium butyricum CBX 2021 strain isolated by our team from a health pig independently exhibits strong butyric acid production ability and stress resistance. Therefore, this study comprehensively investigated the efficacy of CBX 2021 in pigs and its mechanism of improving pig health.MethodsIn this study, we systematically revealed the probiotic effect and potential mechanism of the strain by using various methods such as microbiome, metabolites and transcriptome through animal experiments in vivo and cell experiments in vitro.ResultsOur in vivo study showed that CBX 2021 improved growth indicators such as daily weight gain in weaned piglets and also reduced diarrhea rates. Meanwhile, CBX 2021 significantly increased immunoglobulin levels in piglets, reduced contents of inflammatory factors and improved the intestinal barrier. Subsequently, 16S rRNA sequencing showed that CBX 2021 treatment implanted more butyric acid-producing bacteria (such as Faecalibacterium) in piglets and reduced the number of potentially pathogenic bacteria (like Rikenellaceae RC9_gut_group). With significant changes in the microbial community, CBX 2021 improved tryptophan metabolism and several alkaloids synthesis in piglets. Further in vitro experiments showed that CBX 2021 adhesion directly promoted the proliferation of a porcine intestinal epithelial cell line (IPEC-J2). Moreover, transcriptome analysis revealed that bacterial adhesion increased the expression of intracellular G protein-coupled receptors, inhibited the Notch signaling pathway, and led to a decrease in intracellular pro-inflammatory molecules.DiscussionThese results suggest that CBX 2021 may accelerate piglet growth by optimizing the intestinal microbiota, improving metabolic function and enhancing intestinal health.

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Section: Discussionmentioning

confidence: 99%

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Liu,

Qiu,

Yang

et al. 2024

Front. Microbiol.

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“…Similarly, Lactococcus belongs to the Streptococcus family due to its production of bacteriocins and organic acids and has a number of beneficial functions [ 43 ]. An increase in the abundance of Clostridium populations has been related to a higher production of butyric acid [ 44 ], which can be absorbed and used as an energy source by colonic epithelial cells [ 45 ] and is able to modulate the immune system [ 46 ]. Parabacteroides is related to intestinal integrity disruption, with a higher abundance of Parabacteroides reflecting good intestinal barrier function [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens

Zhu

Bai

Xia

et al. 2022

Animals

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Restrictions on antibiotics are driving the search for alternative feed additives to promote gastrointestinal health and development in broiler chicken production. Proteins including antimicrobial peptides can potentially be applied as alternatives to antibiotics and are one of the most promising alternatives. We investigated whether the addition of MPX to the diet affects the production performance, immune function and the intestinal flora of the caecal contents of broiler chickens. One hundred one-day-old chickens were randomly divided into two groups: control (basal diet) and MPX (20 mg/kg) added to the basal diet. The results indicated that dietary supplementation with MPX improved the performance and immune organ index, decreased the feed conversion ratio, increased the villus length, maintained the normal intestinal morphology and reduced the IL-6 and LITNF mRNA expression levels of inflammation-related genes. In addition, MPX increased the mRNA expression of the digestive enzymes FABP2 and SLC2A5/GLUT5 and the tight junction proteins ZO-1, Claudin-1, Occludin, JAM-2 and MUC2, maintained the intestinal permeability and regulated the intestinal morphology. Moreover, MPX increased the CAT, HMOX1 and SOD1 mRNA expression levels of the antioxidant genes. Furthermore, a 16S rRNA microflora analysis indicated that the abundance of Lactobacillus and Lactococcus in the cecum was increased after addition of MPX at 14 d and 28 d. This study explored the feasibility of using antimicrobial peptides as novel feed additives for broiler chickens and provides a theoretical basis for their application in livestock.

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“…In addition to these common probiotics, there is a need to explore novel probiotics targeting NDs. Clostridium butyricum is a notable probiotic that can ferment carbohydrates into butyric acid and has excellent protective effects against pathogenic microorganisms [ 130 ]. An animal study showed that a 4-week treatment with Clostridium butyricum (5 × 10 8 CFU/day) effectively protected against cognitive impairment and amyloid pathology by reducing microglia-mediated neuroinflammation in an AD (APP/PS1) mouse model [ 131 ].…”

Section: Potential Clinical Approaches Targeting the Microbiome In Ndsmentioning

confidence: 99%

Therapeutics for neurodegenerative diseases by targeting the gut microbiome: from bench to bedside

Ma,

Li,

et al. 2024

Transl Neurodegener

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The aetiologies and origins of neurodegenerative diseases, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS) and Huntington’s disease (HD), are complex and multifaceted. A growing body of evidence suggests that the gut microbiome plays crucial roles in the development and progression of neurodegenerative diseases. Clinicians have come to realize that therapeutics targeting the gut microbiome have the potential to halt the progression of neurodegenerative diseases. This narrative review examines the alterations in the gut microbiome in AD, PD, ALS and HD, highlighting the close relationship between the gut microbiome and the brain in neurodegenerative diseases. Processes that mediate the gut microbiome–brain communication in neurodegenerative diseases, including the immunological, vagus nerve and circulatory pathways, are evaluated. Furthermore, we summarize potential therapeutics for neurodegenerative diseases that modify the gut microbiome and its metabolites, including diets, probiotics and prebiotics, microbial metabolites, antibacterials and faecal microbiome transplantation. Finally, current challenges and future directions are discussed.

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Protective Effect of Clostridium butyricum on Escherichia coli-Induced Endometritis in Mice via Ameliorating Endometrial Barrier and Inhibiting Inflammatory Response

Cited by 12 publications

References 65 publications

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens

Therapeutics for neurodegenerative diseases by targeting the gut microbiome: from bench to bedside

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