Protective Effect of Combination of Sulforaphane and Riluzole on Glutamate-Mediated Excitotoxicity

An overview on neuroprotective effects of isothiocyanates for the treatment of neurodegenerative diseases

“…In 2010, Chang et al investigated whether SFN can act together with riluzole, the only medicine already approved in ALS treatment, in protecting motor neurons against glutamate induced excitotoxicity in organotypic spinal cord cultures [64]. In particular, the efficacies of the two treatments alone or in combination in inhibiting progression and severity of disease were tested.…”

“…The results showed that the combination of SFN with riluzole is more effective than each drug used alone, as the combination not only stimulates the expression of Nrf2 pathway, but also reduces the extracellular accumulation of glutamate. Furthermore, the combination of the two treatments exerts significant and similar neuroprotection, as measured by the number of motor neuron, medium malondialdehyde (MDA) level and LDH level [64]. Therefore, SFN could be used in association with conventional therapy in ALS management.…”

“…[ [62][63][64] GMG-ITC Counteracts oxidative stress by modulating iNOS, MMP-9, Nrf2 and PARP expression, as well as apoptotic pathway (cleaved-caspase 3 and TUNEL assay).…”

A study of the potential neuroprotective effect of riluzole on locomotor networks of the neonatal rat spinal cord in vitro damaged by excitotoxicity

“…As previous studies had shown the positive effect of pre or co-application of riluzole on chemically evoked neuronal damage (Chang et al, 2010;Verhave et al, 2012), we tested, on a further group of spinal cords (n = 3), co-application of kainate (0.05 mM) and riluzole (5 lM) followed by 3 h application of riluzole alone. Despite this protocol, fictive locomotion was never observed 24 h later.…”

“…A large number of studies that have been performed to clarify if riluzole could combat various types of neurodegeneration, have produced mixed results depending on the models used in vivo (Verhave et al, 2012) or in vitro (Rammes et al, 2008;Chang et al, 2010;Mazzone and Nistri, 2011).…”