Protective effect of crocin on diazinon induced vascular toxicity in subchronic exposure in rat aorta<i>ex-vivo</i>

Razavi, Bibi Marjan; Hosseinzadeh, Hossein; Abnous, Khalil; Imenshahidi, Mohsen

doi:10.3109/01480545.2013.866139

Cited by 25 publications

(17 citation statements)

References 21 publications

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“…Moreover, the cardiovascular protective effects of crocin are considered to be closely associated with its antioxidant activity [1, 5, 11, 32, 33]. Additional research has also shown that biocompounds from saffron, including crocin, exert a hypotensive effect in normotensive and hypertensive states [1, 7].…”

Section: Discussionmentioning

confidence: 99%

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Crocin Improves the Endothelial Function Regulated by Kca3.1 Through ERK and Akt Signaling Pathways

Yang

Liu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Based on the protective effect of crocin against cardiovascular diseases, we hypothesize that crocin could improve endothelial function through activating the eNOS(endothelial nitric oxide synthase) /NO pathway and/or the intermediate-conductance Ca²⁺-activated K⁺ channels (KCa3.1). Methods: In this study, rat aortic rings were used to assess the regulatory effect of crocin on vascular tone and nitric oxide, prostacyclin, and KCa3.1, all endothelial vasodilators, were analyzed for effects by crocin. The expression profiles of p-eNOS, total-eNOS, p-ERK, total-ERK, p-Akt, total-Akt, KCa3.1, CD31, thrombomodulin, ICAM-1 and VCAM-1 were tested by western blotting. KCa3.1 was also analyzed by qPCR and immunofluorescence staining. Fluorescence and confocal microscopy were used to determine NO generation and intracellular Ca²⁺. Both EdU and MTT assays were used to evaluate cell viability. Cellular migration was assessed using transwell assay. Results: Crocin relaxed pre-contracted artery rings through either NO or KCa3.1, but not PGI, in an endothelium-dependent manner. Furthermore, crocin increased p-eNOS, total-eNOS expression and NO production as well as intracellular Ca²⁺ in both HUVECs and HUAECs (Human Umbilical Artery Endothelial cells). Crocin also stimulated the expression of CD31, thrombomodulin and vascular cell adhesion molecule 1 (VCAM-1), as well as increased cellular proliferation and migration in vitro. Interestingly, we determined for the first time that by blocking or silencing KCa3.1 there was inhibition of crocin induced upregulation of p-eNOS and total-eNOS. Correspondingly, the KCa3.1 inhibitor TRAM-34 also reduced the expression of CD31, thrombomodulin and VCAM-1, as well as diminished intracellular Ca²⁺, cellular proliferation and migration. Finally, crocin stimulated the expression of p-ERK, total-ERK, p-Akt and total-Akt, however suppression of MEK and Akt inhibited this expression profile in endothelial cells. Conclusion: In the present study, these data strongly support the hypothesis that crocin could improve endothelial function through stimulation of the eNOS/NO pathway and other endothelial markers. This functional improvement is regulated by KCa3.1 via the MEK/ERK and PI3K/Akt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

“…Crocin is one of the major pharmacologically bioactive constituents in saffron [1, 5, 32], and possesses protective effects against cardiovascular diseases [1, 3, 5-7, 33]. Moreover, the cardiovascular protective effects of crocin are considered to be closely associated with its antioxidant activity [1, 5, 11, 32, 33].…”

Section: Discussionmentioning

confidence: 99%

Crocin Improves the Endothelial Function Regulated by Kca3.1 Through ERK and Akt Signaling Pathways

Yang

Liu

et al. 2018

Cell Physiol Biochem

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“…It was also indicated that down regulation of eNOS expression as a result of oxidized low density lipoprotein (LDL) and increased NO production in bovine aortic endothelial cells (BAECs) were inhibited by crocetin (34). Result of another study showed that crocin could improve vascular toxic effects of diazinon by restoring altered contractile responses to PE and KCl and impaired relaxant responses to acetylcholine and sodium nitroprusside in rat aorta (35). Moreover, crocin restored the effects of sub chronic diazinon administration on systolic blood pressure in rats (36).…”

Section: Discussionmentioning

confidence: 99%

Crocin-Induced Endothelium-Dependent Relaxation in Isolated Rat Aorta

Razavi

Seyfabad

Hosseinzadeh

et al. 2016

Jundishapur J Nat Pharm Prod

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“…43 In a similar study based on rat models, it was concluded that crocin improves toxic effects of diazinon through decreasing lipid peroxidation and restoring altered contractile and relaxant responses in aorta. 44 The cardio-protective effect of saffron active constituents including crocin has been confirmed through regulation of oxidative stress. 45 The finding concluded that Crocus sativus perfused during electrolysis might trap radical oxygen species and significantly improve myocardial function.…”

Section: Nephrotoxicity Effectmentioning

confidence: 99%

Saffron (Crocus sativus) and its Active Ingredients: Role in the Prevention and Treatment of Disease

Rahmani¹,

Khan²,

Aldebasi³

2017

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Introduction:Crocus sativus is a member of iridaceae family and its use in health management is in practice since ancient time. Additionally, research based on clinical trials and animal models have advocates its role in disease cure without any severe adverse complications. The aim of this study is to summarize the study of saffron and its ingredients based on in vivo and in vitro and role in disease cure and prevention. Materials: This study was designed through the search engines such as Pub Med, Scopus and Google Scholar. The keywords used for the search were mainly focused on Saffron with health benefit and its therapeutics role in different diseases. Result: Saffron stigma is mixture of various components and recent studies have proven that saffron and its active ingredients play a key role in disease management. Moreover, clinical trials based study on the use of saffron and its individual components have confirmed the health promising effects. Conclusion: Although Saffron has potential role in the disease cure and prevention via modulation of anti-oxidant, anti-inflammatory, anti-tumour, anti-microbial and anti-diabetic activity. Furthermore, advanced research is needed to elaborate the role of saffron in health management and its mechanism of action in the modulation of biological activities.

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Protective effect of crocin on diazinon induced vascular toxicity in subchronic exposure in rat aortaex-vivo

Abstract: DZN induced vascular toxicity which may be due to oxidative stress and not to a cholinergic mechanism. Crocin improved toxic effects of DZN via reducing lipid peroxidation and restoring altered contractile and relaxant responses in rat aorta.

Cited by 25 publications

References 21 publications

Crocin Improves the Endothelial Function Regulated by Kca3.1 Through ERK and Akt Signaling Pathways

Crocin Improves the Endothelial Function Regulated by Kca3.1 Through ERK and Akt Signaling Pathways

Crocin-Induced Endothelium-Dependent Relaxation in Isolated Rat Aorta

Saffron (Crocus sativus) and its Active Ingredients: Role in the Prevention and Treatment of Disease

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