Protective effect of cultured bear bile powder against dimethylnitrosamine-induced hepatic fibrosis in rats

Zheng, Min; Li, Yuanyuan; Wang, Guofeng; Jin, Jingyi; Wang, Yahang; Wang, Tianming; Yang, Li; Liu, Shaoyong; Wu, Jiasheng; Wang, Zhengtao; Ma, Yueming

doi:10.1016/j.biopha.2019.108701

Cited by 17 publications

(15 citation statements)

References 38 publications

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“…More commonly, MS-based methods have shown elevations in specific serum bile acids in human liver cirrhosis [128,132,135], together with fluctuations in a broad range of urinary steroids [141]. Another common finding were alterations in serum phospholipids in both human liver cirrhosis [135,139,140] and in animal models [169,170,172]. In both the human and animal model investigations, perturbations in metabolic intermediates, akin to those revealed by NMR, have also been described.…”

Section: Uplc-qtofmsmentioning

confidence: 99%

“…Moreover, expression of four PPARα-regulated genes involved in fatty acid β-oxidation (Cpt1b, Cpt2, Mcad, and Hadha) was decreased by DMN treatment but restored by CBBP, suggesting that CBBP may improve fatty acid β-oxidation. By inducing PPARγ, CBBP decreased the downstream expression of the inflammatory cytokine IL-6, while also inhibiting activation of hepatic stellate cells, thereby ameliorating fibrogenesis [172]. Further details of the aforementioned studies appear in Table 3.…”

Section: Fibrosis and Cirrhosismentioning

confidence: 99%

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Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Beyoğlu

Idle

2020

Metabolites

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In recent years, there has been a plethora of attempts to discover biomarkers that are more reliable than α-fetoprotein for the early prediction and prognosis of hepatocellular carcinoma (HCC). Efforts have involved such fields as genomics, transcriptomics, epigenetics, microRNA, exosomes, proteomics, glycoproteomics, and metabolomics. HCC arises against a background of inflammation, steatosis, and cirrhosis, due mainly to hepatic insults caused by alcohol abuse, hepatitis B and C virus infection, adiposity, and diabetes. Metabolomics offers an opportunity, without recourse to liver biopsy, to discover biomarkers for premalignant liver disease, thereby alerting the potential of impending HCC. We have reviewed metabolomic studies in alcoholic liver disease (ALD), cholestasis, fibrosis, cirrhosis, nonalcoholic fatty liver (NAFL), and nonalcoholic steatohepatitis (NASH). Specificity was our major criterion in proposing clinical evaluation of indole-3-lactic acid, phenyllactic acid, N-lauroylglycine, decatrienoate, N-acetyltaurine for ALD, urinary sulfated bile acids for cholestasis, cervonoyl ethanolamide for fibrosis, 16α-hydroxyestrone for cirrhosis, and the pattern of acyl carnitines for NAFL and NASH. These examples derive from a large body of published metabolomic observations in various liver diseases in adults, adolescents, and children, together with animal models. Many other options have been tabulated. Metabolomic biomarkers for premalignant liver disease may help reduce the incidence of HCC.

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Section: Uplc-qtofmsmentioning

confidence: 99%

Section: Fibrosis and Cirrhosismentioning

confidence: 99%

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Beyoğlu

Idle

2020

Metabolites

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“…4675) and P-value (p=0.0214 or p=0.0213), the results indicated serum levels of IL-1b and TNF-a had positive linear correlations with PGE 2 levels ( Figures 6E, F). The Pearson's correlation method was descripted as previously reported (Yan et al, 2017;Zheng et al, 2019). Additionally, the mRNA and protein expression of IL-1b and IL-6 in rat colon tissue was also investigated by real-time PCR and immunohistochemistry.…”

Section: Effect Of Bzyqd On Inflammatory Response In Rats With Loperamentioning

confidence: 99%

“…Metabolomics represents a powerful discipline concerned with the comprehensive analysis of small molecules to discover biomarkers in biological systems (Schrimpe-Rutledge et al, 2016;Zhang et al, 2019). This method provides holistic insights into changes in the metabolic pathways during disease or drug treatment (Wu et al, 2017;Zheng et al, 2019). Therefore, metabolomics study is in perfect accordance with the holistic approach of TCM, and can provide clues to mechanism of action of TCM (Wu et al, 2017;Zheng et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…This method provides holistic insights into changes in the metabolic pathways during disease or drug treatment (Wu et al, 2017;Zheng et al, 2019). Therefore, metabolomics study is in perfect accordance with the holistic approach of TCM, and can provide clues to mechanism of action of TCM (Wu et al, 2017;Zheng et al, 2019). In the current study, the effect of BZYQD on loperamide-induced constipation in a rat model was investigated.…”

Section: Introductionmentioning

confidence: 99%

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Buzhongyiqi Decoction Protects Against Loperamide-Induced Constipation by Regulating the Arachidonic Acid Pathway in Rats

Zhao

Chen

et al. 2020

Front. Pharmacol.

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Constipation is a common gastrointestinal disorder without effective treatment approach. Buzhongyiqi decoction (BZYQD) is a classical formula that has been commonly used for gastrointestinal disorders for nearly 1,000 years. In this study, we aimed to investigate the protective effect of BZYQD against loperamide-induced constipation and its potential mechanism. Rats with loperamide-induced constipation were orally administered BZYQD. BZYQD treatment obviously increased the small intestinal transit rate and alleviated colon tissue pathological damage. Subsequently, serum metabolomics study was performed to identify the metabolites affected by BZYQD. Metabolomics identified that the levels of 17 serum metabolites, including prostaglandin E 2 (PGE 2), arachidonic acid (AA), and inositol, were significantly changed in BZYQD-treated group compared with those in the loperamide-induced group. Pathway analysis revealed that those metabolites were mainly associated with arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, ascorbate and aldarate metabolism, inositol phosphate metabolism. Additionally, BZYQD treatment down-regulated the cyclooxygenase-2 expression and decrease production of the proinflammatory mediator PGE 2. Further study revealed that BZYQD administration decreased serum levels of the inflammatory factors IL-1b and TNF-a, inhibited phosphorylation of the nuclear transcription factor NF-kB, and down-regulated expression of the inflammatory factors IL-1b and IL-6 in the constipated rat colon. Moreover, BZYQD treatment also increased serum levels of inositol, motilin and gastrin, and promoted gastrointestinal motility. In conclusion, the present study suggested that BZYQD exerted a protective effect against loperamide-induced constipation, which may be associated with its role in regulation of multiple metabolic pathways.

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A validated surrogate analyte UPLC–MS/MS assay for quantitation of TUDCA, TCDCA, UDCA and CDCA in rat plasma: Application in a pharmacokinetic study of cultured bear bile powder

Zan

Xin-hua

Zhao

et al. 2020

Biomedical Chromatography

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Bear bile is a valuable medicinal material used in traditional Chinese medicine for over 2000 years. However, developing a substitute has become necessary because of protection measures for this endangered species. The ingredients of in vitro cultured bear bile powder (CBBP) include tauroursodeoxycholic acid (TUDCA), taurochenodeoxycholic acid (TCDCA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA, and it has pharmacological properties that are similar to those of natural bear bile powder (NBBP). In this study, the pharmacokinetic parameters of both CBBP and NBBP were measured in rats with a new surrogate analyte LC–MS method using stable isotopes as surrogate analytes (D4‐TUDCA, D4‐TCDCA, D4‐UDCA and D4‐CDCA) with response factors validated in authentic matrix (plasma) for simultaneously monitoring the authentic analytes (TUDCA, TCDCA, UDCA and CDCA). The method validation was satisfactory for the linear regression (r, 0.9975–0.9994), precision (RSD intra‐day, 0.72–9.35%; inter‐day, 3.82–9.02%), accuracy (RE, −12.42–5.67%) and matrix effect (95.53–99.80%), along with analyte recovery (95.90–98.82%) and stability (89.48–101.81%) of surrogate analytes, and precision (RSD intra‐day, 1.06– 11.51%; inter‐day, 2.23– 11.38%), accuracy (RE, −7.40–10.76%) and stability (87.37–111.70%) of authentic analytes. We successfully applied this method to evaluate the pharmacokinetics of CBBP and NBBP in rats, which revealed the critical in vivo properties of both bear bile preparations.

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Protective effect of cultured bear bile powder against dimethylnitrosamine-induced hepatic fibrosis in rats

Cited by 17 publications

References 38 publications

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Buzhongyiqi Decoction Protects Against Loperamide-Induced Constipation by Regulating the Arachidonic Acid Pathway in Rats

A validated surrogate analyte UPLC–MS/MS assay for quantitation of TUDCA, TCDCA, UDCA and CDCA in rat plasma: Application in a pharmacokinetic study of cultured bear bile powder

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