Protective Effect of Curcumin Against Acute Ultraviolet B Irradiation‐induced Photo‐damage

Li, Huaping; Gao, Aili; Jiang, Na; Liu, Qing; Liang, Bihua; Li, Runxiang; Zhang, Erting; Li, Zhenjie; Zhu, Huilan

doi:10.1111/php.12628

Cited by 58 publications

(44 citation statements)

References 29 publications

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“…In chemotherapy, curcumin has positive effects and is a chemosensitizer and also reduces toxic and adverse effects of chemotherapeutic drugs (Goel & Aggarwal, ; Rezaee, Momtazi, Monemi, & Sahebkar, ). In addition, curcumin has been shown radioprotective effects on normal cells (Goel & Aggarwal, ; Inano & Onoda, ) and protective effects against damage caused by ultraviolet B (Li et al, ). Turmeric and curcumin have beneficial effects on the health of the skin (Vaughn, Branum, & Sivamani, ).…”

Section: Introductionmentioning

confidence: 99%

Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review

Soleimani

Sahebkar

Hosseinzadeh

2018

Phytotherapy Research

443

274

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Curcumin is the major constituent of turmeric (Curcuma longa). Turmeric has been widely used as a spice in foods and for therapeutic applications such as anti-inflammatory, antihyperlipidemic, and antimicrobial activities. Turmeric and curcumin are nonmutagenic and nongenotoxic. Oral use of turmeric and curcumin did not have reproductive toxicity in animals at certain doses. Studies on human did not show toxic effects, and curcumin was safe at the dose of 6 g/day orally for 4-7 weeks. However, some adverse effects such as gastrointestinal upsets may occur. Moreover, oral bioavailable formulations of curcumin were safe for human at the dose of 500 mg two times in a day for 30 days, but there are still few trials and more studies are needed specially on nanoformulations and it should be discussed in a separate article. In addition, curcumin is known as a generally recognized as safe substance. This review discusses the safety and toxicity of turmeric and curcumin in medicine. Turmeric and curcumin are nontoxic for human especially in oral administration. Turmeric and curcumin are also safe in animals. They are nonmutagenic and are safe in pregnancy in animals but more studies in human are needed.

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Section: Introductionmentioning

confidence: 99%

Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review

Soleimani

Sahebkar

Hosseinzadeh

2018

Phytotherapy Research

443

274

View full text Add to dashboard Cite

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“…Extrinsic aging occurs due to environmental aggressors, including ultraviolet (UV) irradiation, smoke, and common pollutants [2], whereas exposure to ultraviolet (UV) radiation (particularly UVB) is the leading factor in extrinsic aging, which is also known as photoaging [3]. Dermal fibroblasts, which are the predominant cell type in the dermis, are responsible for regulating the extracellular matrices, interstitial fluid volume and pressure, collagen production, and wound healing [4].…”

Section: Introductionmentioning

confidence: 99%

Rapamycin Protects Skin Fibroblasts from Ultraviolet B-Induced Photoaging by Suppressing the Production of Reactive Oxygen Species

Qin

Ren

Jia

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, thereby inducing skin photoaging. Murine dermal fibroblasts (MDFs) were subjected to a series of 4 sub-cytotoxic UVB doses (120 mJ/cm²), resulting in changes in cell shape, DNA damage, cell cycle arrest, extracellular matrix variations, reactive oxygen species (ROS) generation, and alterations in major intracellular antioxidant and cellular autophagy levels. Rapamycin (RAPA) is a new macrolide immunosuppressive agent that is primarily used in oncology, cardiology, and transplantation medicine and has been found to extend the lifespan of genetically heterogeneous mice. Several studies have shown that RAPA may have anti-aging effects in cells and organisms. Thus, in this study, we explored the effects and mechanisms of RAPA against the photoaging process using a well-established cellular photoaging model. Methods: We developed a stress-induced premature senescence (SIPS) model through repeated exposure of MDFs to ultraviolet B (UVB) irradiation. The cells were cultured in the absence or presence of RAPA for 48 h. Senescent phenotypes were assessed by examining cell viability, cell morphology, senescence-associated β-galactosidase (SA-β-gal) expression, cell cycle progression, intracellular ROS production, matrix metalloproteinase (MMP) synthesis and degradation, extracellular matrix (ECM) component protein expression, alterations in major intracellular antioxidant levels, and the cellular autophagy level. Results: Compared with the UVB group, pretreatment with RAPA (5 µM) significantly decreased the staining intensity and percentage of SA-β-gal-positive cells and preserved the elongated cell shape. Moreover, cells pretreated with RAPA showed inhibition of the reduction in the type I collagen content by blocking the UVB-induced upregulation of MMP expression. RAPA also decreased photoaging cell cycle arrest and downregulated p53 and p21 expression. RAPA application significantly attenuated irradiation-induced ROS release by modulating intracellular antioxidants and increasing the autophagy level. Conclusions: Our study demonstrated that RAPA elicited oxidative damage in vitro by reducing ROS accumulation in photoaged fibroblasts. The anti-aging effect can be attributed to the maintenance of normal antioxidant and cellular autophagy levels. However, determination of the definitive mechanism requires further study.

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“…Measurement of intracellular production of ROS. Intracellular ROS levels were determined by the oxidative conversion of cell-permeable 2',7'-dichlorodihydro-fluorescein diacetate (DCFH-DA) to fluorescent DCF, as previously described (26). cells (3.5x10 4 /ml) were seeded in a 96-well plate and pretreated with dMEM containing ASB for 24 h. Then, the cells were irradiated with UV (300 µW/cm 2 sec x 300 sec).…”

Section: Methodsmentioning

confidence: 99%

Andrographolide sodium bisulfate attenuates UV‑induced photo‑damage by activating the keap1/Nrf2 pathway and downregulating the NF‑κB pathway in HaCaT keratinocytes

Wang¹,

Zhong

Lin

et al. 2019

Int J Mol Med

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Oxidative and inflammatory damage has been suggested to play important roles in the pathogenesis of skin photoaging. Andrographolide sodium bisulfate (ASB) is a soluble derivative of andrographolide and has known antioxidant and anti-inflammatory properties. In the present study, cellular experiments were designed to investigate the molecular mechanisms underlying the effect of ASB in relieving ultraviolet (UV)-induced photo-damage. Following ASB pretreatment and UV irradiation, the apoptosis and necrosis of HacaT cells were investigated by Hoechst 33342/propidium iodide staining. Reactive oxygen species (ROS) production was investigated using a dcFH-dA fluorescence probe. Furthermore, the protein expression levels of p65, NF-κB inhibitor-α, nuclear factor E2-related factor 2 (Nrf2) and kelch-like EcH-associated protein 1 (keap1) were measured via western blotting and immunofluorescence analyses. Furthermore, NF-κB-mediated cytokines were assessed by ELISA, and Nrf2-mediated genes were detected by reverse transcription-quantitative PcR. Pretreatment with ASB markedly increased cell viability, decreased cell apoptosis and decreased UV-induced excess ROS levels. In addition, ASB activated the production of Nrf2 and increased the mRNA expression levels of glutamate-cysteine ligase catalytic subunit and NAd(P)H quinone oxidoreductase 1, while ASB downregulated the protein expression of p65 and decreased the production of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α. These results suggested that ASB attenuates UV-induced photo-damage by activating the keap1/Nrf2 pathway and downregulating the NF-κB pathway in HacaT keratinocytes.

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Protective Effect of Curcumin Against Acute Ultraviolet B Irradiation‐induced Photo‐damage

Cited by 58 publications

References 29 publications

Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review

Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review

Rapamycin Protects Skin Fibroblasts from Ultraviolet B-Induced Photoaging by Suppressing the Production of Reactive Oxygen Species

Andrographolide sodium bisulfate attenuates UV‑induced photo‑damage by activating the keap1/Nrf2 pathway and downregulating the NF‑κB pathway in HaCaT keratinocytes

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