Protective effect of cystathionine on acute gastric mucosal injury induced by ischemia-reperfusion in rats

Wada, Kouichirou; Kamisaki, Yoshínori; Kitano, Masayuki; Nakamoto, Kentaro; Itoh, Tadao

doi:10.1016/0014-2999(95)00558-7

Cited by 53 publications

(49 citation statements)

References 15 publications

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“…Sulfur-containing reducing compounds such as glutathione and cysteine have been reported to protect the lens from oxidative damage (18,28,29). Interestingly, cystathionine, a transsulfuration intermediate, has been reported to be a superoxide radical quencher (42,43), and under hyperoxia its levels have been shown to be increased significantly in lung tissue (44). In this study, our analysis of metabolites revealed that when compared with cow and rat lenses, monkey lenses contained 10 -20-fold higher cystathionine.…”

Section: Table IImentioning

confidence: 55%

Betaine-homocysteine Methyltransferase Is a Developmentally Regulated Enzyme Crystallin in Rhesus Monkey Lens

Rao¹,

Garrow²,

John³

et al. 1998

Journal of Biological Chemistry

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We describe herein the characterization of a major 45-kDa protein from the soluble ␤H-crystallin fraction of rhesus monkey (Macaca mulatta) lens. Based on partial peptide sequence, immunoreactivity, and enzymatic activity, this protein has been identified as betaine-homocysteine S-methyltransferase (BHMT: EC 2.1.1.5), an enzyme that catalyzes the methylation of homocysteine using either betaine or thetins as methyl donors. This protein was found to be expressed abundantly in the nuclear region of the monkey lens, reaching ϳ10% of the total nuclear protein, but was barely detectable in the epithelium and cortex regions of the lens. Because the nucleus represents the early embryonic and fetal stages of lens development, we infer that BHMT expression in the lens of the eye is developmentally regulated. By virtue of its high abundance, BHMT can be considered an enzyme crystallin (-crystallin). This is the first enzyme crystallin to be found in primate lenses.

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Section: Table IImentioning

confidence: 55%

Betaine-homocysteine Methyltransferase Is a Developmentally Regulated Enzyme Crystallin in Rhesus Monkey Lens

Rao¹,

Garrow²,

John³

et al. 1998

Journal of Biological Chemistry

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“…The superoxide radical scavenger superoxide dismutase and the hydroxyl radical scavenger dimethyl sulfoxide attenuate ischemia-reperfusion injury (6,41,42). In this regard, neither irsogladine nor the PDE4 inhibitor rolipram show the superoxide-radical-scavenging effect in a cell-free hypoxanthine-xanthine oxidase superoxide generating system (24).…”

Section: Discussionmentioning

confidence: 99%

Phosphodiesterase Type IV Inhibitors Prevent Ischemia-Reperfusion-Induced Gastric Injury in Rats

et al. 2004

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Abstract. The effects of selective inhibitors of phosphodiesterase type IV (PDE4) on ischemiareperfusion-induced gastric injuries were investigated in rats. Gastric ischemia was induced by applying a small clamp to the celiac artery, and reoxygenation was performed by removal of the clamp. Ischemia-reperfusion produced gastric hemorrhagic injuries and increased the content of the proinflammatory cytokine tumor necrosis factor-a (TNF-a ) and myeloperoxidase (MPO) activity in gastric mucosa. Rolipram (0.03 -0.3 mg / kg, s.c.) and Ro-20-1724 (0.3 -3 mg/ kg, s.c.) prevented the development of gastric injury in a dose-dependent manner, and it also inhibited the increase in mucosal TNF-a content and MPO activity induced by ischemiareperfusion. The anti-ulcer drug irsogladine (1 -10 mg / kg, p.o.), which is known to possess a PDE4 inhibitory action, also inhibited the gastric injury produced by ischemia-reperfusion, as well as the increase in TNF-a levels and MPO activity. It is concluded that the ability of PDE4 inhibitors to inhibit cytokine TNF-a synthesis and the infiltration of polymorphonuclear leukocytes underlies their gastroprotective effects in ischemia-reperfusion-induced gastric injury. Our experiments suggest that drugs that inhibit PDE4 isoenzyme, such as the anti-ulcer drug irsogladine, may be a useful adjunct therapy for the treatment of the gastric damage that follows ischemia-reperfusion.

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“…The maximal changes in blood pressure and renal blood flow were observed approximately 1 -2 min after administration of each test compound. The mean blood pressure is expressed in mmHg, and the renal blood flow is expressed in mV according to the previous reports in which the same laser Doppler flowmeter was used for measuring renal blood flow (16,17).…”

Section: Experimental Protocolmentioning

confidence: 99%

Adrenomedullin Inhibits the Pressor Effects and Decrease in Renal Blood Flow Induced by Norepinephrine or Angiotensin II in Anesthetized Rats

Minami

Segawa

Uezono

et al. 2001

Japanese Journal of Pharmacology

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ABSTRACT-Adrenomedullin (AM), a hypotensive peptide originally isolated from human pheochromocytoma, has been reported to regulate renal functions. In patients with glomerulonephritis, the serum levels of AM are elevated as well as hypertensive agents norepinephrine (NE) and angiotensin II (AII). The effects of AM on the NE-or AII-induced pressor effects and renal blood flow responses, however, are not well clarified. We examined the effects of AM on blood pressure and renal blood flow induced by NE or AII in anesthetized rats. Arterial blood pressure and renal blood flow were measured using a calibrated pressure transducer and a laser Doppler flowmeter, respectively. Drugs were injected into the tail vein with a syringe. Intravenous administration of AM (1 -3 nmol/ kg) decreased the arterial blood pressure in anesthetized rats in a dose-dependent manner, whereas it did not affect the renal blood flow. NE or AII administration in anesthetized rats caused both increases in blood pressure and decreases in renal blood flow. Simultaneous administration of AM with NE or AII prevented the increasing effects of blood pressure and inhibited the decreases in renal blood flow caused by NE or AII. These findings suggest that AM may have a protective role against the pressor effects and decrease in renal blood flow caused by NE or AII.

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Protective effect of cystathionine on acute gastric mucosal injury induced by ischemia-reperfusion in rats

Cited by 53 publications

References 15 publications

Betaine-homocysteine Methyltransferase Is a Developmentally Regulated Enzyme Crystallin in Rhesus Monkey Lens

Betaine-homocysteine Methyltransferase Is a Developmentally Regulated Enzyme Crystallin in Rhesus Monkey Lens

Phosphodiesterase Type IV Inhibitors Prevent Ischemia-Reperfusion-Induced Gastric Injury in Rats

Adrenomedullin Inhibits the Pressor Effects and Decrease in Renal Blood Flow Induced by Norepinephrine or Angiotensin II in Anesthetized Rats

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