Protective effect of diallyl disulfide on cyclophosphamide-induced testicular toxicity in rats

Abstract

This study investigated the protective effects of diallyl disulfide (DADS) against cyclophosphamide (CP)-induced testicular toxicity in male rats. DADS was gavaged to rats once daily for 3 days at 100 mg/kg/day. One hour after the final DADS treatment, the rats were given a single intraperitoneal dose of 150 mg/kg CP. All rats were killed and necropsied on day 56 after CP treatment. Parameters of testicular toxicity included reproductive organ weight, testicular sperm head count, epididymal sperm motility and morphology, epididymal index, and histopathologic examinations. The CP treatment caused a decrease in body weight, testicular sperm head count, epididymal sperm motility, and epididymal index. The histopathological examination revealed various morphological alterations, characterized by degeneration of spermatogonia/spermatocytes, vacuolization, and decreased number of spermatids/spermatocytes in the testis, and cell debris and mild oligospermia in the ductus epididymis. In contrast, DADS pretreatment effectively attenuated the testicular toxicity caused by CP, including decreased sperm head count, epididymal sperm motility, and epididymal index and increased histopathological alterations in the testis and epididymis. These results indicate that DADS attenuates testicular toxicity induced by CP in rats.

Acrocomia aculeataoil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats

“…The testicular histological findings reaffirm the testicular toxicity of CP, as the major histological changes were found in the CP group, and include vacuolisation and degeneration of germ cells, similar results to those reported by Kim et al. (). Moreover, the testis of the CP group exhibited many seminiferous tubules under three germ layers, corroborating with previous studies (Rezvanfar et al., ).…”

“…Some natural compounds with antioxidant properties have been proven beneficial in the experimental model of reproductive toxicity induced by CP (Abarikwu, Otuechere, Ekor, Monwuba, & Osobu, ; Das et al., ; Kim et al., , ). Although testis and epididymis weights of animals exposed to CP concurrently with the A. aculeata oil did not differ from the CP group, it was noted that the seminal glands and prostate of these animals were apparently protected by the oil or BC.…”

Cyclophosphamide-induced male subfertility in mice: An assessment of the potential benefits of Maca supplement

“…CYP has also been associated with gonadal toxicity in male and female survivors of adult or childhood cancers (Kenney, Laufer, Grant, Grier, & Diller, 2001;Thomson et al, 2002); and patients treated for noncancer related pathologies like rheumatic diseases (Dooley & Nair, 2008;Silva, Bonfa, & Ostensen, 2010), myasthenia gravis and lupus glomerulonephritis (Slater, Liang, McCune, Christman, & Laufer, 1999;Wetzels, 2004). CYPinduced gonadal toxicity in humans (Dooley & Nair, 2008;Wetzels, 2004) and rodents (Çeribaşi, Türk, Sönmez, Sakin, & Ateşşahin, 2010;El Tawab, Shahin, & AbdelMohsen, 2014;Kim et al, 2013) is associated with oligospermia, azoospermia, alterations in gonadotrophin, alterations in testosterone levels/oxidative stress parameters and testicular tissue toxicity. CYP disrupts cell growth, differentiation and function, mainly by cross-linking DNA strands (Vernet, Aitken, & Drevet, 2004); and there are suggestions that CYP (either alone, or in combination with other agents) damages the germinal epithelium (Ginsberg, 2012).…”

“…Its effect on rapidly proliferating tissues (which is predominant in the gonad) has also been considered as the basis of either its therapeutic or toxic potential (Ghobadi, Moloudizargari, Asghari, & Abdollahi, 2017). CYPinduced gonadal toxicity in humans (Dooley & Nair, 2008;Wetzels, 2004) and rodents (Çeribaşi, Türk, Sönmez, Sakin, & Ateşşahin, 2010;El Tawab, Shahin, & AbdelMohsen, 2014;Kim et al, 2013) is associated with oligospermia, azoospermia, alterations in gonadotrophin, alterations in testosterone levels/oxidative stress parameters and testicular tissue toxicity. In male rodents, there have been reports of reduction in testicular weight/testosterone levels and infertility (El-Alfy, Isa, Mahmoud, & Emam, 2013;Elangovan, Chiou, Tzeng, & Chu, 2006;Singh, Lata, & Tiwari, 2015) following administration of CYP.…”

Histological Comparative Study between the Possible Effect of Stem Cells and α- Lipoic Acid on Rat Testes Treated by Cyclophosphamide

“…The application of CP as anticancer and immunosuppressive agent is commonly accompanied by reproductive toxicity [22] . Human studies showed CP chemotherapy resulted in prolonged male gonadal injury, diminished testosterone synthesis and depletion of spermatogonia leading to infertility [23] .…”